High-throughput synthesis of CeO2 nanoparticles for transparent nanocomposites repelling Pseudomonas aeruginosa biofilms
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Massih Sarif
- Olga Jegel
- Athanasios Gazanis
- Jens Hartmann
- Sergi Plana-Ruiz
- Jan Hilgert
- Hajo Frerichs
- Melanie Viel
- Martin Panthoefer
- Ute Kolb
- Muhammad Nawaz Tahir
- Jorg Schemberg
- Michael Kappl
- Ralf Heermann
- Wolfgang Tremel
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000838209800134&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1038/s41598-022-07833-w
- Externe Identifier
- Clarivate Analytics Document Solution ID: 3Q4NK
- PubMed Identifier: 35273241
- ISSN
- 2045-2322
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- SCIENTIFIC REPORTS
- Artikelnummer
- ARTN 3935
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Titel
- High-throughput synthesis of CeO<sub>2</sub> nanoparticles for transparent nanocomposites repelling <i>Pseudomonas aeruginosa</i> biofilms
- Sub types
- Article
- Ausgabe der Zeitschrift
- 12
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Preventing bacteria from adhering to material surfaces is an important technical problem and a major cause of infection. One of nature’s defense strategies against bacterial colonization is based on the biohalogenation of signal substances that interfere with bacterial communication. Biohalogenation is catalyzed by haloperoxidases, a class of metal-dependent enzymes whose activity can be mimicked by ceria nanoparticles. Transparent CeO<jats:sub>2</jats:sub>/polycarbonate surfaces that prevent adhesion, proliferation, and spread of <jats:italic>Pseudomonas aeruginosa</jats:italic> PA14 were manufactured. Large amounts of monodisperse CeO<jats:sub>2</jats:sub> nanoparticles were synthesized in segmented flow using a high-throughput microfluidic benchtop system using water/benzyl alcohol mixtures and oleylamine as capping agent. This reduced the reaction time for nanoceria by more than one order of magnitude compared to conventional batch methods. Ceria nanoparticles prepared by segmented flow showed high catalytic activity in halogenation reactions, which makes them highly efficient functional mimics of haloperoxidase enzymes. Haloperoxidases are used in nature by macroalgae to prevent formation of biofilms via halogenation of signaling compounds that interfere with bacterial cell–cell communication (“quorum sensing”). CeO<jats:sub>2</jats:sub>/polycarbonate nanocomposites were prepared by dip-coating plasma-treated polycarbonate panels in CeO<jats:sub>2</jats:sub> dispersions. These showed a reduction in bacterial biofilm formation of up to 85% using <jats:italic>P. aeruginosa</jats:italic> PA14 as model organism. Besides biofilm formation, also the production of the virulence factor pyocyanin in is under control of the entire quorum sensing systems <jats:italic>P. aeruginosa</jats:italic>. CeO<jats:sub>2</jats:sub>/PC showed a decrease of up to 55% in pyocyanin production, whereas no effect on bacterial growth in liquid culture was observed. This indicates that CeO<jats:sub>2</jats:sub> nanoparticles affect quorum sensing and inhibit biofilm formation in a non-biocidal manner.</jats:p>
- Autoren
- Massih Sarif
- Olga Jegel
- Athanasios Gazanis
- Jens Hartmann
- Sergi Plana-Ruiz
- Jan Hilgert
- Hajo Frerichs
- Melanie Viel
- Martin Panthöfer
- Ute Kolb
- Muhammad Nawaz Tahir
- Jörg Schemberg
- Michael Kappl
- Ralf Heermann
- Wolfgang Tremel
- DOI
- 10.1038/s41598-022-07833-w
- eISSN
- 2045-2322
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Scientific Reports
- Sprache
- en
- Artikelnummer
- 3935
- Online publication date
- 2022
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1038/s41598-022-07833-w
- Datum der Datenerfassung
- 2022
- Titel
- High-throughput synthesis of CeO2 nanoparticles for transparent nanocomposites repelling Pseudomonas aeruginosa biofilms
- Ausgabe der Zeitschrift
- 12
Data source: Crossref
- Abstract
- Preventing bacteria from adhering to material surfaces is an important technical problem and a major cause of infection. One of nature's defense strategies against bacterial colonization is based on the biohalogenation of signal substances that interfere with bacterial communication. Biohalogenation is catalyzed by haloperoxidases, a class of metal-dependent enzymes whose activity can be mimicked by ceria nanoparticles. Transparent CeO<sub>2</sub>/polycarbonate surfaces that prevent adhesion, proliferation, and spread of Pseudomonas aeruginosa PA14 were manufactured. Large amounts of monodisperse CeO<sub>2</sub> nanoparticles were synthesized in segmented flow using a high-throughput microfluidic benchtop system using water/benzyl alcohol mixtures and oleylamine as capping agent. This reduced the reaction time for nanoceria by more than one order of magnitude compared to conventional batch methods. Ceria nanoparticles prepared by segmented flow showed high catalytic activity in halogenation reactions, which makes them highly efficient functional mimics of haloperoxidase enzymes. Haloperoxidases are used in nature by macroalgae to prevent formation of biofilms via halogenation of signaling compounds that interfere with bacterial cell-cell communication ("quorum sensing"). CeO<sub>2</sub>/polycarbonate nanocomposites were prepared by dip-coating plasma-treated polycarbonate panels in CeO<sub>2</sub> dispersions. These showed a reduction in bacterial biofilm formation of up to 85% using P. aeruginosa PA14 as model organism. Besides biofilm formation, also the production of the virulence factor pyocyanin in is under control of the entire quorum sensing systems P. aeruginosa. CeO<sub>2</sub>/PC showed a decrease of up to 55% in pyocyanin production, whereas no effect on bacterial growth in liquid culture was observed. This indicates that CeO<sub>2</sub> nanoparticles affect quorum sensing and inhibit biofilm formation in a non-biocidal manner.
- Addresses
- Department Chemie, Johannes Gutenberg-Universität Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.
- Autoren
- Massih Sarif
- Olga Jegel
- Athanasios Gazanis
- Jens Hartmann
- Sergi Plana-Ruiz
- Jan Hilgert
- Hajo Frerichs
- Melanie Viel
- Martin Panthöfer
- Ute Kolb
- Muhammad Nawaz Tahir
- Jörg Schemberg
- Michael Kappl
- Ralf Heermann
- Wolfgang Tremel
- DOI
- 10.1038/s41598-022-07833-w
- eISSN
- 2045-2322
- Externe Identifier
- PubMed Identifier: 35273241
- PubMed Central ID: PMC8913809
- Funding acknowledgements
- Johannes Gutenberg-Universität Mainz:
- Bundesministerium für Bildung und Forschung: 19585N
- Bundesministerium für Bildung und Forschung: 031B0583A
- Deutsche Forschungsgemeinschaft: TR 210/38-1
- Open access
- true
- ISSN
- 2045-2322
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Scientific reports
- Schlüsselwörter
- Bacteria
- Biofilms
- Pseudomonas aeruginosa
- Pyocyanine
- Virulence Factors
- Anti-Bacterial Agents
- Nanoparticles
- Quorum Sensing
- Nanocomposites
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2022
- Open access status
- Open Access
- Paginierung
- 3935
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2022
- Titel
- High-throughput synthesis of CeO<sub>2</sub> nanoparticles for transparent nanocomposites repelling Pseudomonas aeruginosa biofilms.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 12
Files
https://www.nature.com/articles/s41598-022-07833-w.pdf https://europepmc.org/articles/PMC8913809?pdf=render
Data source: Europe PubMed Central
- Abstract
- Preventing bacteria from adhering to material surfaces is an important technical problem and a major cause of infection. One of nature's defense strategies against bacterial colonization is based on the biohalogenation of signal substances that interfere with bacterial communication. Biohalogenation is catalyzed by haloperoxidases, a class of metal-dependent enzymes whose activity can be mimicked by ceria nanoparticles. Transparent CeO2/polycarbonate surfaces that prevent adhesion, proliferation, and spread of Pseudomonas aeruginosa PA14 were manufactured. Large amounts of monodisperse CeO2 nanoparticles were synthesized in segmented flow using a high-throughput microfluidic benchtop system using water/benzyl alcohol mixtures and oleylamine as capping agent. This reduced the reaction time for nanoceria by more than one order of magnitude compared to conventional batch methods. Ceria nanoparticles prepared by segmented flow showed high catalytic activity in halogenation reactions, which makes them highly efficient functional mimics of haloperoxidase enzymes. Haloperoxidases are used in nature by macroalgae to prevent formation of biofilms via halogenation of signaling compounds that interfere with bacterial cell-cell communication ("quorum sensing"). CeO2/polycarbonate nanocomposites were prepared by dip-coating plasma-treated polycarbonate panels in CeO2 dispersions. These showed a reduction in bacterial biofilm formation of up to 85% using P. aeruginosa PA14 as model organism. Besides biofilm formation, also the production of the virulence factor pyocyanin in is under control of the entire quorum sensing systems P. aeruginosa. CeO2/PC showed a decrease of up to 55% in pyocyanin production, whereas no effect on bacterial growth in liquid culture was observed. This indicates that CeO2 nanoparticles affect quorum sensing and inhibit biofilm formation in a non-biocidal manner.
- Date of acceptance
- 2022
- Autoren
- Massih Sarif
- Olga Jegel
- Athanasios Gazanis
- Jens Hartmann
- Sergi Plana-Ruiz
- Jan Hilgert
- Hajo Frerichs
- Melanie Viel
- Martin Panthöfer
- Ute Kolb
- Muhammad Nawaz Tahir
- Jörg Schemberg
- Michael Kappl
- Ralf Heermann
- Wolfgang Tremel
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/35273241
- DOI
- 10.1038/s41598-022-07833-w
- eISSN
- 2045-2322
- Externe Identifier
- PubMed Central ID: PMC8913809
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Sci Rep
- Schlüsselwörter
- Anti-Bacterial Agents
- Bacteria
- Biofilms
- Nanocomposites
- Nanoparticles
- Pseudomonas aeruginosa
- Pyocyanine
- Quorum Sensing
- Virulence Factors
- Sprache
- eng
- Country
- England
- Paginierung
- 3935
- PII
- 10.1038/s41598-022-07833-w
- Datum der Veröffentlichung
- 2022
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Titel
- High-throughput synthesis of CeO2 nanoparticles for transparent nanocomposites repelling Pseudomonas aeruginosa biofilms.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 12
Data source: PubMed
- Author's licence
- CC-BY
- Autoren
- Massih Sarif
- Olga Jegel
- Athanasios Gazanis
- Jens Hartmann
- Sergi Plana-Ruiz
- Jan Hilgert
- Hajo Frerichs
- Melanie Viel
- Martin Panthöfer
- Ute Kolb
- Muhammad Nawaz Tahir
- Jörg Schemberg
- Michael Kappl
- Ralf Heermann
- Wolfgang Tremel
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- DFG-491381577-G
- Resource version
- Published version
- DOI
- 10.1038/s41598-022-07833-w
- Funding acknowledgements
- Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 491381577
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 2045-2322
- Zeitschrift
- Scientific reports
- Schlüsselwörter
- 570 Biowissenschaften
- 570 Life sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- 3935
- Datum der Veröffentlichung
- 2022
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/8183
- Herausgeber
- Springer Nature
- Datum der Datenerfassung
- 2022
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Zugang
- Public
- Titel
- High-throughput synthesis of CeO2 nanoparticles for transparent nanocomposites repelling Pseudomonas aeruginosa biofilms
- Ausgabe der Zeitschrift
- 12
Files
highthroughput_synthesis_of_c-20221025103939693.pdf
Data source: OPENSCIENCE.UB
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