Glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR) and nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN), key enzymes of the respective modified Embden-Meyerhof pathways in the hyperthermophilic crenarchaeota Pyrobaculum aerophilum and Aeropyrum pernix

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000248800600010&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1111/j.1574-6968.2007.00787.x
eISSN
1574-6968
Externe Identifier
  • Clarivate Analytics Document Solution ID: 200ZF
  • PubMed Identifier: 17559573
ISSN
0378-1097
Ausgabe der Veröffentlichung
2
Zeitschrift
FEMS MICROBIOLOGY LETTERS
Schlüsselwörter
  • Aeropyrum pernix
  • glyceraldehydes-3-phosphate ferredoxin oxidoreductase
  • modified Embden-Meyerh of pathway
  • nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase
  • Pyrobaculum aerophilum
Paginierung
196 - 205
Datum der Veröffentlichung
2007
Status
Published
Titel
Glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR) and nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN), key enzymes of the respective modified Embden-Meyerhof pathways in the hyperthermophilic crenarchaeota <i>Pyrobaculum aerophilum</i> and <i>Aeropyrum pernix</i>
Sub types
  • Article
Ausgabe der Zeitschrift
273

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
DOI
10.1111/j.1574-6968.2007.00787.x
eISSN
1574-6968
ISSN
0378-1097
Ausgabe der Veröffentlichung
2
Zeitschrift
FEMS Microbiology Letters
Sprache
en
Paginierung
196 - 205
Datum der Veröffentlichung
2007
Status
Published
Herausgeber
Oxford University Press (OUP)
Herausgeber URL
http://dx.doi.org/10.1111/j.1574-6968.2007.00787.x
Datum der Datenerfassung
2023
Titel
Glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR) and nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN), key enzymes of the respective modified Embden–Meyerhof pathways in the hyperthermophilic crenarchaeota<i>Pyrobaculum aerophilum</i>and<i>Aeropyrum pernix</i>
Ausgabe der Zeitschrift
273

Data source: Crossref

Abstract
The growth of Pyrobaculum aerophilum on yeast extract and nitrate was stimulated by the addition of maltose. Extracts of maltose/yeast extract/nitrate-grown cells contained all enzyme activities of a modified Embden-Meyerhof (EM) pathway, including ATP-dependent glucokinase, phosphoglucose isomerase, ATP-dependent 6-phosphofructokinase, fructose-1,6-phosphate aldolase, triose-phosphate isomerase, GAPOR, phosphoglycerate mutase, enolase and pyruvate kinase. The activity of GAPOR was stimulated about fourfold by maltose, indicating a role in sugar degradation. GAPOR was purified 200-fold to homogeneity and characterized as a 67 kDa monomeric, extremely thermostable protein. The enzyme showed high specificity for glyceraldehyde-3-phosphate and did not use glyceraldehyde, acetaldehyde or formaldehyde as substrates. By matrix-assisted laser desorption/ionization-time of flight analysis of the purified enzyme, ORF PA1029 was identified as a coding gene, gapor, in the sequenced genome of Pyrobaculum aerophilum. The data indicate that the (micro)aerophilic Pyrobaculum aerophilum contains a functional GAPOR as part of a modified EM pathway. Cells of the strictly aerobic crenarchaeon Aeropyrum pernix also contain enzyme activities of a modified EM pathway similar to that of Pyrobaculum aerophilum, except that a GAPN activity replaces GAPOR activity.
Addresses
  • Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität Kiel, Kiel, Germany.
Autoren
DOI
10.1111/j.1574-6968.2007.00787.x
eISSN
1574-6968
Externe Identifier
  • PubMed Identifier: 17559573
Open access
false
ISSN
0378-1097
Ausgabe der Veröffentlichung
2
Zeitschrift
FEMS microbiology letters
Schlüsselwörter
  • Aeropyrum
  • Pyrobaculum
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Phylogeny
  • Substrate Specificity
  • Glycolysis
  • Carbohydrate Metabolism
Sprache
eng
Medium
Print-Electronic
Online publication date
2007
Paginierung
196 - 205
Datum der Veröffentlichung
2007
Status
Published
Datum der Datenerfassung
2007
Titel
Glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR) and nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN), key enzymes of the respective modified Embden-Meyerhof pathways in the hyperthermophilic crenarchaeota Pyrobaculum aerophilum and Aeropyrum pernix.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
273

Data source: Europe PubMed Central

Abstract
The growth of Pyrobaculum aerophilum on yeast extract and nitrate was stimulated by the addition of maltose. Extracts of maltose/yeast extract/nitrate-grown cells contained all enzyme activities of a modified Embden-Meyerhof (EM) pathway, including ATP-dependent glucokinase, phosphoglucose isomerase, ATP-dependent 6-phosphofructokinase, fructose-1,6-phosphate aldolase, triose-phosphate isomerase, GAPOR, phosphoglycerate mutase, enolase and pyruvate kinase. The activity of GAPOR was stimulated about fourfold by maltose, indicating a role in sugar degradation. GAPOR was purified 200-fold to homogeneity and characterized as a 67 kDa monomeric, extremely thermostable protein. The enzyme showed high specificity for glyceraldehyde-3-phosphate and did not use glyceraldehyde, acetaldehyde or formaldehyde as substrates. By matrix-assisted laser desorption/ionization-time of flight analysis of the purified enzyme, ORF PA1029 was identified as a coding gene, gapor, in the sequenced genome of Pyrobaculum aerophilum. The data indicate that the (micro)aerophilic Pyrobaculum aerophilum contains a functional GAPOR as part of a modified EM pathway. Cells of the strictly aerobic crenarchaeon Aeropyrum pernix also contain enzyme activities of a modified EM pathway similar to that of Pyrobaculum aerophilum, except that a GAPN activity replaces GAPOR activity.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/17559573
DOI
10.1111/j.1574-6968.2007.00787.x
ISSN
0378-1097
Ausgabe der Veröffentlichung
2
Zeitschrift
FEMS Microbiol Lett
Schlüsselwörter
  • Aeropyrum
  • Carbohydrate Metabolism
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Glycolysis
  • Phylogeny
  • Pyrobaculum
  • Substrate Specificity
Sprache
eng
Country
England
Paginierung
196 - 205
PII
FML787
Datum der Veröffentlichung
2007
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2007
Titel
Glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR) and nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN), key enzymes of the respective modified Embden-Meyerhof pathways in the hyperthermophilic crenarchaeota Pyrobaculum aerophilum and Aeropyrum pernix.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
273

Data source: PubMed

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