Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identifi cation of Cell Wall Active Antibacterial Compounds

Publication type:
Chapter
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000400473200008&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1007/978-1-4939-6634-9_7
ISBN-13
978-1-4939-6632-5
Schlüsselwörter
  • Bio-assay
  • Reporter gene
  • Cell envelope stress
  • Cell wall
  • Antibiotic
  • Antimicrobial peptide
  • Stress response
  • Luminescence
  • Lipid II cycle
Paginierung
121 - 131
Buchtitel
ANTIBIOTICS: METHODS AND PROTOCOLS
Datum der Veröffentlichung
2017
Status
Published
Titel
Application of a <i>Bacillus subtilis</i> Whole-Cell Biosensor (<i>PliaI</i>-<i>lux</i>) for the Identifi cation of Cell Wall Active Antibacterial Compounds
Ausgabe der Zeitschrift
1520

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
DOI
10.1007/978-1-4939-6634-9_7
ISBN-13
9781493966325
Online publication date
2016
Paginierung
121 - 131
Buchtitel
Methods in Molecular Biology
Datum der Veröffentlichung
2017
Status
Published
Herausgeber
Springer New York
Herausgeber URL
http://dx.doi.org/10.1007/978-1-4939-6634-9_7
Datum der Datenerfassung
2019
Titel
Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds

Data source: Crossref

Abstract
Whole-cell biosensors, based on the visualization of a reporter strain's response to a particular stimulus, are a robust and cost-effective means to monitor defined environmental conditions or the presence of chemical compounds. One specific field in which such biosensors are frequently applied is drug discovery, i.e., the screening of large numbers of bacterial or fungal strains for the production of antimicrobial compounds. We here describe the application of a luminescence-based Bacillus subtilis biosensor for the discovery of cell wall active substances. The system is based on the well-characterized promoter P <sub>liaI</sub> , which is induced in response to a wide range of conditions that cause cell envelope stress, particularly antibiotics that interfere with the membrane-anchored steps of cell wall biosynthesis. A simple "spot-on-lawn" assay, where colonies of potential producer strains are grown directly on a lawn of the reporter strain, allows for quantitative and time-resolved detection of antimicrobial compounds. Due to the very low technical demands of this procedure, we expect it to be easily applicable to a large variety of candidate producer strains and growth conditions.
Addresses
  • Department of Biology and Biochemistry, Milner Centre for Evolution, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
Autoren
DOI
10.1007/978-1-4939-6634-9_7
Open access
false
Schlüsselwörter
  • Cell Wall
  • Bacillus subtilis
  • Anti-Bacterial Agents
  • Biosensing Techniques
  • Genes, Reporter
  • Time Factors
  • Luminescence
Medium
Print
Paginierung
121 - 131
Datum der Veröffentlichung
2017
Status
Published
Datum der Datenerfassung
2016
Titel
Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds.
Ausgabe der Zeitschrift
1520

Data source: Europe PubMed Central

Abstract
Whole-cell biosensors, based on the visualization of a reporter strain's response to a particular stimulus, are a robust and cost-effective means to monitor defined environmental conditions or the presence of chemical compounds. One specific field in which such biosensors are frequently applied is drug discovery, i.e., the screening of large numbers of bacterial or fungal strains for the production of antimicrobial compounds. We here describe the application of a luminescence-based Bacillus subtilis biosensor for the discovery of cell wall active substances. The system is based on the well-characterized promoter P liaI , which is induced in response to a wide range of conditions that cause cell envelope stress, particularly antibiotics that interfere with the membrane-anchored steps of cell wall biosynthesis. A simple "spot-on-lawn" assay, where colonies of potential producer strains are grown directly on a lawn of the reporter strain, allows for quantitative and time-resolved detection of antimicrobial compounds. Due to the very low technical demands of this procedure, we expect it to be easily applicable to a large variety of candidate producer strains and growth conditions.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/27873249
DOI
10.1007/978-1-4939-6634-9_7
Schlüsselwörter
  • Antibiotic
  • Antimicrobial peptide
  • Bio-assay
  • Cell envelope stress
  • Cell wall
  • Lipid II cycle
  • Luminescence
  • Reporter gene
  • Stress response
  • Anti-Bacterial Agents
  • Bacillus subtilis
  • Biosensing Techniques
  • Cell Wall
  • Genes, Reporter
  • Luminescence
  • Time Factors
Paginierung
121 - 131
Datum der Veröffentlichung
2017
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2018
Titel
Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds.
Ausgabe der Zeitschrift
1520

Data source: PubMed

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