Insulation and wiring specificity of BceR-like response regulators and their target promoters in Bacillus subtilis
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Chong Fang
- Anna Nagy-Staron
- Martin Grafe
- Ralf Heermann
- Kirsten Jung
- Susanne Gebhard
- Thorsten Mascher
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000398059200002&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1111/mmi.13597
- eISSN
- 1365-2958
- Externe Identifier
- Clarivate Analytics Document Solution ID: EQ4PC
- PubMed Identifier: 27997719
- ISSN
- 0950-382X
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- MOLECULAR MICROBIOLOGY
- Paginierung
- 16 - 31
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Titel
- Insulation and wiring specificity of BceR-like response regulators and their target promoters in <i>Bacillus subtilis</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 104
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Summary</jats:title><jats:p>BceRS and PsdRS are paralogous two‐component systems in <jats:italic>Bacillus subtilis</jats:italic> controlling the response to antimicrobial peptides. In the presence of extracellular bacitracin and nisin, respectively, the two response regulators (RRs) bind their target promoters, P<jats:sub><jats:italic>bceA</jats:italic></jats:sub> or P<jats:sub><jats:italic>psdA</jats:italic></jats:sub>, resulting in a strong up‐regulation of target gene expression and ultimately antibiotic resistance. Despite high sequence similarity between the RRs BceR and PsdR and their known binding sites, no cross‐regulation has been observed between them. We therefore investigated the specificity determinants of P<jats:sub><jats:italic>bceA</jats:italic></jats:sub> and P<jats:sub><jats:italic>psdA</jats:italic></jats:sub> that ensure the insulation of these two paralogous pathways at the RR–promoter interface. <jats:italic>In vivo</jats:italic> and <jats:italic>in vitro</jats:italic> analyses demonstrate that the regulatory regions within these two promoters contain three important elements: in addition to the known (main) binding site, we identified a linker region and a secondary binding site that are crucial for functionality. Initial binding to the high‐affinity, low‐specificity main binding site is a prerequisite for the subsequent highly specific binding of a second RR dimer to the low‐affinity secondary binding site. In addition to this hierarchical cooperative binding, discrimination requires a competition of the two RRs for their respective binding site mediated by only slight differences in binding affinities.</jats:p>
- Autoren
- Chong Fang
- Anna Nagy‐Staroń
- Martin Grafe
- Ralf Heermann
- Kirsten Jung
- Susanne Gebhard
- Thorsten Mascher
- DOI
- 10.1111/mmi.13597
- eISSN
- 1365-2958
- ISSN
- 0950-382X
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Molecular Microbiology
- Sprache
- en
- Online publication date
- 2017
- Paginierung
- 16 - 31
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1111/mmi.13597
- Datum der Datenerfassung
- 2023
- Titel
- Insulation and wiring specificity of BceR‐like response regulators and their target promoters in <i>Bacillus subtilis</i>
- Ausgabe der Zeitschrift
- 104
Data source: Crossref
- Abstract
- BceRS and PsdRS are paralogous two-component systems in Bacillus subtilis controlling the response to antimicrobial peptides. In the presence of extracellular bacitracin and nisin, respectively, the two response regulators (RRs) bind their target promoters, P<sub>bceA</sub> or P<sub>psdA</sub> , resulting in a strong up-regulation of target gene expression and ultimately antibiotic resistance. Despite high sequence similarity between the RRs BceR and PsdR and their known binding sites, no cross-regulation has been observed between them. We therefore investigated the specificity determinants of P<sub>bceA</sub> and P<sub>psdA</sub> that ensure the insulation of these two paralogous pathways at the RR-promoter interface. In vivo and in vitro analyses demonstrate that the regulatory regions within these two promoters contain three important elements: in addition to the known (main) binding site, we identified a linker region and a secondary binding site that are crucial for functionality. Initial binding to the high-affinity, low-specificity main binding site is a prerequisite for the subsequent highly specific binding of a second RR dimer to the low-affinity secondary binding site. In addition to this hierarchical cooperative binding, discrimination requires a competition of the two RRs for their respective binding site mediated by only slight differences in binding affinities.
- Addresses
- Department Biology I, Ludwig-Maximilians-Universität München, Großhaderner Str. 2-4, Martinsried, 82152, Germany.
- Autoren
- Chong Fang
- Anna Nagy-Staroń
- Martin Grafe
- Ralf Heermann
- Kirsten Jung
- Susanne Gebhard
- Thorsten Mascher
- DOI
- 10.1111/mmi.13597
- eISSN
- 1365-2958
- Externe Identifier
- PubMed Identifier: 27997719
- Open access
- false
- ISSN
- 0950-382X
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Molecular microbiology
- Schlüsselwörter
- Bacillus subtilis
- Bacitracin
- Nisin
- Bacterial Proteins
- ATP-Binding Cassette Transporters
- Anti-Bacterial Agents
- Gene Expression Regulation, Bacterial
- Up-Regulation
- Binding Sites
- Base Sequence
- Regulatory Sequences, Nucleic Acid
- Protein Binding
- Molecular Sequence Data
- Promoter Regions, Genetic
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2017
- Paginierung
- 16 - 31
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Datum der Datenerfassung
- 2016
- Titel
- Insulation and wiring specificity of BceR-like response regulators and their target promoters in Bacillus subtilis.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 104
Data source: Europe PubMed Central
- Abstract
- BceRS and PsdRS are paralogous two-component systems in Bacillus subtilis controlling the response to antimicrobial peptides. In the presence of extracellular bacitracin and nisin, respectively, the two response regulators (RRs) bind their target promoters, PbceA or PpsdA , resulting in a strong up-regulation of target gene expression and ultimately antibiotic resistance. Despite high sequence similarity between the RRs BceR and PsdR and their known binding sites, no cross-regulation has been observed between them. We therefore investigated the specificity determinants of PbceA and PpsdA that ensure the insulation of these two paralogous pathways at the RR-promoter interface. In vivo and in vitro analyses demonstrate that the regulatory regions within these two promoters contain three important elements: in addition to the known (main) binding site, we identified a linker region and a secondary binding site that are crucial for functionality. Initial binding to the high-affinity, low-specificity main binding site is a prerequisite for the subsequent highly specific binding of a second RR dimer to the low-affinity secondary binding site. In addition to this hierarchical cooperative binding, discrimination requires a competition of the two RRs for their respective binding site mediated by only slight differences in binding affinities.
- Date of acceptance
- 2016
- Autoren
- Chong Fang
- Anna Nagy-Staroń
- Martin Grafe
- Ralf Heermann
- Kirsten Jung
- Susanne Gebhard
- Thorsten Mascher
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/27997719
- DOI
- 10.1111/mmi.13597
- eISSN
- 1365-2958
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Mol Microbiol
- Schlüsselwörter
- ATP-Binding Cassette Transporters
- Anti-Bacterial Agents
- Bacillus subtilis
- Bacitracin
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Nisin
- Promoter Regions, Genetic
- Protein Binding
- Regulatory Sequences, Nucleic Acid
- Up-Regulation
- Sprache
- eng
- Country
- England
- Paginierung
- 16 - 31
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2017
- Titel
- Insulation and wiring specificity of BceR-like response regulators and their target promoters in Bacillus subtilis.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 104
Data source: PubMed
- Beziehungen:
- Property of