Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production

Autoren

Judit Willenbacher
Teresa Mohr
Marius Henkel
Susanne Gebhard
Thorsten Mascher
Christoph Syldatk
Rudolf Hausmann

DOI

10.1016/j.jbiotec.2016.03.002

ISSN

0168-1656

Zeitschrift

Journal of Biotechnology

Sprache

en

Paginierung

14 - 17

Datum der Veröffentlichung

2016

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/j.jbiotec.2016.03.002

Datum der Datenerfassung

2022

Titel

Substitution of the native srfA promoter by constitutive P in two B. subtilis strains and evaluation of the effect on Surfactin production

Ausgabe der Zeitschrift

224

Data source: Crossref

Abstract

The genetic enhancement of Surfactin production increasingly gained attention in the last years, since relatively low product yields limit the industrial application of this biosurfactant. The natural quorum sensing regulation of the srfA operon (coding for the Surfactin synthetase) can reasonably be assumed to be the bottleneck of Surfactin synthesis. Therefore, the replacement of the naturally quorum sensing regulated, and herewith cell density dependent, promoter PsrfA against the Bacillus subtilis endogenous and constitutive promoter Pveg was hypothesized to generally enhance Surfactin yields. The markerless promoter replacement was conducted in the two B. subtilis Surfactin producer strains 3A38 and DSM 10(T). The promoter substitution led to an enhancement of Surfactin concentrations in the producer strain 3A38, initially producing only minor amounts of Surfactin (0.07g/L increased to 0.26g/L). In contrast, promoter exchange in B. subtilis DSM 10(T) (wild-type strain producing 0.62g/L Surfactin) did not achieve an enhancement of Surfactin concentrations (detrimental reduction to 0.04g/L). These findings implicate that Surfactin synthesis is differently regulated in minor and strong Surfactin producer strains. The hypothesized general enhancement of Surfactin yields after substitution of the native promoter was therefore not confirmed.

Addresses

Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology (KIT), Engler-Bunte-Ring 3, 76131 Karlsruhe, Germany. Electronic address: judit.willenbacher@kit.edu.

Autoren

Judit Willenbacher
Teresa Mohr
Marius Henkel
Susanne Gebhard
Thorsten Mascher
Christoph Syldatk
Rudolf Hausmann

DOI

10.1016/j.jbiotec.2016.03.002

eISSN

1873-4863

Externe Identifier

PubMed Identifier: 26953743

Open access

false

ISSN

0168-1656

Zeitschrift

Journal of biotechnology

Schlüsselwörter

Bacillus subtilis
Peptides, Cyclic
Peptide Synthases
Bacterial Proteins
Recombinant Proteins
Gene Expression Regulation, Bacterial
Genes, Bacterial
Quorum Sensing
Promoter Regions, Genetic
Lipopeptides

Sprache

eng

Medium

Print-Electronic

Online publication date

2016

Paginierung

14 - 17

Datum der Veröffentlichung

2016

Status

Published

Datum der Datenerfassung

2016

Titel

Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production.

Sub types

Journal Article

Ausgabe der Zeitschrift

224

Data source: Europe PubMed Central

Abstract

The genetic enhancement of Surfactin production increasingly gained attention in the last years, since relatively low product yields limit the industrial application of this biosurfactant. The natural quorum sensing regulation of the srfA operon (coding for the Surfactin synthetase) can reasonably be assumed to be the bottleneck of Surfactin synthesis. Therefore, the replacement of the naturally quorum sensing regulated, and herewith cell density dependent, promoter PsrfA against the Bacillus subtilis endogenous and constitutive promoter Pveg was hypothesized to generally enhance Surfactin yields. The markerless promoter replacement was conducted in the two B. subtilis Surfactin producer strains 3A38 and DSM 10(T). The promoter substitution led to an enhancement of Surfactin concentrations in the producer strain 3A38, initially producing only minor amounts of Surfactin (0.07g/L increased to 0.26g/L). In contrast, promoter exchange in B. subtilis DSM 10(T) (wild-type strain producing 0.62g/L Surfactin) did not achieve an enhancement of Surfactin concentrations (detrimental reduction to 0.04g/L). These findings implicate that Surfactin synthesis is differently regulated in minor and strong Surfactin producer strains. The hypothesized general enhancement of Surfactin yields after substitution of the native promoter was therefore not confirmed.

Date of acceptance

2016

Autoren

Judit Willenbacher
Teresa Mohr
Marius Henkel
Susanne Gebhard
Thorsten Mascher
Christoph Syldatk
Rudolf Hausmann

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/26953743

DOI

10.1016/j.jbiotec.2016.03.002

eISSN

1873-4863

Zeitschrift

J Biotechnol

Schlüsselwörter

Bacillus subtilis
Biosurfactant
Promoter exchange
Quorum sensing
Surfactin
srfA operon
Bacillus subtilis
Bacterial Proteins
Gene Expression Regulation, Bacterial
Genes, Bacterial
Lipopeptides
Peptide Synthases
Peptides, Cyclic
Promoter Regions, Genetic
Quorum Sensing
Recombinant Proteins

Sprache

eng

Country

Netherlands

Paginierung

14 - 17

PII

S0168-1656(16)30100-6

Datum der Veröffentlichung

2016

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2016

Titel

Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production.

Sub types

Journal Article

Ausgabe der Zeitschrift

224

Data source: PubMed

Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production

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