Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices

Publication type:
Journal article
Metadata:
Autoren
  • AM Pabst
  • W Wagner
  • A Kasaj
  • S Gebhardt
  • M Ackermann
  • A Astolfo
  • F Marone
  • D Haberthuer
  • F Enzmann
  • Moritz A Konerding
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000350353300046&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1007/s00784-014-1312-4
eISSN
1436-3771
Externe Identifier
  • Clarivate Analytics Document Solution ID: CC4VT
  • PubMed Identifier: 25209594
ISSN
1432-6981
Ausgabe der Veröffentlichung
2
Zeitschrift
CLINICAL ORAL INVESTIGATIONS
Schlüsselwörter
  • Synchrotron radiation
  • 3D visualization
  • Morphometry
  • Collagen matrix
Paginierung
561 - 564
Datum der Veröffentlichung
2015
Status
Published
Titel
Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices
Sub types
  • Article
Ausgabe der Zeitschrift
19

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • AM Pabst
  • W Wagner
  • A Kasaj
  • S Gebhardt
  • M Ackermann
  • A Astolfo
  • F Marone
  • D Haberthür
  • F Enzmann
  • Moritz A Konerding
DOI
10.1007/s00784-014-1312-4
eISSN
1436-3771
ISSN
1432-6981
Ausgabe der Veröffentlichung
2
Zeitschrift
Clinical Oral Investigations
Sprache
en
Online publication date
2014
Paginierung
561 - 564
Datum der Veröffentlichung
2015
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1007/s00784-014-1312-4
Datum der Datenerfassung
2019
Titel
Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices
Ausgabe der Zeitschrift
19

Data source: Crossref

Abstract
<h4>Introduction</h4>Three-dimensional collagen matrices (3D-CMs) may be visualized by cumbersome reconstructions of serial sections. We report here on the method of synchrotron-based X-ray tomographic microscopy (SRXTM) to image 3D-CMs in native tissue probes.<h4>Material and methods</h4>SRXTM of 3D-CMs (mucoderm®, mucograft®) was performed at the TOMCAT beamline of the Swiss Light Source (SLS) at the Paul Scherrer Institute (Villigen, Switzerland).<h4>Results</h4>SRXTM combines the advantages of high-resolution scanning electron microscopy (SEM) imaging with the low-resolution reconstructions of micro-CT (μCT) imaging. It may be used to non-destructively visualize and analyze structures within the 3D-CMs without the need of serial sectioning and reconstruction.<h4>Conclusion</h4>High-resolution SRXTM is a useful tool in analyzing the topology and morphometry of structures in 3D-CMs. The outcome justifies the efforts in sophisticated data processing.<h4>Clinical relevance</h4>SRXTM may help to understand the clinical characteristics of 3D-CMs in more detail.
Addresses
  • Institute of Functional and Clinical Anatomy, University Medical Center Mainz, Becherweg 13, 55099, Mainz, Germany.
Autoren
  • AM Pabst
  • W Wagner
  • A Kasaj
  • S Gebhardt
  • M Ackermann
  • A Astolfo
  • F Marone
  • D Haberthür
  • F Enzmann
  • Moritz A Konerding
DOI
10.1007/s00784-014-1312-4
eISSN
1436-3771
Externe Identifier
  • PubMed Identifier: 25209594
Open access
false
ISSN
1432-6981
Ausgabe der Veröffentlichung
2
Zeitschrift
Clinical oral investigations
Schlüsselwörter
  • Collagen
  • Tomography, X-Ray
  • Synchrotrons
Sprache
eng
Medium
Print-Electronic
Online publication date
2014
Paginierung
561 - 564
Datum der Veröffentlichung
2015
Status
Published
Datum der Datenerfassung
2014
Titel
Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
19

Data source: Europe PubMed Central

Abstract
INTRODUCTION: Three-dimensional collagen matrices (3D-CMs) may be visualized by cumbersome reconstructions of serial sections. We report here on the method of synchrotron-based X-ray tomographic microscopy (SRXTM) to image 3D-CMs in native tissue probes. MATERIAL AND METHODS: SRXTM of 3D-CMs (mucoderm®, mucograft®) was performed at the TOMCAT beamline of the Swiss Light Source (SLS) at the Paul Scherrer Institute (Villigen, Switzerland). RESULTS: SRXTM combines the advantages of high-resolution scanning electron microscopy (SEM) imaging with the low-resolution reconstructions of micro-CT (μCT) imaging. It may be used to non-destructively visualize and analyze structures within the 3D-CMs without the need of serial sectioning and reconstruction. CONCLUSION: High-resolution SRXTM is a useful tool in analyzing the topology and morphometry of structures in 3D-CMs. The outcome justifies the efforts in sophisticated data processing. CLINICAL RELEVANCE: SRXTM may help to understand the clinical characteristics of 3D-CMs in more detail.
Date of acceptance
2014
Autoren
  • AM Pabst
  • W Wagner
  • A Kasaj
  • S Gebhardt
  • M Ackermann
  • A Astolfo
  • F Marone
  • D Haberthür
  • F Enzmann
  • Moritz A Konerding
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/25209594
DOI
10.1007/s00784-014-1312-4
eISSN
1436-3771
Ausgabe der Veröffentlichung
2
Zeitschrift
Clin Oral Investig
Schlüsselwörter
  • Collagen
  • Synchrotrons
  • Tomography, X-Ray
Sprache
eng
Country
Germany
Paginierung
561 - 564
Datum der Veröffentlichung
2015
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
19

Data source: PubMed

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