Novel bioreactor internals for the cultivation of spore-forming fungi in pellet form
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Winda Soerjawinata
- Isabelle Kockler
- Lars Wommer
- Robert Frank
- Anja Schueffler
- Tanja Schirmeister
- Roland Ulber
- Percy Kampeis
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000797232200001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1002/elsc.202100094
- eISSN
- 1618-2863
- Externe Identifier
- Clarivate Analytics Document Solution ID: 2Y9IS
- PubMed Identifier: 35865648
- ISSN
- 1618-0240
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- ENGINEERING IN LIFE SCIENCES
- Schlüsselwörter
- bioreactor internals
- fungal growth
- fungal pellets
- repeated-batch fermentation
- sporulation conditions
- Paginierung
- 474 - 483
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Titel
- Novel bioreactor internals for the cultivation of spore-forming fungi in pellet form
- Sub types
- Article
- Ausgabe der Zeitschrift
- 22
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>This study introduced an automated long‐term fermentation process for fungals grown in pellet form. The goal was to reduce the overgrowth of bioreactor internals and sensors while better rheological properties in the fermentation broth, such as oxygen transfer and mixing time, can be achieved. Because this could not be accomplished with continuous culture and fed‐batch fermentation, repeated‐batch fermentation was implemented with the help of additional bioreactor internals (“sporulation supports”). This should capture some biomass during fermentation. After harvesting the suspended biomass, intermediate cleaning was performed using a cleaning device. The biomass retained on the sporulation support went through the sporulation phase. The spores were subsequently used as inocula for the next batch. The reason for this approach was that the retained pellets could otherwise cause problems (<jats:italic>e.g</jats:italic>., overgrowth on sensors) in subsequent batches because the fungus would then show undesirable hyphal growth. Various sporulation supports were tested for sufficient biomass fixation to start the next batch. A reproducible spore concentration within the range of the requirements could be achieved by adjusting the sporulation support (design and construction material), and an intermediate cleaning adapted to this.</jats:p>
- Autoren
- Winda Soerjawinata
- Isabelle Kockler
- Lars Wommer
- Robert Frank
- Anja Schüffler
- Tanja Schirmeister
- Roland Ulber
- Percy Kampeis
- DOI
- 10.1002/elsc.202100094
- eISSN
- 1618-2863
- ISSN
- 1618-0240
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- Engineering in Life Sciences
- Sprache
- en
- Online publication date
- 2022
- Paginierung
- 474 - 483
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/elsc.202100094
- Datum der Datenerfassung
- 2023
- Titel
- Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form
- Ausgabe der Zeitschrift
- 22
Data source: Crossref
- Abstract
- This study introduced an automated long-term fermentation process for fungals grown in pellet form. The goal was to reduce the overgrowth of bioreactor internals and sensors while better rheological properties in the fermentation broth, such as oxygen transfer and mixing time, can be achieved. Because this could not be accomplished with continuous culture and fed-batch fermentation, repeated-batch fermentation was implemented with the help of additional bioreactor internals ("sporulation supports"). This should capture some biomass during fermentation. After harvesting the suspended biomass, intermediate cleaning was performed using a cleaning device. The biomass retained on the sporulation support went through the sporulation phase. The spores were subsequently used as inocula for the next batch. The reason for this approach was that the retained pellets could otherwise cause problems (<i>e.g</i>., overgrowth on sensors) in subsequent batches because the fungus would then show undesirable hyphal growth. Various sporulation supports were tested for sufficient biomass fixation to start the next batch. A reproducible spore concentration within the range of the requirements could be achieved by adjusting the sporulation support (design and construction material), and an intermediate cleaning adapted to this.
- Addresses
- Institute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten-Weiersbach Germany.
- Autoren
- Winda Soerjawinata
- Isabelle Kockler
- Lars Wommer
- Robert Frank
- Anja Schüffler
- Tanja Schirmeister
- Roland Ulber
- Percy Kampeis
- DOI
- 10.1002/elsc.202100094
- eISSN
- 1618-2863
- Externe Identifier
- PubMed Identifier: 35865648
- PubMed Central ID: PMC9288991
- Open access
- true
- ISSN
- 1618-0240
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- Engineering in life sciences
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2022
- Open access status
- Open Access
- Paginierung
- 474 - 483
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2022
- Titel
- Novel bioreactor internals for the cultivation of spore-forming fungi in pellet form.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 22
Files
https://europepmc.org/articles/PMC9288991?pdf=render
Data source: Europe PubMed Central
- Abstract
- This study introduced an automated long-term fermentation process for fungals grown in pellet form. The goal was to reduce the overgrowth of bioreactor internals and sensors while better rheological properties in the fermentation broth, such as oxygen transfer and mixing time, can be achieved. Because this could not be accomplished with continuous culture and fed-batch fermentation, repeated-batch fermentation was implemented with the help of additional bioreactor internals ("sporulation supports"). This should capture some biomass during fermentation. After harvesting the suspended biomass, intermediate cleaning was performed using a cleaning device. The biomass retained on the sporulation support went through the sporulation phase. The spores were subsequently used as inocula for the next batch. The reason for this approach was that the retained pellets could otherwise cause problems (e.g., overgrowth on sensors) in subsequent batches because the fungus would then show undesirable hyphal growth. Various sporulation supports were tested for sufficient biomass fixation to start the next batch. A reproducible spore concentration within the range of the requirements could be achieved by adjusting the sporulation support (design and construction material), and an intermediate cleaning adapted to this.
- Date of acceptance
- 2022
- Autoren
- Winda Soerjawinata
- Isabelle Kockler
- Lars Wommer
- Robert Frank
- Anja Schüffler
- Tanja Schirmeister
- Roland Ulber
- Percy Kampeis
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/35865648
- DOI
- 10.1002/elsc.202100094
- Externe Identifier
- PubMed Central ID: PMC9288991
- ISSN
- 1618-0240
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- Eng Life Sci
- Schlüsselwörter
- bioreactor internals
- fungal growth
- fungal pellets
- repeated‐batch fermentation
- sporulation conditions
- Sprache
- eng
- Country
- Germany
- Paginierung
- 474 - 483
- PII
- ELSC1488
- Datum der Veröffentlichung
- 2022
- Status
- Published online
- Titel
- Novel bioreactor internals for the cultivation of spore-forming fungi in pellet form.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 22
Data source: PubMed
- Beziehungen:
- Property of