HexA is a versatile regulator involved in the control of phenotypic heterogeneity of Photorhabdus luminescens
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Angela Langer
- Adriana Moldovan
- Christian Harmath
- Susan A Joyce
- David J Clarke
- Ralf Heermann
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000400383600097&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1371/journal.pone.0176535
- Externe Identifier
- Clarivate Analytics Document Solution ID: ET6EL
- PubMed Identifier: 28448559
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- PLOS ONE
- Artikelnummer
- ARTN e0176535
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Titel
- HexA is a versatile regulator involved in the control of phenotypic heterogeneity of <i>Photorhabdus luminescens</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 12
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Angela Langer
- Adriana Moldovan
- Christian Harmath
- Susan A Joyce
- David J Clarke
- Ralf Heermann
- DOI
- 10.1371/journal.pone.0176535
- Editoren
- Eric Cascales
- eISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- PLOS ONE
- Sprache
- en
- Online publication date
- 2017
- Paginierung
- e0176535 - e0176535
- Status
- Published online
- Herausgeber
- Public Library of Science (PLoS)
- Herausgeber URL
- http://dx.doi.org/10.1371/journal.pone.0176535
- Datum der Datenerfassung
- 2020
- Titel
- HexA is a versatile regulator involved in the control of phenotypic heterogeneity of Photorhabdus luminescens
- Ausgabe der Zeitschrift
- 12
Data source: Crossref
- Abstract
- Phenotypic heterogeneity in microbial communities enables genetically identical organisms to behave differently even under the same environmental conditions. Photorhabdus luminescens, a bioluminescent Gram-negative bacterium, contains a complex life cycle, which involves a symbiotic interaction with nematodes as well as a pathogenic association with insect larvae. P. luminescens exists in two distinct phenotypic cell types, designated as the primary (1°) and secondary (2°) cells. The 1° cells are bioluminescent, pigmented and can support nematode growth and development. Individual 1° cells undergo phenotypic switching after prolonged cultivation and convert to 2° cells, which lack the 1° specific phenotypes. The LysR-type regulator HexA has been described as major regulator of this switching process. Here we show that HexA controls phenotypic heterogeneity in a versatile way, directly and indirectly. Expression of hexA is enhanced in 2° cells, and the corresponding regulator inhibits 1° specific traits in 2° cells. HexA does not directly affect bioluminescence, a predominant 1° specific phenotype. Since the respective luxCDABE operon is repressed at the post-transcriptional level and transcriptional levels of the RNA chaperone gene hfq are also enhanced in 2° cells, small regulatory RNAs are presumably involved that are under control of HexA. Another phenotypic trait that is specific for 1° cells is quorum sensing mediated cell clumping. The corresponding pcfABCDEF operon could be identified as the first direct target of HexA, since the regulator binds to the pcfA promoter region and thereby blocks expression of the target operon. In summary, our data show that HexA fulfills the task as repressor of 1° specific features in 2° cells in a versatile way and gives first insights into the complexity of regulating phenotypic heterogeneity in Photorhabdus bacteria.
- Addresses
- Bereich Mikrobiologie, Biozentrum Martinsried, Ludwig-Maximilians-Universität München, München, Germany.
- Autoren
- Angela Langer
- Adriana Moldovan
- Christian Harmath
- Susan A Joyce
- David J Clarke
- Ralf Heermann
- DOI
- 10.1371/journal.pone.0176535
- eISSN
- 1932-6203
- Externe Identifier
- PubMed Identifier: 28448559
- PubMed Central ID: PMC5407808
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: Schwerpunkt SPP1617 (HE 5247/5-1)
- Open access
- true
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- PloS one
- Schlüsselwörter
- Photorhabdus
- Bacterial Proteins
- Host Factor 1 Protein
- DNA-Binding Proteins
- Luminescent Measurements
- Transcription, Genetic
- Phenotype
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2017
- Open access status
- Open Access
- Paginierung
- e0176535
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2017
- Titel
- HexA is a versatile regulator involved in the control of phenotypic heterogeneity of Photorhabdus luminescens.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 12
Files
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0176535&type=printable https://europepmc.org/articles/PMC5407808?pdf=render
Data source: Europe PubMed Central
- Abstract
- Phenotypic heterogeneity in microbial communities enables genetically identical organisms to behave differently even under the same environmental conditions. Photorhabdus luminescens, a bioluminescent Gram-negative bacterium, contains a complex life cycle, which involves a symbiotic interaction with nematodes as well as a pathogenic association with insect larvae. P. luminescens exists in two distinct phenotypic cell types, designated as the primary (1°) and secondary (2°) cells. The 1° cells are bioluminescent, pigmented and can support nematode growth and development. Individual 1° cells undergo phenotypic switching after prolonged cultivation and convert to 2° cells, which lack the 1° specific phenotypes. The LysR-type regulator HexA has been described as major regulator of this switching process. Here we show that HexA controls phenotypic heterogeneity in a versatile way, directly and indirectly. Expression of hexA is enhanced in 2° cells, and the corresponding regulator inhibits 1° specific traits in 2° cells. HexA does not directly affect bioluminescence, a predominant 1° specific phenotype. Since the respective luxCDABE operon is repressed at the post-transcriptional level and transcriptional levels of the RNA chaperone gene hfq are also enhanced in 2° cells, small regulatory RNAs are presumably involved that are under control of HexA. Another phenotypic trait that is specific for 1° cells is quorum sensing mediated cell clumping. The corresponding pcfABCDEF operon could be identified as the first direct target of HexA, since the regulator binds to the pcfA promoter region and thereby blocks expression of the target operon. In summary, our data show that HexA fulfills the task as repressor of 1° specific features in 2° cells in a versatile way and gives first insights into the complexity of regulating phenotypic heterogeneity in Photorhabdus bacteria.
- Date of acceptance
- 2017
- Autoren
- Angela Langer
- Adriana Moldovan
- Christian Harmath
- Susan A Joyce
- David J Clarke
- Ralf Heermann
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/28448559
- DOI
- 10.1371/journal.pone.0176535
- eISSN
- 1932-6203
- Externe Identifier
- PubMed Central ID: PMC5407808
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- PLoS One
- Schlüsselwörter
- Bacterial Proteins
- DNA-Binding Proteins
- Host Factor 1 Protein
- Luminescent Measurements
- Phenotype
- Photorhabdus
- Transcription, Genetic
- Sprache
- eng
- Country
- United States
- Paginierung
- e0176535
- PII
- PONE-D-16-45418
- Datum der Veröffentlichung
- 2017
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2017
- Titel
- HexA is a versatile regulator involved in the control of phenotypic heterogeneity of Photorhabdus luminescens.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 12
Data source: PubMed
- Beziehungen:
- Property of