Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor

Publication type:
Journal article
Metadata:
Autoren
  • Pol Besenius
  • Peter AG Cormack
  • R Frederick Ludlow
  • Sijbren Otto
  • David C Sherrington
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000277399300018&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1039/c000333f
Externe Identifier
  • Clarivate Analytics Document Solution ID: 592SE
  • PubMed Identifier: 20448900
ISSN
1477-0520
Ausgabe der Veröffentlichung
10
Zeitschrift
ORGANIC & BIOMOLECULAR CHEMISTRY
Paginierung
2414 - 2418
Datum der Veröffentlichung
2010
Status
Published
Titel
Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor
Sub types
  • Article
Ausgabe der Zeitschrift
8

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Pol Besenius
  • Peter AG Cormack
  • R Frederick Ludlow
  • Sijbren Otto
  • David C Sherrington
DOI
10.1039/c000333f
eISSN
1477-0539
ISSN
1477-0520
Ausgabe der Veröffentlichung
10
Zeitschrift
Organic & Biomolecular Chemistry
Sprache
en
Paginierung
2414 - 2414
Datum der Veröffentlichung
2010
Status
Published
Herausgeber
Royal Society of Chemistry (RSC)
Herausgeber URL
http://dx.doi.org/10.1039/c000333f
Datum der Datenerfassung
2024
Titel
Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor
Ausgabe der Zeitschrift
8

Data source: Crossref

Abstract
We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly(N,N-dimethylacrylamide)-based support, nonselective ion-exchange type behaviour between the oppositely charged cationic guest and polyanionic hosts was overcome, such that the selective molecular recognition arising in aqueous solution reactions is manifest also in the analogous templated solid phase DCL syntheses. The ability of a polymer bound template to identify and isolate a synthetic receptor via dynamic combinatorial chemistry was not compromised by the large size of the library, consisting of well over 140 theoretical members, demonstrating the practical advantages of a polymer-supported DCL methodology.
Addresses
  • WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK G1 1XL.
Autoren
  • Pol Besenius
  • Peter AG Cormack
  • R Frederick Ludlow
  • Sijbren Otto
  • David C Sherrington
DOI
10.1039/c000333f
eISSN
1477-0539
Externe Identifier
  • PubMed Identifier: 20448900
Open access
false
ISSN
1477-0520
Ausgabe der Veröffentlichung
10
Zeitschrift
Organic & biomolecular chemistry
Schlüsselwörter
  • Disulfides
  • Water
  • Acrylamides
  • Amantadine
  • Chromatography, Affinity
  • Chromatography, High Pressure Liquid
  • Combinatorial Chemistry Techniques
  • Nanostructures
Sprache
eng
Medium
Print-Electronic
Online publication date
2010
Paginierung
2414 - 2418
Datum der Veröffentlichung
2010
Status
Published
Datum der Datenerfassung
2010
Titel
Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
8

Data source: Europe PubMed Central

Abstract
We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly(N,N-dimethylacrylamide)-based support, nonselective ion-exchange type behaviour between the oppositely charged cationic guest and polyanionic hosts was overcome, such that the selective molecular recognition arising in aqueous solution reactions is manifest also in the analogous templated solid phase DCL syntheses. The ability of a polymer bound template to identify and isolate a synthetic receptor via dynamic combinatorial chemistry was not compromised by the large size of the library, consisting of well over 140 theoretical members, demonstrating the practical advantages of a polymer-supported DCL methodology.
Autoren
  • Pol Besenius
  • Peter AG Cormack
  • R Frederick Ludlow
  • Sijbren Otto
  • David C Sherrington
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20448900
DOI
10.1039/c000333f
eISSN
1477-0539
Ausgabe der Veröffentlichung
10
Zeitschrift
Org Biomol Chem
Schlüsselwörter
  • Acrylamides
  • Amantadine
  • Chromatography, Affinity
  • Chromatography, High Pressure Liquid
  • Combinatorial Chemistry Techniques
  • Disulfides
  • Nanostructures
  • Water
Sprache
eng
Country
England
Paginierung
2414 - 2418
Datum der Veröffentlichung
2010
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
8

Data source: PubMed

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