Hormone-Inducible Transport Reporter Assay to Study Nuclear Import Defects in Neurodegenerative Diseases
- Publication type:
- Chapter
- Metadata:
-
- Autoren
- Saskia Hutten
- Dorothee Dormann
- DOI
- 10.1007/978-1-0716-2337-4_5
- ISBN-13
- 9781071623367
- Online publication date
- 2022
- Paginierung
- 81 - 90
- Buchtitel
- Methods in Molecular Biology
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Herausgeber
- Springer US
- Herausgeber URL
- http://dx.doi.org/10.1007/978-1-0716-2337-4_5
- Datum der Datenerfassung
- 2022
- Titel
- Hormone-Inducible Transport Reporter Assay to Study Nuclear Import Defects in Neurodegenerative Diseases
Data source: Crossref
- Other metadata sources:
-
- Abstract
- In the recent years, defective nuclear import has emerged as an important pathomechanism of neurodegenerative diseases, particularly in amyotrophic lateral sclerosis (ALS). Here, specific nuclear RNA binding proteins (RBPs) mislocalize and aggregate in the cytoplasm of neurons and glial cells in degenerating brain regions. Bona fide transport assays that measure nuclear import in a quantitative manner allow one to distinguish whether disease-linked RBP mutations that cause cytosolic RBP mislocalization directly result in reduced nuclear import or cause increased cytoplasmic localization of the RBP through other mechanisms. Here we describe the quantitative analysis of nuclear import rates of RBPs using a hormone-inducible system by live cell imaging.
- Addresses
- Johannes Gutenberg Universität Mainz, Institute of Molecular Physiology, Mainz, Germany. shutten@uni-mainz.de.
- Autoren
- Saskia Hutten
- Dorothee Dormann
- DOI
- 10.1007/978-1-0716-2337-4_5
- Open access
- false
- Schlüsselwörter
- Cytoplasm
- Humans
- Amyotrophic Lateral Sclerosis
- Neurodegenerative Diseases
- Hormones
- RNA-Binding Proteins
- Green Fluorescent Proteins
- Microscopy, Fluorescence
- Luminescent Measurements
- Active Transport, Cell Nucleus
- Medium
- Paginierung
- 81 - 90
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Datum der Datenerfassung
- 2022
- Titel
- Hormone-Inducible Transport Reporter Assay to Study Nuclear Import Defects in Neurodegenerative Diseases.
- Ausgabe der Zeitschrift
- 2502
Data source: Europe PubMed Central
- Abstract
- In the recent years, defective nuclear import has emerged as an important pathomechanism of neurodegenerative diseases, particularly in amyotrophic lateral sclerosis (ALS). Here, specific nuclear RNA binding proteins (RBPs) mislocalize and aggregate in the cytoplasm of neurons and glial cells in degenerating brain regions. Bona fide transport assays that measure nuclear import in a quantitative manner allow one to distinguish whether disease-linked RBP mutations that cause cytosolic RBP mislocalization directly result in reduced nuclear import or cause increased cytoplasmic localization of the RBP through other mechanisms. Here we describe the quantitative analysis of nuclear import rates of RBPs using a hormone-inducible system by live cell imaging.
- Autoren
- Saskia Hutten
- Dorothee Dormann
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/35412232
- DOI
- 10.1007/978-1-0716-2337-4_5
- Schlüsselwörter
- Hormone-inducible
- Live cell imaging
- Neurodegeneration
- Nuclear import
- Nuclear localization sequence
- Quantitative transport assay
- RNA binding protein
- Active Transport, Cell Nucleus
- Amyotrophic Lateral Sclerosis
- Cytoplasm
- Green Fluorescent Proteins
- Hormones
- Humans
- Luminescent Measurements
- Microscopy, Fluorescence
- Neurodegenerative Diseases
- RNA-Binding Proteins
- Paginierung
- 81 - 90
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Titel
- Hormone-Inducible Transport Reporter Assay to Study Nuclear Import Defects in Neurodegenerative Diseases.
- Ausgabe der Zeitschrift
- 2502
Data source: PubMed
- Beziehungen:
- Property of