Biochemical characterization of an active pyrophosphate-dependent phosphofructokinase from Treponema pallidum
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- RS Roberson
- RS Ronimus
- S Gephard
- HW Morgan
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000166705500024&DestLinkType=FullRecord&DestApp=WOS_CPL
- Externe Identifier
- Clarivate Analytics Document Solution ID: 397QC
- PubMed Identifier: 11164318
- ISSN
- 0378-1097
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- FEMS MICROBIOLOGY LETTERS
- Schlüsselwörter
- syphilis
- phosphofructokinase
- glycolysis
- pyrophosphate
- Treponema pallidum
- Paginierung
- 257 - 260
- Datum der Veröffentlichung
- 2001
- Status
- Published
- Titel
- Biochemical characterization of an active pyrophosphate-dependent phosphofructokinase from <i>Treponema pallidum</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 194
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Russell S Roberson
- Ron S Ronimus
- Susanne Gephard
- Hugh W Morgan
- DOI
- 10.1111/j.1574-6968.2001.tb09479.x
- eISSN
- 1574-6968
- ISSN
- 0378-1097
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- FEMS Microbiology Letters
- Sprache
- en
- Paginierung
- 257 - 260
- Datum der Veröffentlichung
- 2001
- Status
- Published
- Herausgeber
- Oxford University Press (OUP)
- Herausgeber URL
- http://dx.doi.org/10.1111/j.1574-6968.2001.tb09479.x
- Datum der Datenerfassung
- 2019
- Titel
- Biochemical characterization of an active pyrophosphate-dependent phosphofructokinase from<i>Treponema pallidum</i>
- Ausgabe der Zeitschrift
- 194
Data source: Crossref
- Abstract
- An active pyrophosphate-dependent phosphofructokinase containing a six residue polyhistidine tag has been cloned from Treponema pallidum, and characterized biochemically. The phosphofructokinase has pH optima for activity of 8.0 for both the forward and reverse reactions. The apparent K(m) for pyrophosphate was 0.042 mM (V(max) of 141 U mg(-1) protein) and for fructose-6-phosphate, 0.529 mM. The apparent K(m) for the reverse reaction for fructose-1,6-diphosphate was 0.267 mM (V(max) of 42.4 U mg(-1) protein). The enzyme appears to be both a dimer and non-allosteric.
- Addresses
- Thermophile Research Unit, University of Waikato, Private Bag 3105, Hamilton, New Zealand.
- Autoren
- RS Roberson
- RS Ronimus
- S Gephard
- HW Morgan
- DOI
- 10.1111/j.1574-6968.2001.tb09479.x
- eISSN
- 1574-6968
- Externe Identifier
- PubMed Identifier: 11164318
- Open access
- false
- ISSN
- 0378-1097
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- FEMS microbiology letters
- Schlüsselwörter
- Treponema pallidum
- Phosphotransferases
- Cloning, Molecular
- Binding, Competitive
- Kinetics
- Molecular Weight
- Sprache
- eng
- Medium
- Paginierung
- 257 - 260
- Datum der Veröffentlichung
- 2001
- Status
- Published
- Datum der Datenerfassung
- 2001
- Titel
- Biochemical characterization of an active pyrophosphate-dependent phosphofructokinase from Treponema pallidum.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 194
Data source: Europe PubMed Central
- Abstract
- An active pyrophosphate-dependent phosphofructokinase containing a six residue polyhistidine tag has been cloned from Treponema pallidum, and characterized biochemically. The phosphofructokinase has pH optima for activity of 8.0 for both the forward and reverse reactions. The apparent K(m) for pyrophosphate was 0.042 mM (V(max) of 141 U mg(-1) protein) and for fructose-6-phosphate, 0.529 mM. The apparent K(m) for the reverse reaction for fructose-1,6-diphosphate was 0.267 mM (V(max) of 42.4 U mg(-1) protein). The enzyme appears to be both a dimer and non-allosteric.
- Autoren
- RS Roberson
- RS Ronimus
- S Gephard
- HW Morgan
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/11164318
- DOI
- 10.1111/j.1574-6968.2001.tb09479.x
- ISSN
- 0378-1097
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- FEMS Microbiol Lett
- Schlüsselwörter
- Binding, Competitive
- Cloning, Molecular
- Kinetics
- Molecular Weight
- Phosphotransferases
- Treponema pallidum
- Sprache
- eng
- Country
- England
- Paginierung
- 257 - 260
- PII
- S0378-1097(00)00518-8
- Datum der Veröffentlichung
- 2001
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2001
- Titel
- Biochemical characterization of an active pyrophosphate-dependent phosphofructokinase from Treponema pallidum.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 194
Data source: PubMed
- Beziehungen:
- Property of