Oxygen-Releasing Hyaluronic Acid-Based Dispersion with Controlled Oxygen Delivery for Enhanced Periodontal Tissue Engineering
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Lena Katharina Mueller-Heupt
- Nadine Wiesmann-Imilowski
- Sofia Schroeder
- Jonathan Gross
- Pablo Cores Ziskoven
- Philipp Bani
- Peer Wolfgang Kaemmerer
- Eik Schiegnitz
- Anja Eckelt
- John Eckelt
- Ulrike Ritz
- Till Opatz
- Bilal Al-Nawas
- Christopher V Synatschke
- James Deschner
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000955668800001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/ijms24065936
- eISSN
- 1422-0067
- Externe Identifier
- Clarivate Analytics Document Solution ID: A5QQ1
- PubMed Identifier: 36983008
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Schlüsselwörter
- angiogenesis
- biopolymer
- hyaluronic acid
- hypoxia
- tissue regeneration
- tissue engineering
- oxygen
- peroxides
- periodontitis
- Artikelnummer
- ARTN 5936
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Titel
- Oxygen-Releasing Hyaluronic Acid-Based Dispersion with Controlled Oxygen Delivery for Enhanced Periodontal Tissue Engineering
- Sub types
- Article
- Ausgabe der Zeitschrift
- 24
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p>Periodontitis is a chronic biofilm-associated inflammatory disease of the tooth-supporting tissues that causes tooth loss. It is strongly associated with anaerobic bacterial colonization and represents a substantial global health burden. Due to a local hypoxic environment, tissue regeneration is impaired. Oxygen therapy has shown promising results as a potential treatment of periodontitis, but so far, local oxygen delivery remains a key technical challenge. An oxygen (O2)-releasing hyaluronic acid (HA)-based dispersion with a controlled oxygen delivery was developed. Cell viability of primary human fibroblasts, osteoblasts, and HUVECs was demonstrated, and biocompatibility was tested using a chorioallantoic membrane assay (CAM assay). Suppression of anaerobic growth of Porphyromonas gingivalis was shown using the broth microdilution assay. In vitro assays showed that the O2-releasing HA was not cytotoxic towards human primary fibroblasts, osteoblasts, and HUVECs. In vivo, angiogenesis was enhanced in a CAM assay, although not to a statistically significant degree. Growth of P. gingivalis was inhibited by CaO2 concentrations higher than 256 mg/L. Taken together, the results of this study demonstrate the biocompatibility and selective antimicrobial activity against P. gingivalis for the developed O2-releasing HA-based dispersion and the potential of O2-releasing biomaterials for periodontal tissue regeneration.</jats:p>
- Autoren
- Lena Katharina Müller-Heupt
- Nadine Wiesmann-Imilowski
- Sofia Schröder
- Jonathan Groß
- Pablo Cores Ziskoven
- Philipp Bani
- Peer Wolfgang Kämmerer
- Eik Schiegnitz
- Anja Eckelt
- John Eckelt
- Ulrike Ritz
- Till Opatz
- Bilal Al-Nawas
- Christopher V Synatschke
- James Deschner
- DOI
- 10.3390/ijms24065936
- eISSN
- 1422-0067
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- International Journal of Molecular Sciences
- Sprache
- en
- Online publication date
- 2023
- Paginierung
- 5936 - 5936
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/ijms24065936
- Datum der Datenerfassung
- 2023
- Titel
- Oxygen-Releasing Hyaluronic Acid-Based Dispersion with Controlled Oxygen Delivery for Enhanced Periodontal Tissue Engineering
- Ausgabe der Zeitschrift
- 24
Data source: Crossref
- Abstract
- Periodontitis is a chronic biofilm-associated inflammatory disease of the tooth-supporting tissues that causes tooth loss. It is strongly associated with anaerobic bacterial colonization and represents a substantial global health burden. Due to a local hypoxic environment, tissue regeneration is impaired. Oxygen therapy has shown promising results as a potential treatment of periodontitis, but so far, local oxygen delivery remains a key technical challenge. An oxygen (O<sub>2</sub>)-releasing hyaluronic acid (HA)-based dispersion with a controlled oxygen delivery was developed. Cell viability of primary human fibroblasts, osteoblasts, and HUVECs was demonstrated, and biocompatibility was tested using a chorioallantoic membrane assay (CAM assay). Suppression of anaerobic growth of <i>Porphyromonas gingivalis</i> was shown using the broth microdilution assay. In vitro assays showed that the O<sub>2</sub>-releasing HA was not cytotoxic towards human primary fibroblasts, osteoblasts, and HUVECs. In vivo, angiogenesis was enhanced in a CAM assay, although not to a statistically significant degree. Growth of <i>P. gingivalis</i> was inhibited by CaO<sub>2</sub> concentrations higher than 256 mg/L. Taken together, the results of this study demonstrate the biocompatibility and selective antimicrobial activity against <i>P. gingivalis</i> for the developed O<sub>2</sub>-releasing HA-based dispersion and the potential of O<sub>2</sub>-releasing biomaterials for periodontal tissue regeneration.
- Addresses
- Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, Germany.
- Autoren
- Lena Katharina Müller-Heupt
- Nadine Wiesmann-Imilowski
- Sofia Schröder
- Jonathan Groß
- Pablo Cores Ziskoven
- Philipp Bani
- Peer Wolfgang Kämmerer
- Eik Schiegnitz
- Anja Eckelt
- John Eckelt
- Ulrike Ritz
- Till Opatz
- Bilal Al-Nawas
- Christopher V Synatschke
- James Deschner
- DOI
- 10.3390/ijms24065936
- eISSN
- 1422-0067
- Externe Identifier
- PubMed Identifier: 36983008
- PubMed Central ID: PMC10059003
- Funding acknowledgements
- German Federal Ministry of Education and Research: 16LW0128
- Federal Ministry of Education and Research: Go-Bio 16LW0128
- Open access
- true
- ISSN
- 1422-0067
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- International journal of molecular sciences
- Schlüsselwörter
- Humans
- Porphyromonas gingivalis
- Periodontitis
- Oxygen
- Hyaluronic Acid
- Tissue Engineering
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2023
- Open access status
- Open Access
- Paginierung
- 5936
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2023
- Titel
- Oxygen-Releasing Hyaluronic Acid-Based Dispersion with Controlled Oxygen Delivery for Enhanced Periodontal Tissue Engineering.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 24
Files
https://www.mdpi.com/1422-0067/24/6/5936/pdf?version=1679394173 https://europepmc.org/articles/PMC10059003?pdf=render
Data source: Europe PubMed Central
- Abstract
- Periodontitis is a chronic biofilm-associated inflammatory disease of the tooth-supporting tissues that causes tooth loss. It is strongly associated with anaerobic bacterial colonization and represents a substantial global health burden. Due to a local hypoxic environment, tissue regeneration is impaired. Oxygen therapy has shown promising results as a potential treatment of periodontitis, but so far, local oxygen delivery remains a key technical challenge. An oxygen (O2)-releasing hyaluronic acid (HA)-based dispersion with a controlled oxygen delivery was developed. Cell viability of primary human fibroblasts, osteoblasts, and HUVECs was demonstrated, and biocompatibility was tested using a chorioallantoic membrane assay (CAM assay). Suppression of anaerobic growth of Porphyromonas gingivalis was shown using the broth microdilution assay. In vitro assays showed that the O2-releasing HA was not cytotoxic towards human primary fibroblasts, osteoblasts, and HUVECs. In vivo, angiogenesis was enhanced in a CAM assay, although not to a statistically significant degree. Growth of P. gingivalis was inhibited by CaO2 concentrations higher than 256 mg/L. Taken together, the results of this study demonstrate the biocompatibility and selective antimicrobial activity against P. gingivalis for the developed O2-releasing HA-based dispersion and the potential of O2-releasing biomaterials for periodontal tissue regeneration.
- Date of acceptance
- 2023
- Autoren
- Lena Katharina Müller-Heupt
- Nadine Wiesmann-Imilowski
- Sofia Schröder
- Jonathan Groß
- Pablo Cores Ziskoven
- Philipp Bani
- Peer Wolfgang Kämmerer
- Eik Schiegnitz
- Anja Eckelt
- John Eckelt
- Ulrike Ritz
- Till Opatz
- Bilal Al-Nawas
- Christopher V Synatschke
- James Deschner
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/36983008
- DOI
- 10.3390/ijms24065936
- eISSN
- 1422-0067
- Externe Identifier
- PubMed Central ID: PMC10059003
- Funding acknowledgements
- Federal Ministry of Education and Research: Go-Bio 16LW0128
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- Int J Mol Sci
- Schlüsselwörter
- angiogenesis
- biopolymer
- hyaluronic acid
- hypoxia
- oxygen
- periodontitis
- peroxides
- tissue engineering
- tissue regeneration
- Humans
- Hyaluronic Acid
- Tissue Engineering
- Oxygen
- Porphyromonas gingivalis
- Periodontitis
- Sprache
- eng
- Country
- Switzerland
- PII
- ijms24065936
- Datum der Veröffentlichung
- 2023
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2023
- Titel
- Oxygen-Releasing Hyaluronic Acid-Based Dispersion with Controlled Oxygen Delivery for Enhanced Periodontal Tissue Engineering.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 24
Data source: PubMed
- Beziehungen:
- Property of