The Fusion Activity of IM30 Rings Involves Controlled Unmasking of the Fusogenic Core
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Adrien Thurotte
- Dirk Schneider
- DOI
- 10.3389/fpls.2019.00108
- eISSN
- 1664-462X
- Zeitschrift
- Frontiers in Plant Science
- Online publication date
- 2019
- Status
- Published online
- Herausgeber
- Frontiers Media SA
- Herausgeber URL
- http://dx.doi.org/10.3389/fpls.2019.00108
- Datum der Datenerfassung
- 2019
- Titel
- The Fusion Activity of IM30 Rings Involves Controlled Unmasking of the Fusogenic Core
- Ausgabe der Zeitschrift
- 10
Data source: Crossref
- Other metadata sources:
-
- Abstract
- The <i>inner membrane-associated protein of 30 kDa</i> (IM30, also known as Vipp1) is required for thylakoid membrane biogenesis and maintenance in cyanobacteria and chloroplasts. The protein forms large rings of ∼2 MDa and triggers membrane fusion in presence of Mg<sup>2+</sup>. Based on the here presented observations, IM30 rings are built from dimers of dimers, and formation of these tetrameric building blocks is driven by interactions of the central coiled-coil, formed by helices 2 and 3, and stabilized via additional interactions mainly involving helix 1. Furthermore, helix 1 as well as C-terminal regions of IM30 together negatively regulate ring-ring contacts. We propose that IM30 rings represent the inactive form of IM30, and upon binding to negatively charged membrane surfaces, the here identified fusogenic core of IM30 rings eventually interacts with the lipid bilayer, resulting in membrane destabilization and membrane fusion. Unmasking of the IM30 fusogenic core likely is controlled by Mg<sup>2+</sup>, which triggers rearrangement of the IM30 ring structure.
- Addresses
- Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.
- Autoren
- Adrien Thurotte
- Dirk Schneider
- DOI
- 10.3389/fpls.2019.00108
- eISSN
- 1664-462X
- Externe Identifier
- PubMed Identifier: 30792728
- PubMed Central ID: PMC6374351
- Open access
- true
- ISSN
- 1664-462X
- Zeitschrift
- Frontiers in plant science
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2019
- Open access status
- Open Access
- Paginierung
- 108
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2019
- Titel
- The Fusion Activity of IM30 Rings Involves Controlled Unmasking of the Fusogenic Core.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 10
Files
https://www.frontiersin.org/articles/10.3389/fpls.2019.00108/pdf https://europepmc.org/articles/PMC6374351?pdf=render
Data source: Europe PubMed Central
- Abstract
- The inner membrane-associated protein of 30 kDa (IM30, also known as Vipp1) is required for thylakoid membrane biogenesis and maintenance in cyanobacteria and chloroplasts. The protein forms large rings of ∼2 MDa and triggers membrane fusion in presence of Mg2+. Based on the here presented observations, IM30 rings are built from dimers of dimers, and formation of these tetrameric building blocks is driven by interactions of the central coiled-coil, formed by helices 2 and 3, and stabilized via additional interactions mainly involving helix 1. Furthermore, helix 1 as well as C-terminal regions of IM30 together negatively regulate ring-ring contacts. We propose that IM30 rings represent the inactive form of IM30, and upon binding to negatively charged membrane surfaces, the here identified fusogenic core of IM30 rings eventually interacts with the lipid bilayer, resulting in membrane destabilization and membrane fusion. Unmasking of the IM30 fusogenic core likely is controlled by Mg2+, which triggers rearrangement of the IM30 ring structure.
- Date of acceptance
- 2019
- Autoren
- Adrien Thurotte
- Dirk Schneider
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/30792728
- DOI
- 10.3389/fpls.2019.00108
- Externe Identifier
- PubMed Central ID: PMC6374351
- ISSN
- 1664-462X
- Zeitschrift
- Front Plant Sci
- Schlüsselwörter
- IM30
- PspA
- Vipp1
- chloroplast
- cyanobacteria
- membrane biogenesis
- membrane fusion
- thylakoid membrane
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- 108
- Datum der Veröffentlichung
- 2019
- Status
- Published online
- Titel
- The Fusion Activity of IM30 Rings Involves Controlled Unmasking of the Fusogenic Core.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 10
Data source: PubMed
- Author's licence
- CC-BY
- Autoren
- Adrien Thurotte
- Dirk Schneider
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- JGU-Publikationen
- Resource version
- Published version
- URN
- urn:nbn:de:hebis:77-publ-589703
- DOI
- 10.3389/fpls.2019.00108
- Funding acknowledgements
- DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1664-462X
- Zeitschrift
- Frontiers in plant science
- Schlüsselwörter
- 500 Naturwissenschaften
- 500 Natural sciences and mathematics
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- Art. 108
- Datum der Veröffentlichung
- 2019
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/482
- Herausgeber
- Frontiers Media
- Herausgeber URL
- http://dx.doi.org/10.3389/fpls.2019.00108
- Datum der Datenerfassung
- 2019
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2019
- Zugang
- Public
- Titel
- The fusion activity of IM30 rings involves controlled unmasking of the fusogenic core
- Ausgabe der Zeitschrift
- 10
Files
58970.pdf
Data source: OPENSCIENCE.UB
- Beziehungen:
- Property of