Cytotoxicity, acute and sub-chronic toxicities of the leaves of Bauhinia thonningii (Schumach.) Milne-Redh. (Caesalpiniaceae)
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Valaire Y Matieta
- Armelle T Mbaveng
- Guy R Sado Nouemsi
- Simplice B Tankeo
- Gabriel T Kamsu
- Paul Nayim
- Alain M Lannang
- Ilhami Celik
- Thomas Efferth
- Victor Kuete
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:001072575700001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1186/s12906-023-04172-9
- eISSN
- 2662-7671
- Externe Identifier
- Clarivate Analytics Document Solution ID: S6XL1
- PubMed Identifier: 37752510
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC COMPLEMENTARY MEDICINE AND THERAPIES
- Schlüsselwörter
- 6,8-C-dimethylkaempferol-3-methyl ether
- Cancer
- Cytotoxicity
- Phytochemistry
- Bauhinia thonningii
- Toxicity
- Artikelnummer
- ARTN 341
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Titel
- Cytotoxicity, acute and sub-chronic toxicities of the leaves of <i>Bauhinia thonningii</i> (Schumach.) Milne-Redh. (Caesalpiniaceae)
- Sub types
- Article
- Ausgabe der Zeitschrift
- 23
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p><jats:italic>Bauhinia thonningii</jats:italic> is a plant traditionally used against many human diseases such as gastric ulcers, fever, inflammations, coughs, dysentery, diarrhea, and malaria. In the present investigation, the cytotoxicity of methanol extract of <jats:italic>Bauhinia thonningii</jats:italic> leaves (BTL), fractions and the isolated phytoconstituents was determined in a panel of 9 human cancer cell lines including drug sensitive and multidrug-resistant (MDR) phenotypes. The acute and sub-chronic oral toxicity of BTL was investigated as well.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>Compounds were isolated using chromatographic techniques while their chemical structures were determined using spectroscopic methods. The resazurin reduction assay (RRA) was used to evaluate the cytotoxicity of samples, propidium iodide (PI) for apoptosis, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining for mitochondrial membrane potential (MMP) analysis, 2´,7´-dichlorodihydrofluoresceine diacetate (H2DCFH-DA) staining for the quantification of reactive oxygen species (ROS), whereas Caspase Glo assays were combined by means of flow cytometry. Furthermore, the toxicological investigations were performed as recommended by the Organization for Economic Cooperation and Development (OECD).</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>The botanicals as well as 6-C-methylquercetin-3,7-dimethyl ether (2), quercetin-3-<jats:italic>O</jats:italic>-<jats:sub>L</jats:sub>-rhamnopyranoside (5), quercetin-3-<jats:italic>O-β</jats:italic>-glucopyranoside (6), 6,8-C-dimethylkaempferol 3,7-dimethyl ether (7), and 6,8-C-dimethylkaempferol-3-methyl ether (8) had promising cytotoxic effects in the 9 tested cancer cell lines. The IC<jats:sub>50</jats:sub> values below 20 µg/mL (botanicals) or 10 µM (compounds) on at least 1/9 tested cancer cell lines were considered. The best cytotoxic effects with IC<jats:sub>50</jats:sub> values below 5 µM were achieved with compounds 7 against CEM/ADR5000 leukemia cells (2.86 µM) and MDA-MB-231-<jats:italic>pcDNA</jats:italic> breast adenocarcinoma cells (1.93 µM) as well as 8 against CCRF-CEM leukemia cells (3.03 µM), CEM/ADR5000 cells (2.42 µM), MDA-MB-231-<jats:italic>pcDNA</jats:italic> (2.34 µM), and HCT116 <jats:italic>p53</jats:italic><jats:sup><jats:italic>−/−</jats:italic></jats:sup> cells (3.41 µM). BTL and compound 8 induced apoptotic cell death in CCRF-CEM cells through caspase activation, alteration of MMP, and increased ROS production. BTL did not cause any adverse effects in rats after a single administration at 5000 mg/kg or a repeated dose of 250 mg/kg body weight (b. w.).</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p><jats:italic>Bauhinia thonningii</jats:italic> and its constituents are sources of cytotoxic drugs that deserve more in-depth studies to develop novel antiproliferative phytomedicine to fight cancer including resistant phenotypes.</jats:p> </jats:sec>
- Autoren
- Valaire Y Matieta
- Armelle T Mbaveng
- Guy R Sado Nouemsi
- Simplice B Tankeo
- Gabriel T Kamsu
- Paul Nayim
- Alain M Lannang
- İlhami Çelik
- Thomas Efferth
- Victor Kuete
- DOI
- 10.1186/s12906-023-04172-9
- eISSN
- 2662-7671
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC Complementary Medicine and Therapies
- Sprache
- en
- Artikelnummer
- 341
- Online publication date
- 2023
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1186/s12906-023-04172-9
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity, acute and sub-chronic toxicities of the leaves of Bauhinia thonningii (Schumach.) Milne-Redh. (Caesalpiniaceae)
- Ausgabe der Zeitschrift
- 23
Data source: Crossref
- Abstract
- <h4>Background</h4>Bauhinia thonningii is a plant traditionally used against many human diseases such as gastric ulcers, fever, inflammations, coughs, dysentery, diarrhea, and malaria. In the present investigation, the cytotoxicity of methanol extract of Bauhinia thonningii leaves (BTL), fractions and the isolated phytoconstituents was determined in a panel of 9 human cancer cell lines including drug sensitive and multidrug-resistant (MDR) phenotypes. The acute and sub-chronic oral toxicity of BTL was investigated as well.<h4>Methods</h4>Compounds were isolated using chromatographic techniques while their chemical structures were determined using spectroscopic methods. The resazurin reduction assay (RRA) was used to evaluate the cytotoxicity of samples, propidium iodide (PI) for apoptosis, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining for mitochondrial membrane potential (MMP) analysis, 2´,7´-dichlorodihydrofluoresceine diacetate (H2DCFH-DA) staining for the quantification of reactive oxygen species (ROS), whereas Caspase Glo assays were combined by means of flow cytometry. Furthermore, the toxicological investigations were performed as recommended by the Organization for Economic Cooperation and Development (OECD).<h4>Results</h4>The botanicals as well as 6-C-methylquercetin-3,7-dimethyl ether (2), quercetin-3-O-<sub>L</sub>-rhamnopyranoside (5), quercetin-3-O-β-glucopyranoside (6), 6,8-C-dimethylkaempferol 3,7-dimethyl ether (7), and 6,8-C-dimethylkaempferol-3-methyl ether (8) had promising cytotoxic effects in the 9 tested cancer cell lines. The IC<sub>50</sub> values below 20 µg/mL (botanicals) or 10 µM (compounds) on at least 1/9 tested cancer cell lines were considered. The best cytotoxic effects with IC<sub>50</sub> values below 5 µM were achieved with compounds 7 against CEM/ADR5000 leukemia cells (2.86 µM) and MDA-MB-231-pcDNA breast adenocarcinoma cells (1.93 µM) as well as 8 against CCRF-CEM leukemia cells (3.03 µM), CEM/ADR5000 cells (2.42 µM), MDA-MB-231-pcDNA (2.34 µM), and HCT116 p53<sup>-/-</sup> cells (3.41 µM). BTL and compound 8 induced apoptotic cell death in CCRF-CEM cells through caspase activation, alteration of MMP, and increased ROS production. BTL did not cause any adverse effects in rats after a single administration at 5000 mg/kg or a repeated dose of 250 mg/kg body weight (b. w.).<h4>Conclusion</h4>Bauhinia thonningii and its constituents are sources of cytotoxic drugs that deserve more in-depth studies to develop novel antiproliferative phytomedicine to fight cancer including resistant phenotypes.
- Addresses
- Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
- Autoren
- Valaire Y Matieta
- Armelle T Mbaveng
- Guy R Sado Nouemsi
- Simplice B Tankeo
- Gabriel T Kamsu
- Paul Nayim
- Alain M Lannang
- İlhami Çelik
- Thomas Efferth
- Victor Kuete
- DOI
- 10.1186/s12906-023-04172-9
- eISSN
- 2662-7671
- Externe Identifier
- PubMed Identifier: 37752510
- PubMed Central ID: PMC10523748
- Open access
- true
- ISSN
- 2662-7671
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC complementary medicine and therapies
- Schlüsselwörter
- Animals
- Humans
- Rats
- Fabaceae
- Bauhinia
- Leukemia
- Reactive Oxygen Species
- Quercetin
- Caspases
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2023
- Open access status
- Open Access
- Paginierung
- 341
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity, acute and sub-chronic toxicities of the leaves of Bauhinia thonningii (Schumach.) Milne-Redh. (Caesalpiniaceae).
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 23
Files
https://bmccomplementmedtherapies.biomedcentral.com/counter/pdf/10.1186/s12906-023-04172-9 https://europepmc.org/articles/PMC10523748?pdf=render
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Bauhinia thonningii is a plant traditionally used against many human diseases such as gastric ulcers, fever, inflammations, coughs, dysentery, diarrhea, and malaria. In the present investigation, the cytotoxicity of methanol extract of Bauhinia thonningii leaves (BTL), fractions and the isolated phytoconstituents was determined in a panel of 9 human cancer cell lines including drug sensitive and multidrug-resistant (MDR) phenotypes. The acute and sub-chronic oral toxicity of BTL was investigated as well. METHODS: Compounds were isolated using chromatographic techniques while their chemical structures were determined using spectroscopic methods. The resazurin reduction assay (RRA) was used to evaluate the cytotoxicity of samples, propidium iodide (PI) for apoptosis, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining for mitochondrial membrane potential (MMP) analysis, 2´,7´-dichlorodihydrofluoresceine diacetate (H2DCFH-DA) staining for the quantification of reactive oxygen species (ROS), whereas Caspase Glo assays were combined by means of flow cytometry. Furthermore, the toxicological investigations were performed as recommended by the Organization for Economic Cooperation and Development (OECD). RESULTS: The botanicals as well as 6-C-methylquercetin-3,7-dimethyl ether (2), quercetin-3-O-L-rhamnopyranoside (5), quercetin-3-O-β-glucopyranoside (6), 6,8-C-dimethylkaempferol 3,7-dimethyl ether (7), and 6,8-C-dimethylkaempferol-3-methyl ether (8) had promising cytotoxic effects in the 9 tested cancer cell lines. The IC50 values below 20 µg/mL (botanicals) or 10 µM (compounds) on at least 1/9 tested cancer cell lines were considered. The best cytotoxic effects with IC50 values below 5 µM were achieved with compounds 7 against CEM/ADR5000 leukemia cells (2.86 µM) and MDA-MB-231-pcDNA breast adenocarcinoma cells (1.93 µM) as well as 8 against CCRF-CEM leukemia cells (3.03 µM), CEM/ADR5000 cells (2.42 µM), MDA-MB-231-pcDNA (2.34 µM), and HCT116 p53-/- cells (3.41 µM). BTL and compound 8 induced apoptotic cell death in CCRF-CEM cells through caspase activation, alteration of MMP, and increased ROS production. BTL did not cause any adverse effects in rats after a single administration at 5000 mg/kg or a repeated dose of 250 mg/kg body weight (b. w.). CONCLUSION: Bauhinia thonningii and its constituents are sources of cytotoxic drugs that deserve more in-depth studies to develop novel antiproliferative phytomedicine to fight cancer including resistant phenotypes.
- Date of acceptance
- 2023
- Autoren
- Valaire Y Matieta
- Armelle T Mbaveng
- Guy R Sado Nouemsi
- Simplice B Tankeo
- Gabriel T Kamsu
- Paul Nayim
- Alain M Lannang
- İlhami Çelik
- Thomas Efferth
- Victor Kuete
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/37752510
- DOI
- 10.1186/s12906-023-04172-9
- eISSN
- 2662-7671
- Externe Identifier
- PubMed Central ID: PMC10523748
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC Complement Med Ther
- Schlüsselwörter
- 6,8-C-dimethylkaempferol-3-methyl ether
- Bauhinia thonningii
- Cancer
- Cytotoxicity
- Phytochemistry
- Toxicity
- Humans
- Animals
- Rats
- Bauhinia
- Quercetin
- Reactive Oxygen Species
- Caspases
- Fabaceae
- Leukemia
- Sprache
- eng
- Country
- England
- Paginierung
- 341
- PII
- 10.1186/s12906-023-04172-9
- Datum der Veröffentlichung
- 2023
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2023
- Titel
- Cytotoxicity, acute and sub-chronic toxicities of the leaves of Bauhinia thonningii (Schumach.) Milne-Redh. (Caesalpiniaceae).
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 23
Data source: PubMed
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