High-throughput quantification of more than 100 primary- and secondary-metabolites, and phytohormones by a single solid-phase extraction based sample preparation with analysis by UHPLC-HESI-MS/MS

Publication type:
Journal article
Metadata:
Autoren
  • Martin Schaefer
  • Christoph Bruetting
  • Ian T Baldwin
  • Mario Kallenbach
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000376675100001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1186/s13007-016-0130-x
Externe Identifier
  • Clarivate Analytics Document Solution ID: DM9HD
  • PubMed Identifier: 27239220
ISSN
1746-4811
Zeitschrift
PLANT METHODS
Schlüsselwörter
  • Phytohormones
  • Jasmonate
  • Salicylic acid
  • Abscisic acid
  • Gibberellin
  • Auxin
  • Cytokinin
  • Secondary metabolites
  • Primary metabolites
  • Solid-phase extraction
Artikelnummer
ARTN 30
Datum der Veröffentlichung
2016
Status
Published
Titel
High-throughput quantification of more than 100 primary- and secondary-metabolites, and phytohormones by a single solid-phase extraction based sample preparation with analysis by UHPLC-HESI-MS/MS
Sub types
  • Article
Ausgabe der Zeitschrift
12

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
DOI
10.1186/s13007-016-0130-x
eISSN
1746-4811
Ausgabe der Veröffentlichung
1
Zeitschrift
Plant Methods
Sprache
en
Artikelnummer
30
Online publication date
2016
Datum der Veröffentlichung
2016
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1186/s13007-016-0130-x
Datum der Datenerfassung
2022
Titel
High-throughput quantification of more than 100 primary- and secondary-metabolites, and phytohormones by a single solid-phase extraction based sample preparation with analysis by UHPLC–HESI–MS/MS
Ausgabe der Zeitschrift
12

Data source: Crossref

Abstract
<h4>Background</h4>Plant metabolites are commonly functionally classified, as defense- or growth-related phytohormones, primary and specialized metabolites, and so forth. Analytical procedures for the quantifications of these metabolites are challenging because the metabolites can vary over several orders of magnitude in concentrations in the same tissues and have very different chemical characteristics. Plants clearly adjust their metabolism to respond to their prevailing circumstances in very sophisticated ways that blur the boundaries among these functional or chemically defined classifications. But if plant biologists want to better understand the processes that are important for a plant's adaptation to its environment, procedures are needed that can provide simultaneous quantifications of the large range of metabolites that have the potential to play central roles in these adjustments in a cost and time effective way and with a low sample consumption.<h4>Results</h4>Here we present a method that combines well-established methods for the targeted analysis of phytohormones, including jasmonates, salicylic acid, abscisic acid, gibberellins, auxins and cytokinins, and extends it to the analysis of inducible and constitutive defense compounds, as well as the primary metabolites involved in the biosynthesis of specialized metabolites and responsible for nutritional quality (e.g., sugars and amino acids). The method is based on a single extraction of 10-100 mg of tissue and allows a broad quantitative screening of metabolites optimized by their chemical characteristics and concentrations, thereby providing a high throughput analysis unbiased by the putative functional attributes of the metabolites. The tissues of Nicotiana attenuata which accumulate high levels of nicotine and diterpene glycosides, provide a challenging matrix that thwarts quantitative analysis; the analysis of various tissues of this plant are used to illustrate the robustness of the procedure.<h4>Conclusions</h4>The method described has the potential to unravel various, until now overlooked interactions among different sectors of plant metabolism in a high throughput manner. Additionally, the method could be particularly beneficial as screening method in forward genetic approaches, as well as for the investigation of plants from natural populations that likely differ in metabolic traits.
Addresses
  • Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Str. 8, 07745 Jena, Germany.
Autoren
DOI
10.1186/s13007-016-0130-x
eISSN
1746-4811
Externe Identifier
  • PubMed Identifier: 27239220
  • PubMed Central ID: PMC4882772
Funding acknowledgements
  • European Research Council: 293926
  • Collaborative Research Centres: SFB 1127
  • Max-Planck-Gesellschaft:
Open access
true
ISSN
1746-4811
Zeitschrift
Plant methods
Sprache
eng
Medium
Electronic-eCollection
Online publication date
2016
Open access status
Open Access
Paginierung
30
Datum der Veröffentlichung
2016
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2016
Titel
High-throughput quantification of more than 100 primary- and secondary-metabolites, and phytohormones by a single solid-phase extraction based sample preparation with analysis by UHPLC-HESI-MS/MS.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
12

Files

https://plantmethods.biomedcentral.com/track/pdf/10.1186/s13007-016-0130-x https://europepmc.org/articles/PMC4882772?pdf=render

Data source: Europe PubMed Central

Abstract
BACKGROUND: Plant metabolites are commonly functionally classified, as defense- or growth-related phytohormones, primary and specialized metabolites, and so forth. Analytical procedures for the quantifications of these metabolites are challenging because the metabolites can vary over several orders of magnitude in concentrations in the same tissues and have very different chemical characteristics. Plants clearly adjust their metabolism to respond to their prevailing circumstances in very sophisticated ways that blur the boundaries among these functional or chemically defined classifications. But if plant biologists want to better understand the processes that are important for a plant's adaptation to its environment, procedures are needed that can provide simultaneous quantifications of the large range of metabolites that have the potential to play central roles in these adjustments in a cost and time effective way and with a low sample consumption. RESULTS: Here we present a method that combines well-established methods for the targeted analysis of phytohormones, including jasmonates, salicylic acid, abscisic acid, gibberellins, auxins and cytokinins, and extends it to the analysis of inducible and constitutive defense compounds, as well as the primary metabolites involved in the biosynthesis of specialized metabolites and responsible for nutritional quality (e.g., sugars and amino acids). The method is based on a single extraction of 10-100 mg of tissue and allows a broad quantitative screening of metabolites optimized by their chemical characteristics and concentrations, thereby providing a high throughput analysis unbiased by the putative functional attributes of the metabolites. The tissues of Nicotiana attenuata which accumulate high levels of nicotine and diterpene glycosides, provide a challenging matrix that thwarts quantitative analysis; the analysis of various tissues of this plant are used to illustrate the robustness of the procedure. CONCLUSIONS: The method described has the potential to unravel various, until now overlooked interactions among different sectors of plant metabolism in a high throughput manner. Additionally, the method could be particularly beneficial as screening method in forward genetic approaches, as well as for the investigation of plants from natural populations that likely differ in metabolic traits.
Date of acceptance
2016
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/27239220
DOI
10.1186/s13007-016-0130-x
Externe Identifier
  • PubMed Central ID: PMC4882772
Funding acknowledgements
  • European Research Council: 293926
ISSN
1746-4811
Zeitschrift
Plant Methods
Schlüsselwörter
  • Abscisic acid
  • Auxin
  • Cytokinin
  • Gibberellin
  • Jasmonate
  • Phytohormones
  • Primary metabolites
  • Salicylic acid
  • Secondary metabolites
  • Solid-phase extraction
Sprache
eng
Country
England
Paginierung
30
PII
130
Datum der Veröffentlichung
2016
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
High-throughput quantification of more than 100 primary- and secondary-metabolites, and phytohormones by a single solid-phase extraction based sample preparation with analysis by UHPLC-HESI-MS/MS.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
12

Data source: PubMed

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