Identification and Characterization of a Bacitracin Resistance Network in Enterococcus faecalis
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Susanne Gebhard
- Chong Fang
- Aishath Shaaly
- David J Leslie
- Marion R Weimar
- Falk Kalamorz
- Alan Carne
- Gregory M Cook
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000332175100021&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1128/AAC.02111-13
- eISSN
- 1098-6596
- Externe Identifier
- Clarivate Analytics Document Solution ID: AC0HJ
- PubMed Identifier: 24342648
- ISSN
- 0066-4804
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
- Paginierung
- 1425 - 1433
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Titel
- Identification and Characterization of a Bacitracin Resistance Network in <i>Enterococcus faecalis</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 58
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>ABSTRACT</jats:title> <jats:p> Resistance of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Enterococcus faecalis</jats:named-content> against antimicrobial peptides, both of host origin and produced by other bacteria of the gut microflora, is likely to be an important factor in the bacterium's success as an intestinal commensal. The aim of this study was to identify proteins with a role in resistance against the model antimicrobial peptide bacitracin. Proteome analysis of bacitracin-treated and untreated cells showed that bacitracin stress induced the expression of cell wall-biosynthetic proteins and caused metabolic rearrangements. Among the proteins with increased production, an ATP-binding cassette (ABC) transporter with similarity to known peptide antibiotic resistance systems was identified and shown to mediate resistance against bacitracin. Expression of the transporter was dependent on a two-component regulatory system and a second ABC transporter, which were identified by genome analysis. Both resistance and the regulatory pathway could be functionally transferred to <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Bacillus subtilis</jats:named-content> , proving the function and sufficiency of these components for bacitracin resistance. Our data therefore show that the two ABC transporters and the two-component system form a resistance network against antimicrobial peptides in <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. faecalis</jats:named-content> , where one transporter acts as the sensor that activates the TCS to induce production of the second transporter, which mediates the actual resistance. </jats:p>
- Autoren
- Susanne Gebhard
- Chong Fang
- Aishath Shaaly
- David J Leslie
- Marion R Weimar
- Falk Kalamorz
- Alan Carne
- Gregory M Cook
- DOI
- 10.1128/aac.02111-13
- eISSN
- 1098-6596
- ISSN
- 0066-4804
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Antimicrobial Agents and Chemotherapy
- Sprache
- en
- Paginierung
- 1425 - 1433
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Herausgeber
- American Society for Microbiology
- Herausgeber URL
- http://dx.doi.org/10.1128/aac.02111-13
- Datum der Datenerfassung
- 2022
- Titel
- Identification and Characterization of a Bacitracin Resistance Network in Enterococcus faecalis
- Ausgabe der Zeitschrift
- 58
Data source: Crossref
- Abstract
- Resistance of Enterococcus faecalis against antimicrobial peptides, both of host origin and produced by other bacteria of the gut microflora, is likely to be an important factor in the bacterium's success as an intestinal commensal. The aim of this study was to identify proteins with a role in resistance against the model antimicrobial peptide bacitracin. Proteome analysis of bacitracin-treated and untreated cells showed that bacitracin stress induced the expression of cell wall-biosynthetic proteins and caused metabolic rearrangements. Among the proteins with increased production, an ATP-binding cassette (ABC) transporter with similarity to known peptide antibiotic resistance systems was identified and shown to mediate resistance against bacitracin. Expression of the transporter was dependent on a two-component regulatory system and a second ABC transporter, which were identified by genome analysis. Both resistance and the regulatory pathway could be functionally transferred to Bacillus subtilis, proving the function and sufficiency of these components for bacitracin resistance. Our data therefore show that the two ABC transporters and the two-component system form a resistance network against antimicrobial peptides in E. faecalis, where one transporter acts as the sensor that activates the TCS to induce production of the second transporter, which mediates the actual resistance.
- Addresses
- Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Martinsried, Germany.
- Autoren
- Susanne Gebhard
- Chong Fang
- Aishath Shaaly
- David J Leslie
- Marion R Weimar
- Falk Kalamorz
- Alan Carne
- Gregory M Cook
- DOI
- 10.1128/aac.02111-13
- eISSN
- 1098-6596
- Externe Identifier
- PubMed Identifier: 24342648
- PubMed Central ID: PMC3957847
- Open access
- false
- ISSN
- 0066-4804
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Antimicrobial agents and chemotherapy
- Schlüsselwörter
- Enterococcus faecalis
- Bacitracin
- ATP-Binding Cassette Transporters
- Proteome
- Anti-Bacterial Agents
- Microbial Sensitivity Tests
- Drug Resistance, Bacterial
- Gene Expression Regulation, Bacterial
- Promoter Regions, Genetic
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2013
- Paginierung
- 1425 - 1433
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum der Datenerfassung
- 2013
- Titel
- Identification and characterization of a bacitracin resistance network in Enterococcus faecalis.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 58
Files
https://aac.asm.org/content/aac/58/3/1425.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24342648/pdf/?tool=EBI https://europepmc.org/articles/PMC3957847?pdf=render
Data source: Europe PubMed Central
- Abstract
- Resistance of Enterococcus faecalis against antimicrobial peptides, both of host origin and produced by other bacteria of the gut microflora, is likely to be an important factor in the bacterium's success as an intestinal commensal. The aim of this study was to identify proteins with a role in resistance against the model antimicrobial peptide bacitracin. Proteome analysis of bacitracin-treated and untreated cells showed that bacitracin stress induced the expression of cell wall-biosynthetic proteins and caused metabolic rearrangements. Among the proteins with increased production, an ATP-binding cassette (ABC) transporter with similarity to known peptide antibiotic resistance systems was identified and shown to mediate resistance against bacitracin. Expression of the transporter was dependent on a two-component regulatory system and a second ABC transporter, which were identified by genome analysis. Both resistance and the regulatory pathway could be functionally transferred to Bacillus subtilis, proving the function and sufficiency of these components for bacitracin resistance. Our data therefore show that the two ABC transporters and the two-component system form a resistance network against antimicrobial peptides in E. faecalis, where one transporter acts as the sensor that activates the TCS to induce production of the second transporter, which mediates the actual resistance.
- Autoren
- Susanne Gebhard
- Chong Fang
- Aishath Shaaly
- David J Leslie
- Marion R Weimar
- Falk Kalamorz
- Alan Carne
- Gregory M Cook
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/24342648
- DOI
- 10.1128/AAC.02111-13
- eISSN
- 1098-6596
- Externe Identifier
- PubMed Central ID: PMC3957847
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Antimicrob Agents Chemother
- Schlüsselwörter
- ATP-Binding Cassette Transporters
- Anti-Bacterial Agents
- Bacitracin
- Drug Resistance, Bacterial
- Enterococcus faecalis
- Gene Expression Regulation, Bacterial
- Microbial Sensitivity Tests
- Promoter Regions, Genetic
- Proteome
- Sprache
- eng
- Country
- United States
- Paginierung
- 1425 - 1433
- PII
- AAC.02111-13
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2014
- Titel
- Identification and characterization of a bacitracin resistance network in Enterococcus faecalis.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 58
Data source: PubMed
- Beziehungen:
- Property of