A New Bacterial Adenosine-Derived Nucleoside as an Example of RNA Modification Damage

Publication type:
Journal article
Metadata:
Autoren
  • Larissa Bessler
  • Lea-Marie Vogt
  • Marc Lander
  • Christina Dal Magro
  • Patrick Keller
  • Jonas Kuehlborn
  • Christopher J Kampf
  • Till Opatz
  • Mark Helm
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000928874000001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1002/anie.202217128
eISSN
1521-3773
Externe Identifier
  • Clarivate Analytics Document Solution ID: P3BN7
  • PubMed Identifier: 36629490
ISSN
1433-7851
Ausgabe der Veröffentlichung
11
Zeitschrift
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Schlüsselwörter
  • Epitranscriptome
  • Isotopic Labeling
  • Mass Spectrometry
  • Nucleoside Analysis
  • RNA Modification Damage
Datum der Veröffentlichung
2023
Status
Published
Titel
A New Bacterial Adenosine-Derived Nucleoside as an Example of RNA Modification Damage
Sub types
  • Article
Ausgabe der Zeitschrift
62

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title><jats:p>The fields of RNA modification and RNA damage both exhibit a plethora of non‐canonical nucleoside structures. While RNA modifications have evolved to improve RNA function, the term RNA damage implies detrimental effects. Based on stable isotope labelling and mass spectrometry, we report the identification and characterisation of 2‐methylthio‐1,<jats:italic>N</jats:italic>6‐ethenoadenosine (ms<jats:sup>2</jats:sup>ϵA), which is related to 1,<jats:italic>N</jats:italic>6‐ethenoadenine, a lesion resulting from exposure of nucleic acids to alkylating chemicals in vivo. In contrast, a sophisticated isoprene labelling scheme revealed that ms<jats:sup>2</jats:sup>ϵA biogenesis involves cleavage of a prenyl moiety in the known transfer RNA (tRNA) modification 2‐methylthio‐<jats:italic>N</jats:italic>6‐isopentenyladenosine (ms<jats:sup>2</jats:sup>i<jats:sup>6</jats:sup>A). The relative abundance of ms<jats:sup>2</jats:sup>ϵA in tRNAs from translating ribosomes suggests reduced function in comparison to its parent RNA modification, establishing the nature of the new structure in a newly perceived overlap of the two previously separate fields, namely an RNA modification damage.</jats:p>
Autoren
  • Larissa Bessler
  • Lea‐Marie Vogt
  • Marc Lander
  • Christina Dal Magro
  • Patrick Keller
  • Jonas Kühlborn
  • Christopher J Kampf
  • Till Opatz
  • Mark Helm
DOI
10.1002/anie.202217128
eISSN
1521-3773
ISSN
1433-7851
Ausgabe der Veröffentlichung
11
Zeitschrift
Angewandte Chemie International Edition
Sprache
en
Online publication date
2023
Datum der Veröffentlichung
2023
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1002/anie.202217128
Datum der Datenerfassung
2023
Titel
A New Bacterial Adenosine‐Derived Nucleoside as an Example of RNA Modification Damage
Ausgabe der Zeitschrift
62

Data source: Crossref

Abstract
The fields of RNA modification and RNA damage both exhibit a plethora of non-canonical nucleoside structures. While RNA modifications have evolved to improve RNA function, the term RNA damage implies detrimental effects. Based on stable isotope labelling and mass spectrometry, we report the identification and characterisation of 2-methylthio-1,N6-ethenoadenosine (ms<sup>2</sup> ϵA), which is related to 1,N6-ethenoadenine, a lesion resulting from exposure of nucleic acids to alkylating chemicals in vivo. In contrast, a sophisticated isoprene labelling scheme revealed that ms<sup>2</sup> ϵA biogenesis involves cleavage of a prenyl moiety in the known transfer RNA (tRNA) modification 2-methylthio-N6-isopentenyladenosine (ms<sup>2</sup> i<sup>6</sup> A). The relative abundance of ms<sup>2</sup> ϵA in tRNAs from translating ribosomes suggests reduced function in comparison to its parent RNA modification, establishing the nature of the new structure in a newly perceived overlap of the two previously separate fields, namely an RNA modification damage.
Addresses
  • Institute of Pharmaceutical and Biomedical Sciences (IPBS), Johannes Gutenberg University Mainz, Staudingerweg 5, 55128, Mainz, Germany.
Autoren
  • Larissa Bessler
  • Lea-Marie Vogt
  • Marc Lander
  • Christina Dal Magro
  • Patrick Keller
  • Jonas Kühlborn
  • Christopher J Kampf
  • Till Opatz
  • Mark Helm
DOI
10.1002/anie.202217128
eISSN
1521-3773
Externe Identifier
  • PubMed Identifier: 36629490
Funding acknowledgements
  • Deutsche Forschungsgemeinschaft: HE 3397/14-2 in SPP1784
  • Deutsche Forschungsgemeinschaft: TP C03 in TRR 319 Project-ID 439669440
Open access
false
ISSN
1433-7851
Ausgabe der Veröffentlichung
11
Zeitschrift
Angewandte Chemie (International ed. in English)
Schlüsselwörter
  • Nucleosides
  • RNA
  • RNA, Bacterial
  • RNA, Transfer
  • Adenosine
Sprache
eng
Medium
Print-Electronic
Online publication date
2023
Paginierung
e202217128
Datum der Veröffentlichung
2023
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2023
Titel
A New Bacterial Adenosine-Derived Nucleoside as an Example of RNA Modification Damage.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
62

Data source: Europe PubMed Central

Abstract
The fields of RNA modification and RNA damage both exhibit a plethora of non-canonical nucleoside structures. While RNA modifications have evolved to improve RNA function, the term RNA damage implies detrimental effects. Based on stable isotope labelling and mass spectrometry, we report the identification and characterisation of 2-methylthio-1,N6-ethenoadenosine (ms2 ϵA), which is related to 1,N6-ethenoadenine, a lesion resulting from exposure of nucleic acids to alkylating chemicals in vivo. In contrast, a sophisticated isoprene labelling scheme revealed that ms2 ϵA biogenesis involves cleavage of a prenyl moiety in the known transfer RNA (tRNA) modification 2-methylthio-N6-isopentenyladenosine (ms2 i6 A). The relative abundance of ms2 ϵA in tRNAs from translating ribosomes suggests reduced function in comparison to its parent RNA modification, establishing the nature of the new structure in a newly perceived overlap of the two previously separate fields, namely an RNA modification damage.
Autoren
  • Larissa Bessler
  • Lea-Marie Vogt
  • Marc Lander
  • Christina Dal Magro
  • Patrick Keller
  • Jonas Kühlborn
  • Christopher J Kampf
  • Till Opatz
  • Mark Helm
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/36629490
DOI
10.1002/anie.202217128
eISSN
1521-3773
Ausgabe der Veröffentlichung
11
Zeitschrift
Angew Chem Int Ed Engl
Schlüsselwörter
  • Epitranscriptome
  • Isotopic Labeling
  • Mass Spectrometry
  • Nucleoside Analysis
  • RNA Modification Damage
  • Nucleosides
  • Adenosine
  • RNA, Transfer
  • RNA
  • RNA, Bacterial
Sprache
eng
Country
Germany
Paginierung
e202217128
Datum der Veröffentlichung
2023
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2023
Titel
A New Bacterial Adenosine-Derived Nucleoside as an Example of RNA Modification Damage.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
62

Data source: PubMed

Author's licence
CC-BY
Autoren
  • Larissa Bessler
  • Lea-Marie Vogt
  • Marc Lander
  • Christina Dal Magro
  • Patrick Keller
  • Jonas Kühlborn
  • Christopher J Kampf
  • Till Opatz
  • Mark Helm
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • DFG-491381577-H
Resource version
Published version
DOI
10.1002/anie.202217128
Funding acknowledgements
  • Deutsche Forschungsgemeinschaft (DFG)|491381577|Open-Access-Publikationskosten 2022–2024 Universität Mainz - Universitätsmedizin
File(s) embargoed
false
Open access
true
ISSN
1521-3773
Ausgabe der Veröffentlichung
11
Zeitschrift
Angewandte Chemie : International edition
Schlüsselwörter
  • 540 Chemie
  • 540 Chemistry and allied sciences
Sprache
eng
Open access status
Open Access
Paginierung
e202217128
Datum der Veröffentlichung
2023
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/9069
Herausgeber
Wiley-VCH
Datum der Datenerfassung
2023
Datum, an dem der Datensatz öffentlich gemacht wurde
2023
Zugang
Public
Titel
A new bacterial adenosine-derived nucleoside as an example of RNA modification damage
Ausgabe der Zeitschrift
62

Files

a_new_bacterial_adenosinederi-20230425113414785.pdf

Data source: OPENSCIENCE.UB

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