A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Meret Huber
- Thomas Roder
- Sandra Irmisch
- Alexander Riedel
- Saskia Gablenz
- Julia Fricke
- Peter Rahfeld
- Michael Reichelt
- Christian Paetz
- Nicole Liechti
- Lingfei Hu
- Zoe Bont
- Ye Meng
- Wei Huang
- Christelle AM Robert
- Jonathan Gershenzon
- Matthias Erb
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000706543000001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.7554/eLife.68642
- Externe Identifier
- Clarivate Analytics Document Solution ID: WF8HT
- PubMed Identifier: 34632981
- ISSN
- 2050-084X
- Zeitschrift
- ELIFE
- Schlüsselwörter
- Melolontha melolontha
- Taraxacum officinale
- plant defense
- root herbivore
- sesquiterpene lactone
- beta-glucosidase
- Other
- Artikelnummer
- ARTN e68642
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite
- Sub types
- Article
- Ausgabe der Zeitschrift
- 10
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p>Gut enzymes can metabolize plant defense compounds and thereby affect the growth and fitness of insect herbivores. Whether these enzymes also influence feeding preference is largely unknown. We studied the metabolization of taraxinic acid β-D-glucopyranosyl ester (TA-G), a sesquiterpene lactone of the common dandelion (<jats:italic>Taraxacum officinale</jats:italic>) that deters its major root herbivore, the common cockchafer larva (<jats:italic>Melolontha melolontha</jats:italic>). We have demonstrated that TA-G is rapidly deglucosylated and conjugated to glutathione in the insect gut. A broad-spectrum <jats:italic>M. melolontha</jats:italic> β-glucosidase, Mm_bGlc17, is sufficient and necessary for TA-G deglucosylation. Using cross-species RNA interference, we have shown that Mm_bGlc17 reduces TA-G toxicity. Furthermore, Mm_bGlc17 is required for the preference of <jats:italic>M. melolontha</jats:italic> larvae for TA-G-deficient plants. Thus, herbivore metabolism modulates both the toxicity and deterrence of a plant defense compound. Our work illustrates the multifaceted roles of insect digestive enzymes as mediators of plant-herbivore interactions.</jats:p>
- Date of acceptance
- 2021
- Autoren
- Meret Huber
- Thomas Roder
- Sandra Irmisch
- Alexander Riedel
- Saskia Gablenz
- Julia Fricke
- Peter Rahfeld
- Michael Reichelt
- Christian Paetz
- Nicole Liechti
- Lingfei Hu
- Zoe Bont
- Ye Meng
- Wei Huang
- Christelle AM Robert
- Jonathan Gershenzon
- Matthias Erb
- DOI
- 10.7554/elife.68642
- eISSN
- 2050-084X
- Zeitschrift
- eLife
- Sprache
- en
- Online publication date
- 2021
- Status
- Published online
- Herausgeber
- eLife Sciences Publications, Ltd
- Herausgeber URL
- http://dx.doi.org/10.7554/elife.68642
- Datum der Datenerfassung
- 2023
- Titel
- A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite
- Ausgabe der Zeitschrift
- 10
Data source: Crossref
- Abstract
- Gut enzymes can metabolize plant defense compounds and thereby affect the growth and fitness of insect herbivores. Whether these enzymes also influence feeding preference is largely unknown. We studied the metabolization of taraxinic acid β-D-glucopyranosyl ester (TA-G), a sesquiterpene lactone of the common dandelion (<i>Taraxacum officinale</i>) that deters its major root herbivore, the common cockchafer larva (<i>Melolontha melolontha</i>). We have demonstrated that TA-G is rapidly deglucosylated and conjugated to glutathione in the insect gut. A broad-spectrum <i>M. melolontha</i> β-glucosidase, Mm_bGlc17, is sufficient and necessary for TA-G deglucosylation. Using cross-species RNA interference, we have shown that Mm_bGlc17 reduces TA-G toxicity. Furthermore, Mm_bGlc17 is required for the preference of <i>M. melolontha</i> larvae for TA-G-deficient plants. Thus, herbivore metabolism modulates both the toxicity and deterrence of a plant defense compound. Our work illustrates the multifaceted roles of insect digestive enzymes as mediators of plant-herbivore interactions.
- Addresses
- Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany.
- Autoren
- Meret Huber
- Thomas Roder
- Sandra Irmisch
- Alexander Riedel
- Saskia Gablenz
- Julia Fricke
- Peter Rahfeld
- Michael Reichelt
- Christian Paetz
- Nicole Liechti
- Lingfei Hu
- Zoe Bont
- Ye Meng
- Wei Huang
- Christelle Am Robert
- Jonathan Gershenzon
- Matthias Erb
- DOI
- 10.7554/elife.68642
- eISSN
- 2050-084X
- Externe Identifier
- PubMed Identifier: 34632981
- PubMed Central ID: PMC8504966
- Funding acknowledgements
- Swiss National Science Foundation: 153517
- FP7 Research and Technological Development of the European Union: FP7 MC-CIG 629134
- Schweizerischer Nationalfonds: 153517
- Deutsche Forschungsgemeinschaft: 422213951
- Swiss National Science Foundation: 189071
- Swiss National Science Foundation: 310030
- Swiss National Science Foundation: 186770
- Schweizerischer Nationalfonds: 310030_189071
- Swiss National Science Foundation: P400PB_186770
- Open access
- true
- ISSN
- 2050-084X
- Zeitschrift
- eLife
- Schlüsselwörter
- Animals
- Taraxacum
- Sesquiterpenes
- Lactones
- beta-Galactosidase
- Glucosides
- Glutathione
- Insect Proteins
- Hydrolysis
- Larva
- Digestion
- Herbivory
- Secondary Metabolism
- Inactivation, Metabolic
- Coleoptera
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2021
- Open access status
- Open Access
- Paginierung
- e68642
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2021
- Titel
- A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 10
Files
https://europepmc.org/articles/PMC8504966?pdf=render
Data source: Europe PubMed Central
- Abstract
- Gut enzymes can metabolize plant defense compounds and thereby affect the growth and fitness of insect herbivores. Whether these enzymes also influence feeding preference is largely unknown. We studied the metabolization of taraxinic acid β-D-glucopyranosyl ester (TA-G), a sesquiterpene lactone of the common dandelion (Taraxacum officinale) that deters its major root herbivore, the common cockchafer larva (Melolontha melolontha). We have demonstrated that TA-G is rapidly deglucosylated and conjugated to glutathione in the insect gut. A broad-spectrum M. melolontha β-glucosidase, Mm_bGlc17, is sufficient and necessary for TA-G deglucosylation. Using cross-species RNA interference, we have shown that Mm_bGlc17 reduces TA-G toxicity. Furthermore, Mm_bGlc17 is required for the preference of M. melolontha larvae for TA-G-deficient plants. Thus, herbivore metabolism modulates both the toxicity and deterrence of a plant defense compound. Our work illustrates the multifaceted roles of insect digestive enzymes as mediators of plant-herbivore interactions.
- Date of acceptance
- 2021
- Autoren
- Meret Huber
- Thomas Roder
- Sandra Irmisch
- Alexander Riedel
- Saskia Gablenz
- Julia Fricke
- Peter Rahfeld
- Michael Reichelt
- Christian Paetz
- Nicole Liechti
- Lingfei Hu
- Zoe Bont
- Ye Meng
- Wei Huang
- Christelle Am Robert
- Jonathan Gershenzon
- Matthias Erb
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/34632981
- DOI
- 10.7554/eLife.68642
- eISSN
- 2050-084X
- Externe Identifier
- PubMed Central ID: PMC8504966
- Zeitschrift
- Elife
- Schlüsselwörter
- Melolontha melolontha
- Taraxacum officinale
- ecology
- plant defense
- root herbivore
- sesquiterpene lactone
- β-glucosidase
- Animals
- Coleoptera
- Digestion
- Glucosides
- Glutathione
- Herbivory
- Hydrolysis
- Inactivation, Metabolic
- Insect Proteins
- Lactones
- Larva
- Secondary Metabolism
- Sesquiterpenes
- Taraxacum
- beta-Galactosidase
- Sprache
- eng
- Country
- England
- PII
- 68642
- Datum der Veröffentlichung
- 2021
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 10
Data source: PubMed
- Beziehungen:
- Property of