Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells
- Publication type:
- Chapter
- Metadata:
-
- Autoren
- Lara A Gruijs da Silva
- Dorothee Dormann
- DOI
- 10.1007/978-1-0716-2663-4_16
- ISBN-13
- 9781071626627
- Online publication date
- 2022
- Paginierung
- 325 - 339
- Buchtitel
- Methods in Molecular Biology
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Herausgeber
- Springer US
- Herausgeber URL
- http://dx.doi.org/10.1007/978-1-0716-2663-4_16
- Datum der Datenerfassung
- 2022
- Titel
- Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells
Data source: Crossref
- Other metadata sources:
-
- Abstract
- In the last years, RNA-binding proteins (RBPs) have been highlighted for their capacity to undergo liquid-liquid phase separation (LLPS). Aberrant phase transitions of RBPs from a liquid to a solid state are believed to underlie the formation of pathological RBP aggregates in several neurodegenerative diseases. Both in the physiological and the disease state, RBPs are often decorated with diverse posttranslational modifications (PTMs) that can influence the phase separation behavior, the physiological function, and the pathological behavior of the RBP. Here we describe two simple methods, sedimentation assays in vitro and in cells, that allow the analysis of RBP solubility as a measure of RBP phase separation in the absence or presence of a certain PTM.
- Addresses
- Johannes Gutenberg-Universität (JGU), Biocenter, Institute of Molecular Physiology, Mainz, Germany.
- Autoren
- Lara A Gruijs da Silva
- Dorothee Dormann
- DOI
- 10.1007/978-1-0716-2663-4_16
- Open access
- false
- Schlüsselwörter
- Cytoplasmic Granules
- Humans
- Neurodegenerative Diseases
- RNA-Binding Proteins
- Protein Processing, Post-Translational
- Medium
- Paginierung
- 325 - 339
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Datum der Datenerfassung
- 2022
- Titel
- Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells.
- Ausgabe der Zeitschrift
- 2563
Data source: Europe PubMed Central
- Abstract
- In the last years, RNA-binding proteins (RBPs) have been highlighted for their capacity to undergo liquid-liquid phase separation (LLPS). Aberrant phase transitions of RBPs from a liquid to a solid state are believed to underlie the formation of pathological RBP aggregates in several neurodegenerative diseases. Both in the physiological and the disease state, RBPs are often decorated with diverse posttranslational modifications (PTMs) that can influence the phase separation behavior, the physiological function, and the pathological behavior of the RBP. Here we describe two simple methods, sedimentation assays in vitro and in cells, that allow the analysis of RBP solubility as a measure of RBP phase separation in the absence or presence of a certain PTM.
- Autoren
- Lara A Gruijs da Silva
- Dorothee Dormann
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/36227481
- DOI
- 10.1007/978-1-0716-2663-4_16
- Schlüsselwörter
- Liquid-liquid phase separation (LLPS)
- Neurodegeneration
- Phosphorylation
- Posttranslational modifications (PTMs)
- RNA-binding proteins (RBPs)
- TAR DNA-binding protein of 43 kDa (TDP-43)
- Cytoplasmic Granules
- Humans
- Neurodegenerative Diseases
- Protein Processing, Post-Translational
- RNA-Binding Proteins
- Paginierung
- 325 - 339
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Titel
- Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells.
- Ausgabe der Zeitschrift
- 2563
Data source: PubMed
- Beziehungen:
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