Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies

Publication type:
Chapter
Metadata:
Autoren
  • Martin Schaefer
  • Stefan Meldau
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000411979900011&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1007/978-1-4939-6831-2_10
ISBN-13
978-1-4939-6829-9
Schlüsselwörter
  • Cytokinins
  • Isopentenyl transferase
  • pOp6
  • LhGR
  • Dexamethasone
  • Stress response
  • Ecology
  • Fieldwork
Paginierung
127 - 139
Buchtitel
AUXINS AND CYTOKININS IN PLANT BIOLOGY: METHODS AND PROTOCOLS
Datum der Veröffentlichung
2017
Status
Published
Titel
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies
Ausgabe der Zeitschrift
1569

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
DOI
10.1007/978-1-4939-6831-2_10
ISBN-13
9781493968299
Online publication date
2017
Paginierung
127 - 139
Buchtitel
Methods in Molecular Biology
Datum der Veröffentlichung
2017
Status
Published
Herausgeber
Springer New York
Herausgeber URL
http://dx.doi.org/10.1007/978-1-4939-6831-2_10
Datum der Datenerfassung
2017
Titel
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies

Data source: Crossref

Abstract
Although many established tools for cytokinin (CK) pathway manipulations are well suitable for the analysis of molecular interactions, their use on a whole plant scale is often limited by the induction of severe developmental defects. To circumvent this problem, different methods were developed that allow for a more precise manipulation of the CK pathway. Here we present one of these systems, the pOp6/LhGR system for chemically inducible gene expression. This system allows regulation on a spatial, temporal, and quantitative scale and therefore provides a superior tool for analyzing the role of CKs in the interactions of plants with their environment. The pOp6/LhGR system was tested for RNAi-mediated gene silencing and heterologous gene expression and was successfully used for CK pathway manipulations in different model organisms (Arabidopsis thaliana, Nicotiana tabaccum, Nicotiana attenuata, Citrus sinensis × C. trifoliate). Here we describe specific aspects of the screening procedure and present an experimental setup that can not only be used in the laboratory but is also applicable under field conditions.
Addresses
  • Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Str.8, 07745, Jena, Germany.
Autoren
DOI
10.1007/978-1-4939-6831-2_10
Open access
false
Schlüsselwörter
  • Plants
  • Plants, Genetically Modified
  • Cytokinins
  • Genetic Engineering
  • Signal Transduction
  • Gene Expression Regulation, Plant
  • Phenotype
  • Genetic Testing
  • Herbivory
Medium
Print
Paginierung
127 - 139
Datum der Veröffentlichung
2017
Status
Published
Datum der Datenerfassung
2017
Titel
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies.
Ausgabe der Zeitschrift
1569

Data source: Europe PubMed Central

Abstract
Although many established tools for cytokinin (CK) pathway manipulations are well suitable for the analysis of molecular interactions, their use on a whole plant scale is often limited by the induction of severe developmental defects. To circumvent this problem, different methods were developed that allow for a more precise manipulation of the CK pathway. Here we present one of these systems, the pOp6/LhGR system for chemically inducible gene expression. This system allows regulation on a spatial, temporal, and quantitative scale and therefore provides a superior tool for analyzing the role of CKs in the interactions of plants with their environment. The pOp6/LhGR system was tested for RNAi-mediated gene silencing and heterologous gene expression and was successfully used for CK pathway manipulations in different model organisms (Arabidopsis thaliana, Nicotiana tabaccum, Nicotiana attenuata, Citrus sinensis × C. trifoliate). Here we describe specific aspects of the screening procedure and present an experimental setup that can not only be used in the laboratory but is also applicable under field conditions.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/28265993
DOI
10.1007/978-1-4939-6831-2_10
Schlüsselwörter
  • Cytokinins
  • Dexamethasone
  • Ecology
  • Fieldwork
  • Isopentenyl transferase
  • LhGR
  • Stress response
  • pOp6
  • Cytokinins
  • Gene Expression Regulation, Plant
  • Genetic Engineering
  • Genetic Testing
  • Herbivory
  • Phenotype
  • Plants
  • Plants, Genetically Modified
  • Signal Transduction
Paginierung
127 - 139
Datum der Veröffentlichung
2017
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2018
Titel
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies.
Ausgabe der Zeitschrift
1569

Data source: PubMed

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