Resilience and stability of the CF- intestinal and respiratory microbiome during nutritional and exercise intervention

Publication type:
Journal article
Metadata:
Autoren
  • Rebecca L Knoll
  • Victor Hugo Jarquin-Diaz
  • Jonas Klopp
  • Alissa Kemper
  • Katja Hilbert
  • Barlo Hillen
  • Daniel Pfirrmann
  • Perikles Simon
  • Viola Baehner
  • Oliver Nitsche
  • Stephan Gehring
  • Lajos Marko
  • Sofia K Forslund
  • Krystyna Poplawska
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000934534400001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1186/s12866-023-02788-y
Externe Identifier
  • Clarivate Analytics Document Solution ID: 9B1WJ
  • PubMed Identifier: 36803565
ISSN
1471-2180
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC MICROBIOLOGY
Schlüsselwörter
  • Cystic fibrosis
  • Microbiome
  • Gut-lung axis
  • Physical exercise
  • Nutrition
  • Antibiotics
  • Intervention
Artikelnummer
ARTN 44
Datum der Veröffentlichung
2023
Status
Published
Titel
Resilience and stability of the CF- intestinal and respiratory microbiome during nutritional and exercise intervention
Sub types
  • Article
Ausgabe der Zeitschrift
23

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Impaired respiratory and intestinal microbiome composition is linked to cystic fibrosis lung disease severity. In people with cystic fibrosis (pwCF), regular exercise is recommended to delay disease progression and preserve a stable lung function. An optimal nutritional status is vital for best clinical outcomes. Our study investigated whether regular and monitored exercise and nutritional support promotes CF microbiome health.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>A personalized nutrition and exercise program promoted nutritional intake and physical fitness in 18 pwCF for 12 months. Throughout the study, patients performed strength and endurance training monitored by a sports scientist via an internet platform. After three months, food supplementation with <jats:italic>Lactobacillus rhamnosus</jats:italic> LGG was introduced. Nutritional status and physical fitness were assessed before the study started, after three and nine months. Sputum and stool were collected, and microbial composition was analyzed by 16S rRNA gene sequencing.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Sputum and stool microbiome composition remained stable and highly specific to each patient during the study period. Disease-associated pathogens dominated sputum composition. Lung disease severity and recent antibiotic treatment had the highest impact on taxonomic composition in stool and sputum microbiome. Strikingly, the long-term antibiotic treatment burden had only a minor influence.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>Despite the exercise and nutritional intervention, respiratory and intestinal microbiomes proved to be resilient. Dominant pathogens drove the composition and functionality of the microbiome. Further studies are required to understand which therapy could destabilize the dominant disease-associated microbial composition of pwCF.</jats:p> </jats:sec>
Autoren
  • Rebecca L Knoll
  • Víctor Hugo Jarquín-Díaz
  • Jonas Klopp
  • Alissa Kemper
  • Katja Hilbert
  • Barlo Hillen
  • Daniel Pfirrmann
  • Perikles Simon
  • Viola Bähner
  • Oliver Nitsche
  • Stephan Gehring
  • Lajos Markó
  • Sofia K Forslund
  • Krystyna Poplawska
DOI
10.1186/s12866-023-02788-y
eISSN
1471-2180
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC Microbiology
Sprache
en
Artikelnummer
44
Online publication date
2023
Status
Published online
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1186/s12866-023-02788-y
Datum der Datenerfassung
2023
Titel
Resilience and stability of the CF- intestinal and respiratory microbiome during nutritional and exercise intervention
Ausgabe der Zeitschrift
23

Data source: Crossref

Abstract
<h4>Background</h4>Impaired respiratory and intestinal microbiome composition is linked to cystic fibrosis lung disease severity. In people with cystic fibrosis (pwCF), regular exercise is recommended to delay disease progression and preserve a stable lung function. An optimal nutritional status is vital for best clinical outcomes. Our study investigated whether regular and monitored exercise and nutritional support promotes CF microbiome health.<h4>Methods</h4>A personalized nutrition and exercise program promoted nutritional intake and physical fitness in 18 pwCF for 12 months. Throughout the study, patients performed strength and endurance training monitored by a sports scientist via an internet platform. After three months, food supplementation with Lactobacillus rhamnosus LGG was introduced. Nutritional status and physical fitness were assessed before the study started, after three and nine months. Sputum and stool were collected, and microbial composition was analyzed by 16S rRNA gene sequencing.<h4>Results</h4>Sputum and stool microbiome composition remained stable and highly specific to each patient during the study period. Disease-associated pathogens dominated sputum composition. Lung disease severity and recent antibiotic treatment had the highest impact on taxonomic composition in stool and sputum microbiome. Strikingly, the long-term antibiotic treatment burden had only a minor influence.<h4>Conclusion</h4>Despite the exercise and nutritional intervention, respiratory and intestinal microbiomes proved to be resilient. Dominant pathogens drove the composition and functionality of the microbiome. Further studies are required to understand which therapy could destabilize the dominant disease-associated microbial composition of pwCF.
Addresses
  • Pediatric Pulmonology, Allergology and Cystic Fibrosis, Children's Hospital, University Medical Center of the Johannes Gutenberg-University Mainz, Germany; Langenbeckstraße 1, 55131, Mainz, Germany.
Autoren
  • Rebecca L Knoll
  • Víctor Hugo Jarquín-Díaz
  • Jonas Klopp
  • Alissa Kemper
  • Katja Hilbert
  • Barlo Hillen
  • Daniel Pfirrmann
  • Perikles Simon
  • Viola Bähner
  • Oliver Nitsche
  • Stephan Gehring
  • Lajos Markó
  • Sofia K Forslund
  • Krystyna Poplawska
DOI
10.1186/s12866-023-02788-y
eISSN
1471-2180
Externe Identifier
  • PubMed Identifier: 36803565
  • PubMed Central ID: PMC9942320
Funding acknowledgements
  • Universitätsmedizin der Johannes Gutenberg-Universität Mainz:
  • Vertex Pharmaceuticals: Circle of Care
  • Bundesministerium für Bildung und Forschung: F01KI1909A
Open access
true
ISSN
1471-2180
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC microbiology
Schlüsselwörter
  • Sputum
  • Humans
  • Cystic Fibrosis
  • RNA, Ribosomal, 16S
  • Anti-Bacterial Agents
  • Exercise Therapy
  • Microbiota
Sprache
eng
Medium
Electronic
Online publication date
2023
Open access status
Open Access
Paginierung
44
Datum der Veröffentlichung
2023
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2023
Titel
Resilience and stability of the CF- intestinal and respiratory microbiome during nutritional and exercise intervention.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
23

Files

https://bmcmicrobiol.biomedcentral.com/counter/pdf/10.1186/s12866-023-02788-y https://europepmc.org/articles/PMC9942320?pdf=render

Data source: Europe PubMed Central

Abstract
BACKGROUND: Impaired respiratory and intestinal microbiome composition is linked to cystic fibrosis lung disease severity. In people with cystic fibrosis (pwCF), regular exercise is recommended to delay disease progression and preserve a stable lung function. An optimal nutritional status is vital for best clinical outcomes. Our study investigated whether regular and monitored exercise and nutritional support promotes CF microbiome health. METHODS: A personalized nutrition and exercise program promoted nutritional intake and physical fitness in 18 pwCF for 12 months. Throughout the study, patients performed strength and endurance training monitored by a sports scientist via an internet platform. After three months, food supplementation with Lactobacillus rhamnosus LGG was introduced. Nutritional status and physical fitness were assessed before the study started, after three and nine months. Sputum and stool were collected, and microbial composition was analyzed by 16S rRNA gene sequencing. RESULTS: Sputum and stool microbiome composition remained stable and highly specific to each patient during the study period. Disease-associated pathogens dominated sputum composition. Lung disease severity and recent antibiotic treatment had the highest impact on taxonomic composition in stool and sputum microbiome. Strikingly, the long-term antibiotic treatment burden had only a minor influence. CONCLUSION: Despite the exercise and nutritional intervention, respiratory and intestinal microbiomes proved to be resilient. Dominant pathogens drove the composition and functionality of the microbiome. Further studies are required to understand which therapy could destabilize the dominant disease-associated microbial composition of pwCF.
Date of acceptance
2022
Autoren
  • Rebecca L Knoll
  • Víctor Hugo Jarquín-Díaz
  • Jonas Klopp
  • Alissa Kemper
  • Katja Hilbert
  • Barlo Hillen
  • Daniel Pfirrmann
  • Perikles Simon
  • Viola Bähner
  • Oliver Nitsche
  • Stephan Gehring
  • Lajos Markó
  • Sofia K Forslund
  • Krystyna Poplawska
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/36803565
DOI
10.1186/s12866-023-02788-y
eISSN
1471-2180
Externe Identifier
  • PubMed Central ID: PMC9942320
Funding acknowledgements
  • Bundesministerium für Bildung und Forschung: F01KI1909A
  • Vertex Pharmaceuticals: Circle of Care
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC Microbiol
Schlüsselwörter
  • Antibiotics
  • Cystic fibrosis
  • Gut-lung axis
  • Intervention
  • Microbiome
  • Nutrition
  • Physical exercise
  • Humans
  • Cystic Fibrosis
  • RNA, Ribosomal, 16S
  • Microbiota
  • Sputum
  • Anti-Bacterial Agents
  • Exercise Therapy
Sprache
eng
Country
England
Paginierung
44
PII
10.1186/s12866-023-02788-y
Datum der Veröffentlichung
2023
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2023
Titel
Resilience and stability of the CF- intestinal and respiratory microbiome during nutritional and exercise intervention.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
23

Data source: PubMed

Author's licence
CC-BY
Autoren
  • Rebecca L Knoll
  • Víctor Hugo Jarquín‑Díaz
  • Jonas Klopp
  • Alissa Kemper
  • Katja Hilbert
  • Barlo Hillen
  • Daniel Pfirrmann
  • Perikles Simon
  • Viola Bähner
  • Oliver Nitsche
  • Stephan Gehring
  • Lajos Markó
  • Sofia K Forslund
  • Krystyna Poplawska
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • DFG-491381577-G
Resource version
Published version
DOI
10.1186/s12866-023-02788-y
Funding acknowledgements
  • Deutsche Forschungsgemeinschaft (DFG)|491381577|Open-Access-Publikationskosten 2022–2024 Universität Mainz - Universitätsmedizin
File(s) embargoed
false
Open access
true
ISSN
1471-2180
Zeitschrift
BMC microbiology
Schlüsselwörter
  • 610 Medizin
  • 610 Medical sciences
Sprache
eng
Open access status
Open Access
Paginierung
44
Datum der Veröffentlichung
2023
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/9456
Herausgeber
BioMed Central
Herausgeber URL
https://doi.org/10.1186/s12866-023-02788-y
Datum der Datenerfassung
2023
Datum, an dem der Datensatz öffentlich gemacht wurde
2023
Zugang
Public
Titel
Resilience and stability of the CF- intestinal and respiratory microbiome during nutritional and exercise intervention
Ausgabe der Zeitschrift
23

Files

resilience_and_stability_of_t-20230818120442616.pdf

Data source: OPENSCIENCE.UB

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