Genetic optimisation of bacteria-induced calcite precipitation in Bacillus subtilis

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000720416500002&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1186/s12934-021-01704-1
eISSN
1475-2859
Externe Identifier
  • Clarivate Analytics Document Solution ID: XA1LA
  • PubMed Identifier: 34794448
Ausgabe der Veröffentlichung
1
Zeitschrift
MICROBIAL CELL FACTORIES
Schlüsselwörter
  • Microbially induced calcite precipitation
  • MICP
  • Calcite
  • Biomineralisation
  • Urease
  • Biofilm
  • Synthetic biology
  • Genetic engineering
Artikelnummer
ARTN 214
Datum der Veröffentlichung
2021
Status
Published
Titel
Genetic optimisation of bacteria-induced calcite precipitation in <i>Bacillus subtilis</i>
Sub types
  • Article
Ausgabe der Zeitschrift
20

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Microbially induced calcite precipitation (MICP) is an ancient property of bacteria, which has recently gained considerable attention for biotechnological applications. It occurs as a by-product of bacterial metabolism and involves a combination of chemical changes in the extracellular environment, e.g. pH increase, and presence of nucleation sites on the cell surface or extracellular substances produced by the bacteria. However, the molecular mechanisms underpinning MICP and the interplay between the contributing factors remain poorly understood, thus placing barriers to the full biotechnological and synthetic biology exploitation of bacterial biomineralisation.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>In this study, we adopted a bottom-up approach of systematically engineering <jats:italic>Bacillus subtilis</jats:italic>, which has no detectable intrinsic MICP activity, for biomineralisation. We showed that heterologous production of urease can induce MICP by local increases in extracellular pH, and this can be enhanced by co-expression of urease accessory genes for urea and nickel uptake, depending on environmental conditions. MICP can be strongly enhanced by biofilm-promoting conditions, which appeared to be mainly driven by production of exopolysaccharide, while the protein component of the biofilm matrix was dispensable. Attempts to modulate the cell surface charge of <jats:italic>B. subtilis</jats:italic> had surprisingly minor effects, and our results suggest this organism may intrinsically have a very negative cell surface, potentially predisposing it for MICP activity.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> <jats:p>Our findings give insights into the molecular mechanisms driving MICP in an application-relevant chassis organism and the genetic elements that can be used to engineer de novo or enhanced biomineralisation. This study also highlights mutual influences between the genetic drivers and the chemical composition of the surrounding environment in determining the speed, spatial distribution and resulting mineral crystals of MICP. Taken together, these data pave the way for future rational design of synthetic precipitator strains optimised for specific applications.</jats:p> </jats:sec>
Autoren
DOI
10.1186/s12934-021-01704-1
eISSN
1475-2859
Ausgabe der Veröffentlichung
1
Zeitschrift
Microbial Cell Factories
Sprache
en
Artikelnummer
214
Online publication date
2021
Datum der Veröffentlichung
2021
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1186/s12934-021-01704-1
Datum der Datenerfassung
2022
Titel
Genetic optimisation of bacteria-induced calcite precipitation in Bacillus subtilis
Ausgabe der Zeitschrift
20

Data source: Crossref

Abstract
<h4>Background</h4>Microbially induced calcite precipitation (MICP) is an ancient property of bacteria, which has recently gained considerable attention for biotechnological applications. It occurs as a by-product of bacterial metabolism and involves a combination of chemical changes in the extracellular environment, e.g. pH increase, and presence of nucleation sites on the cell surface or extracellular substances produced by the bacteria. However, the molecular mechanisms underpinning MICP and the interplay between the contributing factors remain poorly understood, thus placing barriers to the full biotechnological and synthetic biology exploitation of bacterial biomineralisation.<h4>Results</h4>In this study, we adopted a bottom-up approach of systematically engineering Bacillus subtilis, which has no detectable intrinsic MICP activity, for biomineralisation. We showed that heterologous production of urease can induce MICP by local increases in extracellular pH, and this can be enhanced by co-expression of urease accessory genes for urea and nickel uptake, depending on environmental conditions. MICP can be strongly enhanced by biofilm-promoting conditions, which appeared to be mainly driven by production of exopolysaccharide, while the protein component of the biofilm matrix was dispensable. Attempts to modulate the cell surface charge of B. subtilis had surprisingly minor effects, and our results suggest this organism may intrinsically have a very negative cell surface, potentially predisposing it for MICP activity.<h4>Conclusions</h4>Our findings give insights into the molecular mechanisms driving MICP in an application-relevant chassis organism and the genetic elements that can be used to engineer de novo or enhanced biomineralisation. This study also highlights mutual influences between the genetic drivers and the chemical composition of the surrounding environment in determining the speed, spatial distribution and resulting mineral crystals of MICP. Taken together, these data pave the way for future rational design of synthetic precipitator strains optimised for specific applications.
Addresses
  • Department of Biology and Biochemistry, Milner Centre for Evolution, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
Autoren
DOI
10.1186/s12934-021-01704-1
eISSN
1475-2859
Externe Identifier
  • PubMed Identifier: 34794448
  • PubMed Central ID: PMC8600894
Funding acknowledgements
  • Engineering and Physical Sciences Research Council: EP/PO2081X/1
  • Engineering and Physical Sciences Research Council: EP/P02081X/1
Open access
true
ISSN
1475-2859
Ausgabe der Veröffentlichung
1
Zeitschrift
Microbial cell factories
Schlüsselwörter
  • Biofilms
  • Bacillus subtilis
  • Calcium Carbonate
  • Nickel
  • Urea
  • Urease
  • Polysaccharides, Bacterial
  • DNA, Bacterial
  • Genetic Engineering
  • Industrial Microbiology
  • Sequence Deletion
  • Hydrogen-Ion Concentration
  • Chemical Precipitation
  • Biomineralization
Sprache
eng
Medium
Electronic
Online publication date
2021
Open access status
Open Access
Paginierung
214
Datum der Veröffentlichung
2021
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2021
Titel
Genetic optimisation of bacteria-induced calcite precipitation in Bacillus subtilis.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
20

Files

https://microbialcellfactories.biomedcentral.com/track/pdf/10.1186/s12934-021-01704-1 https://europepmc.org/articles/PMC8600894?pdf=render

Data source: Europe PubMed Central

Abstract
BACKGROUND: Microbially induced calcite precipitation (MICP) is an ancient property of bacteria, which has recently gained considerable attention for biotechnological applications. It occurs as a by-product of bacterial metabolism and involves a combination of chemical changes in the extracellular environment, e.g. pH increase, and presence of nucleation sites on the cell surface or extracellular substances produced by the bacteria. However, the molecular mechanisms underpinning MICP and the interplay between the contributing factors remain poorly understood, thus placing barriers to the full biotechnological and synthetic biology exploitation of bacterial biomineralisation. RESULTS: In this study, we adopted a bottom-up approach of systematically engineering Bacillus subtilis, which has no detectable intrinsic MICP activity, for biomineralisation. We showed that heterologous production of urease can induce MICP by local increases in extracellular pH, and this can be enhanced by co-expression of urease accessory genes for urea and nickel uptake, depending on environmental conditions. MICP can be strongly enhanced by biofilm-promoting conditions, which appeared to be mainly driven by production of exopolysaccharide, while the protein component of the biofilm matrix was dispensable. Attempts to modulate the cell surface charge of B. subtilis had surprisingly minor effects, and our results suggest this organism may intrinsically have a very negative cell surface, potentially predisposing it for MICP activity. CONCLUSIONS: Our findings give insights into the molecular mechanisms driving MICP in an application-relevant chassis organism and the genetic elements that can be used to engineer de novo or enhanced biomineralisation. This study also highlights mutual influences between the genetic drivers and the chemical composition of the surrounding environment in determining the speed, spatial distribution and resulting mineral crystals of MICP. Taken together, these data pave the way for future rational design of synthetic precipitator strains optimised for specific applications.
Date of acceptance
2021
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/34794448
DOI
10.1186/s12934-021-01704-1
eISSN
1475-2859
Externe Identifier
  • PubMed Central ID: PMC8600894
Funding acknowledgements
  • Engineering and Physical Sciences Research Council: EP/PO2081X/1
Ausgabe der Veröffentlichung
1
Zeitschrift
Microb Cell Fact
Schlüsselwörter
  • Biofilm
  • Biomineralisation
  • Calcite
  • Genetic engineering
  • MICP
  • Microbially induced calcite precipitation
  • Synthetic biology
  • Urease
  • Bacillus subtilis
  • Biofilms
  • Biomineralization
  • Calcium Carbonate
  • Chemical Precipitation
  • DNA, Bacterial
  • Genetic Engineering
  • Hydrogen-Ion Concentration
  • Industrial Microbiology
  • Nickel
  • Polysaccharides, Bacterial
  • Sequence Deletion
  • Urea
  • Urease
Sprache
eng
Country
England
Paginierung
214
PII
10.1186/s12934-021-01704-1
Datum der Veröffentlichung
2021
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Titel
Genetic optimisation of bacteria-induced calcite precipitation in Bacillus subtilis.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
20

Data source: PubMed

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