Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association

Publication type:
Journal article
Metadata:
Autoren
  • Elisabeth Mindt
  • Ming Wang
  • Martin Schaefer
  • Rayko Halitschke
  • Ian T Baldwin
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000476648400013&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.21769/BioProtoc.3301
eISSN
2331-8325
Externe Identifier
  • Clarivate Analytics Document Solution ID: IK5TT
  • PubMed Identifier: 33654814
Ausgabe der Veröffentlichung
14
Zeitschrift
BIO-PROTOCOL
Schlüsselwörter
  • Arbuscular mycorrhizal fungi
  • AMF
  • Fungus-plant symbiosis
  • Leaf biomarker
  • Shoot biomarker
  • Blumenol derivatives
  • UHPLC-MS/MS technique
Artikelnummer
ARTN e3301
Datum der Veröffentlichung
2019
Status
Published
Titel
Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association
Sub types
  • Article
Ausgabe der Zeitschrift
9

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
DOI
10.21769/bioprotoc.3301
ISSN
2331-8325
Ausgabe der Veröffentlichung
14
Zeitschrift
BIO-PROTOCOL
Sprache
en
Datum der Veröffentlichung
2019
Status
Published
Herausgeber
Bio-Protocol, LLC
Herausgeber URL
http://dx.doi.org/10.21769/bioprotoc.3301
Datum der Datenerfassung
2024
Titel
Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association
Ausgabe der Zeitschrift
9

Data source: Crossref

Abstract
Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, efficient characterization methods are required to monitor and quantify plant-AMF colonization. Existing methods, based on destructive sampling and elaborate root tissue analysis, are of limited value for high-throughput (HTP) screening. Here we describe a detailed protocol for the HTP quantification of blumenol derivatives in leaves by a simple extraction procedure and sensitive liquid chromatography mass spectrometry (LC/MS) analysis as accurate proxies of root AMF-associations in both model plants and economically relevant crops.
Addresses
  • Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany.
Autoren
DOI
10.21769/bioprotoc.3301
eISSN
2331-8325
Externe Identifier
  • PubMed Identifier: 33654814
  • PubMed Central ID: PMC7854195
Funding acknowledgements
  • European Research Council: 293926
Open access
true
ISSN
2331-8325
Ausgabe der Veröffentlichung
14
Zeitschrift
Bio-protocol
Sprache
eng
Medium
Electronic-eCollection
Online publication date
2019
Open access status
Open Access
Paginierung
e3301
Datum der Veröffentlichung
2019
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2021
Titel
Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association.
Sub types
  • methods-article
  • Journal Article
Ausgabe der Zeitschrift
9

Files

https://bio-protocol.org/pdf/Bio-protocol3301.pdf https://europepmc.org/articles/PMC7854195?pdf=render

Data source: Europe PubMed Central

Abstract
Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, efficient characterization methods are required to monitor and quantify plant-AMF colonization. Existing methods, based on destructive sampling and elaborate root tissue analysis, are of limited value for high-throughput (HTP) screening. Here we describe a detailed protocol for the HTP quantification of blumenol derivatives in leaves by a simple extraction procedure and sensitive liquid chromatography mass spectrometry (LC/MS) analysis as accurate proxies of root AMF-associations in both model plants and economically relevant crops.
Date of acceptance
2019
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/33654814
DOI
10.21769/BioProtoc.3301
eISSN
2331-8325
Externe Identifier
  • PubMed Central ID: PMC7854195
Ausgabe der Veröffentlichung
14
Zeitschrift
Bio Protoc
Schlüsselwörter
  • AMF
  • Arbuscular mycorrhizal fungi
  • Blumenol derivatives
  • Fungus-plant symbiosis
  • Leaf biomarker
  • Shoot biomarker
  • UHPLC-MS/MS technique
Sprache
eng
Country
United States
Paginierung
e3301
PII
e3301
Datum der Veröffentlichung
2019
Status
Published online
Titel
Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
9

Data source: PubMed

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