Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Anne Jantschke
- Eline Koers
- Deni Mance
- Markus Weingarth
- Eike Brunner
- Marc Baldus
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000368057400011&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1002/anie.201507327
- eISSN
- 1521-3773
- Externe Identifier
- Clarivate Analytics Document Solution ID: DA8LX
- PubMed Identifier: 26509491
- ISSN
- 1433-7851
- Ausgabe der Veröffentlichung
- 50
- Zeitschrift
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
- Schlüsselwörter
- biomineralization
- diatoms
- DNP
- hybrid materials
- solid-state NMR
- Paginierung
- 15069 - 15073
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Titel
- Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy
- Sub types
- Article
- Ausgabe der Zeitschrift
- 54
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer scale has so far remained unknown, in particular for intact biosilica. A DNP‐supported ssNMR approach assisted by microscopy, MS, and MD simulations was applied to study the structural organization of intact biosilica. For the first time, the secondary structure elements of tightly biosilica‐associated native proteins in diatom biosilica were characterized in situ. Our data suggest that these proteins are rich in a limited set of amino acids and adopt a mixture of random‐coil and β‐strand conformations. Furthermore, biosilica‐associated long‐chain polyamines and carbohydrates were characterized, thereby leading to a model for the supramolecular organization of intact biosilica.</jats:p>
- Autoren
- Anne Jantschke
- Eline Koers
- Deni Mance
- Markus Weingarth
- Eike Brunner
- Marc Baldus
- DOI
- 10.1002/anie.201507327
- eISSN
- 1521-3773
- ISSN
- 1433-7851
- Ausgabe der Veröffentlichung
- 50
- Zeitschrift
- Angewandte Chemie International Edition
- Sprache
- en
- Online publication date
- 2015
- Paginierung
- 15069 - 15073
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/anie.201507327
- Datum der Datenerfassung
- 2023
- Titel
- Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP‐Supported Solid‐State NMR Spectroscopy
- Ausgabe der Zeitschrift
- 54
Data source: Crossref
- Abstract
- Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer scale has so far remained unknown, in particular for intact biosilica. A DNP-supported ssNMR approach assisted by microscopy, MS, and MD simulations was applied to study the structural organization of intact biosilica. For the first time, the secondary structure elements of tightly biosilica-associated native proteins in diatom biosilica were characterized in situ. Our data suggest that these proteins are rich in a limited set of amino acids and adopt a mixture of random-coil and β-strand conformations. Furthermore, biosilica-associated long-chain polyamines and carbohydrates were characterized, thereby leading to a model for the supramolecular organization of intact biosilica.
- Addresses
- Bioanalytical Chemistry, TU Dresden, Bergstraße 66, 01069 Dresden (Germany).
- Autoren
- Anne Jantschke
- Eline Koers
- Deni Mance
- Markus Weingarth
- Eike Brunner
- Marc Baldus
- DOI
- 10.1002/anie.201507327
- eISSN
- 1521-3773
- Externe Identifier
- PubMed Identifier: 26509491
- Funding acknowledgements
- Dutch Research Council (NWO): 722.012.002
- Dutch Research Council (NWO): 700.11.344
- Dutch Research Council (NWO): 700.26.121
- Deutsche Forschungsgemeinschaft: BR 1278/24-1
- Open access
- false
- ISSN
- 1433-7851
- Ausgabe der Veröffentlichung
- 50
- Zeitschrift
- Angewandte Chemie (International ed. in English)
- Schlüsselwörter
- Diatoms
- Silicon Dioxide
- Macromolecular Substances
- Magnetic Resonance Spectroscopy
- Particle Size
- Surface Properties
- Reference Standards
- Molecular Dynamics Simulation
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2015
- Paginierung
- 15069 - 15073
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum der Datenerfassung
- 2015
- Titel
- Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 54
Data source: Europe PubMed Central
- Abstract
- Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer scale has so far remained unknown, in particular for intact biosilica. A DNP-supported ssNMR approach assisted by microscopy, MS, and MD simulations was applied to study the structural organization of intact biosilica. For the first time, the secondary structure elements of tightly biosilica-associated native proteins in diatom biosilica were characterized in situ. Our data suggest that these proteins are rich in a limited set of amino acids and adopt a mixture of random-coil and β-strand conformations. Furthermore, biosilica-associated long-chain polyamines and carbohydrates were characterized, thereby leading to a model for the supramolecular organization of intact biosilica.
- Autoren
- Anne Jantschke
- Eline Koers
- Deni Mance
- Markus Weingarth
- Eike Brunner
- Marc Baldus
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/26509491
- DOI
- 10.1002/anie.201507327
- eISSN
- 1521-3773
- Ausgabe der Veröffentlichung
- 50
- Zeitschrift
- Angew Chem Int Ed Engl
- Schlüsselwörter
- DNP
- biomineralization
- diatoms
- hybrid materials
- solid-state NMR
- Diatoms
- Macromolecular Substances
- Magnetic Resonance Spectroscopy
- Molecular Dynamics Simulation
- Particle Size
- Reference Standards
- Silicon Dioxide
- Surface Properties
- Sprache
- eng
- Country
- Germany
- Paginierung
- 15069 - 15073
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2016
- Titel
- Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 54
Data source: PubMed
- Beziehungen:
- Property of