The SigF Regulon in Mycobacterium smegmatis Reveals Roles in Adaptation to Stationary Phase, Heat, and Oxidative Stress

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000277161400003&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1128/JB.00035-10
eISSN
1098-5530
Externe Identifier
  • Clarivate Analytics Document Solution ID: 589OO
  • PubMed Identifier: 20233930
ISSN
0021-9193
Ausgabe der Veröffentlichung
10
Zeitschrift
JOURNAL OF BACTERIOLOGY
Paginierung
2491 - 2502
Datum der Veröffentlichung
2010
Status
Published
Titel
The SigF Regulon in <i>Mycobacterium smegmatis</i> Reveals Roles in Adaptation to Stationary Phase, Heat, and Oxidative Stress
Sub types
  • Article
Ausgabe der Zeitschrift
192

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>ABSTRACT</jats:title> <jats:p> SigF is an alternative sigma factor that is highly conserved among species of the genus <jats:italic>Mycobacterium</jats:italic> . In this study we identified the SigF regulon in <jats:italic>Mycobacterium smegmatis</jats:italic> using whole-genome microarray and promoter consensus analyses. In total, 64 genes in exponential phase and 124 genes in stationary phase are SigF dependent ( <jats:italic>P</jats:italic> &lt; 0.01, &gt;2-fold expression change). Our experimental data reveal the SigF-dependent promoter consensus GTTT-N <jats:sub>(15-17)</jats:sub> -GGGTA for <jats:italic>M. smegmatis</jats:italic> , and we propose 130 potential genes under direct control of SigF, of which more than 50% exhibited reduced expression in a Δ <jats:italic>sigF</jats:italic> strain. We previously reported an increased susceptibility of the Δ <jats:italic>sigF</jats:italic> strain to heat and oxidative stress, and our expression data indicate a molecular basis for these phenotypes. We observed SigF-dependent expression of several genes purportedly involved in oxidative stress defense, namely, a heme-containing catalase, a manganese-containing catalase, a superoxide dismutase, the starvation-induced DNA-protecting protein MsDps1, and the biosynthesis genes for the carotenoid isorenieratene. Our data suggest that SigF regulates the biosynthesis of the thermoprotectant trehalose, as well as an uptake system for osmoregulatory compounds, and this may explain the increased heat susceptibility of the Δ <jats:italic>sigF</jats:italic> strain. We identified the regulatory proteins SigH3, PhoP, WhiB1, and WhiB4 as possible genes under direct control of SigF and propose four novel anti-sigma factor antagonists that could be involved in the posttranslational regulation of SigF in <jats:italic>M. smegmatis</jats:italic> . This study emphasizes the importance of this sigma factor for stationary-phase adaptation and stress response in mycobacteria. </jats:p>
Autoren
DOI
10.1128/jb.00035-10
eISSN
1098-5530
ISSN
0021-9193
Ausgabe der Veröffentlichung
10
Zeitschrift
Journal of Bacteriology
Sprache
en
Paginierung
2491 - 2502
Datum der Veröffentlichung
2010
Status
Published
Herausgeber
American Society for Microbiology
Herausgeber URL
http://dx.doi.org/10.1128/jb.00035-10
Datum der Datenerfassung
2021
Titel
The SigF Regulon in <i>Mycobacterium smegmatis</i> Reveals Roles in Adaptation to Stationary Phase, Heat, and Oxidative Stress
Ausgabe der Zeitschrift
192

Data source: Crossref

Abstract
SigF is an alternative sigma factor that is highly conserved among species of the genus Mycobacterium. In this study we identified the SigF regulon in Mycobacterium smegmatis using whole-genome microarray and promoter consensus analyses. In total, 64 genes in exponential phase and 124 genes in stationary phase are SigF dependent (P < 0.01, >2-fold expression change). Our experimental data reveal the SigF-dependent promoter consensus GTTT-N((15-17))-GGGTA for M. smegmatis, and we propose 130 potential genes under direct control of SigF, of which more than 50% exhibited reduced expression in a Delta sigF strain. We previously reported an increased susceptibility of the Delta sigF strain to heat and oxidative stress, and our expression data indicate a molecular basis for these phenotypes. We observed SigF-dependent expression of several genes purportedly involved in oxidative stress defense, namely, a heme-containing catalase, a manganese-containing catalase, a superoxide dismutase, the starvation-induced DNA-protecting protein MsDps1, and the biosynthesis genes for the carotenoid isorenieratene. Our data suggest that SigF regulates the biosynthesis of the thermoprotectant trehalose, as well as an uptake system for osmoregulatory compounds, and this may explain the increased heat susceptibility of the Delta sigF strain. We identified the regulatory proteins SigH3, PhoP, WhiB1, and WhiB4 as possible genes under direct control of SigF and propose four novel anti-sigma factor antagonists that could be involved in the posttranslational regulation of SigF in M. smegmatis. This study emphasizes the importance of this sigma factor for stationary-phase adaptation and stress response in mycobacteria.
Addresses
  • Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, P.O. Box 56, Dunedin, New Zealand.
Autoren
DOI
10.1128/jb.00035-10
eISSN
1098-5530
Externe Identifier
  • PubMed Identifier: 20233930
  • PubMed Central ID: PMC2863567
Open access
false
ISSN
0021-9193
Ausgabe der Veröffentlichung
10
Zeitschrift
Journal of bacteriology
Schlüsselwörter
  • Mycobacterium smegmatis
  • Bacterial Proteins
  • Sigma Factor
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction
  • Gene Expression Regulation, Bacterial
  • Oxidative Stress
  • Regulon
  • Hot Temperature
  • Promoter Regions, Genetic
Sprache
eng
Medium
Print-Electronic
Online publication date
2010
Paginierung
2491 - 2502
Datum der Veröffentlichung
2010
Status
Published
Datum der Datenerfassung
2010
Titel
The SigF regulon in Mycobacterium smegmatis reveals roles in adaptation to stationary phase, heat, and oxidative stress.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
  • Research Support, N.I.H., Extramural
Ausgabe der Zeitschrift
192

Files

https://jb.asm.org/content/192/10/2491.full.pdf http://jb.asm.org/cgi/reprint/192/10/2491 https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20233930/pdf/?tool=EBI https://europepmc.org/articles/PMC2863567?pdf=render

Data source: Europe PubMed Central

Abstract
SigF is an alternative sigma factor that is highly conserved among species of the genus Mycobacterium. In this study we identified the SigF regulon in Mycobacterium smegmatis using whole-genome microarray and promoter consensus analyses. In total, 64 genes in exponential phase and 124 genes in stationary phase are SigF dependent (P < 0.01, >2-fold expression change). Our experimental data reveal the SigF-dependent promoter consensus GTTT-N((15-17))-GGGTA for M. smegmatis, and we propose 130 potential genes under direct control of SigF, of which more than 50% exhibited reduced expression in a Delta sigF strain. We previously reported an increased susceptibility of the Delta sigF strain to heat and oxidative stress, and our expression data indicate a molecular basis for these phenotypes. We observed SigF-dependent expression of several genes purportedly involved in oxidative stress defense, namely, a heme-containing catalase, a manganese-containing catalase, a superoxide dismutase, the starvation-induced DNA-protecting protein MsDps1, and the biosynthesis genes for the carotenoid isorenieratene. Our data suggest that SigF regulates the biosynthesis of the thermoprotectant trehalose, as well as an uptake system for osmoregulatory compounds, and this may explain the increased heat susceptibility of the Delta sigF strain. We identified the regulatory proteins SigH3, PhoP, WhiB1, and WhiB4 as possible genes under direct control of SigF and propose four novel anti-sigma factor antagonists that could be involved in the posttranslational regulation of SigF in M. smegmatis. This study emphasizes the importance of this sigma factor for stationary-phase adaptation and stress response in mycobacteria.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20233930
DOI
10.1128/JB.00035-10
eISSN
1098-5530
Externe Identifier
  • PubMed Central ID: PMC2863567
Ausgabe der Veröffentlichung
10
Zeitschrift
J Bacteriol
Schlüsselwörter
  • Bacterial Proteins
  • Gene Expression Regulation, Bacterial
  • Hot Temperature
  • Mycobacterium smegmatis
  • Oligonucleotide Array Sequence Analysis
  • Oxidative Stress
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Regulon
  • Sigma Factor
Sprache
eng
Country
United States
Paginierung
2491 - 2502
PII
JB.00035-10
Datum der Veröffentlichung
2010
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
The SigF regulon in Mycobacterium smegmatis reveals roles in adaptation to stationary phase, heat, and oxidative stress.
Sub types
  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
192

Data source: PubMed

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