Thrombospondin 1 as possible key factor in the hemocompatibility of endocoronary prostheses

Publication type:
Journal article
Metadata:
Autoren
  • J Hoffmann
  • P Simon
  • AK Zimmermann
  • M Lemancyk
  • T Walter
  • M Beyer
  • HF Hoffmeister
  • G Ziemer
  • HP Wendel
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000228699800017&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.biomaterials.2005.01.016
Externe Identifier
  • Clarivate Analytics Document Solution ID: 920OK
  • PubMed Identifier: 15792551
ISSN
0142-9612
Ausgabe der Veröffentlichung
25
Zeitschrift
BIOMATERIALS
Schlüsselwörter
  • stent
  • biocompatibility
  • thrombospondin 1
  • DNA-microarray
Paginierung
5240 - 5250
Datum der Veröffentlichung
2005
Status
Published
Titel
Thrombospondin 1 as possible key factor in the hemocompatibility of endocoronary prostheses
Sub types
  • Article
Ausgabe der Zeitschrift
26

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Jan Hoffmann
  • Perikles Simon
  • Anja K Zimmermann
  • Michael Lemancyk
  • Thomas Walter
  • Martin Beyer
  • Hans-Martin Hoffmeister
  • Gerhard Ziemer
  • Hans P Wendel
DOI
10.1016/j.biomaterials.2005.01.016
ISSN
0142-9612
Ausgabe der Veröffentlichung
25
Zeitschrift
Biomaterials
Sprache
en
Paginierung
5240 - 5250
Datum der Veröffentlichung
2005
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.biomaterials.2005.01.016
Datum der Datenerfassung
2019
Titel
Thrombospondin 1 as possible key factor in the hemocompatibility of endocoronary prostheses
Ausgabe der Zeitschrift
26

Data source: Crossref

Abstract
Intracoronary stenting has markedly improved the patency of native coronary arteries after percutaneous transluminal coronary angioplasty (PTCA). Advances in stent technology and design, including drug releasing stents, have contributed to reduce the long-term restenosis rate. However, stenosis caused by neointimal hyperplasia, vascular remodeling and thrombosis is still a major problem after endocoronary stent procedures. This study focuses on differential gene expression of circulating peripheral blood cells after 90 min exposure to stents to search for initially activated cellular pathways, which may foster restenosis. Fresh human whole blood (1 IU heparin/ml), taken from non-medicated healthy volunteers, was incubated under flow conditions in an in vitro closed-loop stent-testing model (modified Chandler-Loop). Differential gene expression compared to resting conditions and to the experimental controls was investigated by a DNA-microarray technique encoding for over 17,000 genes simultaneously. As expected, a large variety of genes showed differential gene expression. Interestingly, Thrombospondin 1 (TSP-1), which plays a key role in initial immune defense, was found to be the most markedly up-regulated gene. We propose TSP-1 expression as an early indicator for the activation of immune responses following intracoronary stenting. After clarifying the participation of TSP-1 in vivo, future studies will therefore focus on TSP-1 as a potential prognostic factor, which may also help to develop and control new surface materials with an improved biocompatibility.
Addresses
  • Department of Thoracic, Cardiac and Vascular Surgery, University Hospital Tuebingen, 72076 Tuebingen, Germany. jan.hoffman@uni-tuebingen.de
Autoren
  • Jan Hoffmann
  • Perikles Simon
  • Anja K Zimmermann
  • Michael Lemancyk
  • Thomas Walter
  • Martin Beyer
  • Hans-Martin Hoffmeister
  • Gerhard Ziemer
  • Hans P Wendel
DOI
10.1016/j.biomaterials.2005.01.016
eISSN
1878-5905
Externe Identifier
  • PubMed Identifier: 15792551
Open access
false
ISSN
0142-9612
Ausgabe der Veröffentlichung
25
Zeitschrift
Biomaterials
Schlüsselwörter
  • Humans
  • Coronary Restenosis
  • Thrombospondin 1
  • Biocompatible Materials
  • Oligonucleotide Array Sequence Analysis
  • Gene Expression Profiling
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stents
  • Gene Expression
  • Down-Regulation
  • Up-Regulation
  • Models, Biological
  • Adult
  • Middle Aged
  • In Vitro Techniques
Sprache
eng
Medium
Print
Paginierung
5240 - 5250
Datum der Veröffentlichung
2005
Status
Published
Datum der Datenerfassung
2005
Titel
Thrombospondin 1 as possible key factor in the hemocompatibility of endocoronary prostheses.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
26

Data source: Europe PubMed Central

Abstract
Intracoronary stenting has markedly improved the patency of native coronary arteries after percutaneous transluminal coronary angioplasty (PTCA). Advances in stent technology and design, including drug releasing stents, have contributed to reduce the long-term restenosis rate. However, stenosis caused by neointimal hyperplasia, vascular remodeling and thrombosis is still a major problem after endocoronary stent procedures. This study focuses on differential gene expression of circulating peripheral blood cells after 90 min exposure to stents to search for initially activated cellular pathways, which may foster restenosis. Fresh human whole blood (1 IU heparin/ml), taken from non-medicated healthy volunteers, was incubated under flow conditions in an in vitro closed-loop stent-testing model (modified Chandler-Loop). Differential gene expression compared to resting conditions and to the experimental controls was investigated by a DNA-microarray technique encoding for over 17,000 genes simultaneously. As expected, a large variety of genes showed differential gene expression. Interestingly, Thrombospondin 1 (TSP-1), which plays a key role in initial immune defense, was found to be the most markedly up-regulated gene. We propose TSP-1 expression as an early indicator for the activation of immune responses following intracoronary stenting. After clarifying the participation of TSP-1 in vivo, future studies will therefore focus on TSP-1 as a potential prognostic factor, which may also help to develop and control new surface materials with an improved biocompatibility.
Date of acceptance
2005
Autoren
  • Jan Hoffmann
  • Perikles Simon
  • Anja K Zimmermann
  • Michael Lemancyk
  • Thomas Walter
  • Martin Beyer
  • Hans-Martin Hoffmeister
  • Gerhard Ziemer
  • Hans P Wendel
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/15792551
DOI
10.1016/j.biomaterials.2005.01.016
ISSN
0142-9612
Ausgabe der Veröffentlichung
25
Zeitschrift
Biomaterials
Schlüsselwörter
  • Adult
  • Biocompatible Materials
  • Coronary Restenosis
  • Down-Regulation
  • Gene Expression
  • Gene Expression Profiling
  • Humans
  • In Vitro Techniques
  • Middle Aged
  • Models, Biological
  • Oligonucleotide Array Sequence Analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stents
  • Thrombospondin 1
  • Up-Regulation
Sprache
eng
Country
Netherlands
Paginierung
5240 - 5250
PII
S0142-9612(05)00042-6
Datum der Veröffentlichung
2005
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2005
Titel
Thrombospondin 1 as possible key factor in the hemocompatibility of endocoronary prostheses.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
26

Data source: PubMed

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