Modulation of Intestinal Corticotropin-Releasing Hormone Signaling by the Herbal Preparation STW 5-II: Possible Mechanisms for Irritable Bowel Syndrome Management
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Mohamed Elbadawi
- Ramy M Ammar
- Sabine Rabini
- Sabine M Klauck
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000857003300001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/ph15091121
- eISSN
- 1424-8247
- Externe Identifier
- Clarivate Analytics Document Solution ID: 4R8JO
- PubMed Identifier: 36145342
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- PHARMACEUTICALS
- Schlüsselwörter
- irritable bowel syndrome
- mouse intestinal organoids
- corticotropin-releasing factor
- gut-brain axis
- tight junctions
- intestinal inflammation
- proinflammatory cytokines
- stress
- Artikelnummer
- ARTN 1121
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Titel
- Modulation of Intestinal Corticotropin-Releasing Hormone Signaling by the Herbal Preparation STW 5-II: Possible Mechanisms for Irritable Bowel Syndrome Management
- Sub types
- Article
- Ausgabe der Zeitschrift
- 15
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p>Corticotropin-releasing factor (CRF) mediates stress responses and alters the gut-brain axis, contributing to the pathogenesis of irritable bowel syndrome (IBS), which is recognized by abdominal pain accompanied by bowel habit disturbance. STW 5-II, a mixture of six herbal extracts, is clinically effective in functional dyspepsia and IBS. Here we aimed to establish an organoid-based stress-induced IBS-like model to investigate the mechanisms of action of STW 5-II. STW 5-II (10, 20, and 30 g/mL) was applied to intestinal organoids for 24 h before being treated with CRF (100 nM) for 48 h. The effects of STW 5-II on CRF signaling were investigated using several in vitro and in silico approaches. STW 5-II activities were further explored by in silico PyRx screening followed by molecular docking of the main 52 identified compounds in STW 5-II with both CRF receptors CRFR1 and CRFR2. CRF exposure stimulated inflammation and increased proinflammatory mediators, while STW 5-II dose-dependently counteracted these effects. STW 5-II inhibited CRF-induced claudin-2 overexpression and serotonin release. Docking of the STW 5-II constituents oleanolic acid and licorice saponin G2 to CRFR1 and CRFR2, respectively, showed a good affinity. These multi-target activities support and elucidate the clinically proven efficacy of STW 5-II in disorders of gut-brain interaction.</jats:p>
- Autoren
- Mohamed Elbadawi
- Ramy Ammar
- Sabine Rabini
- Sabine Klauck
- Thomas Efferth
- DOI
- 10.3390/ph15091121
- eISSN
- 1424-8247
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Pharmaceuticals
- Sprache
- en
- Online publication date
- 2022
- Paginierung
- 1121 - 1121
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/ph15091121
- Datum der Datenerfassung
- 2022
- Titel
- Modulation of Intestinal Corticotropin-Releasing Hormone Signaling by the Herbal Preparation STW 5-II: Possible Mechanisms for Irritable Bowel Syndrome Management
- Ausgabe der Zeitschrift
- 15
Data source: Crossref
- Abstract
- Corticotropin-releasing factor (CRF) mediates stress responses and alters the gut-brain axis, contributing to the pathogenesis of irritable bowel syndrome (IBS), which is recognized by abdominal pain accompanied by bowel habit disturbance. STW 5-II, a mixture of six herbal extracts, is clinically effective in functional dyspepsia and IBS. Here we aimed to establish an organoid-based stress-induced IBS-like model to investigate the mechanisms of action of STW 5-II. STW 5-II (10, 20, and 30 g/mL) was applied to intestinal organoids for 24 h before being treated with CRF (100 nM) for 48 h. The effects of STW 5-II on CRF signaling were investigated using several in vitro and in silico approaches. STW 5-II activities were further explored by in silico PyRx screening followed by molecular docking of the main 52 identified compounds in STW 5-II with both CRF receptors CRFR1 and CRFR2. CRF exposure stimulated inflammation and increased proinflammatory mediators, while STW 5-II dose-dependently counteracted these effects. STW 5-II inhibited CRF-induced claudin-2 overexpression and serotonin release. Docking of the STW 5-II constituents oleanolic acid and licorice saponin G2 to CRFR1 and CRFR2, respectively, showed a good affinity. These multi-target activities support and elucidate the clinically proven efficacy of STW 5-II in disorders of gut-brain interaction.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University-Mainz, 55128 Mainz, Germany.
- Autoren
- Mohamed Elbadawi
- Ramy M Ammar
- Sabine Rabini
- Sabine M Klauck
- Thomas Efferth
- DOI
- 10.3390/ph15091121
- eISSN
- 1424-8247
- Externe Identifier
- PubMed Identifier: 36145342
- PubMed Central ID: PMC9504045
- Funding acknowledgements
- Bayer Consumer Health, Darmstadt, Germany: NA
- Open access
- true
- ISSN
- 1424-8247
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Pharmaceuticals (Basel, Switzerland)
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2022
- Open access status
- Open Access
- Paginierung
- 1121
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2022
- Titel
- Modulation of Intestinal Corticotropin-Releasing Hormone Signaling by the Herbal Preparation STW 5-II: Possible Mechanisms for Irritable Bowel Syndrome Management.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 15
Files
https://www.mdpi.com/1424-8247/15/9/1121/pdf?version=1662626160 https://europepmc.org/articles/PMC9504045?pdf=render
Data source: Europe PubMed Central
- Abstract
- Corticotropin-releasing factor (CRF) mediates stress responses and alters the gut-brain axis, contributing to the pathogenesis of irritable bowel syndrome (IBS), which is recognized by abdominal pain accompanied by bowel habit disturbance. STW 5-II, a mixture of six herbal extracts, is clinically effective in functional dyspepsia and IBS. Here we aimed to establish an organoid-based stress-induced IBS-like model to investigate the mechanisms of action of STW 5-II. STW 5-II (10, 20, and 30 g/mL) was applied to intestinal organoids for 24 h before being treated with CRF (100 nM) for 48 h. The effects of STW 5-II on CRF signaling were investigated using several in vitro and in silico approaches. STW 5-II activities were further explored by in silico PyRx screening followed by molecular docking of the main 52 identified compounds in STW 5-II with both CRF receptors CRFR1 and CRFR2. CRF exposure stimulated inflammation and increased proinflammatory mediators, while STW 5-II dose-dependently counteracted these effects. STW 5-II inhibited CRF-induced claudin-2 overexpression and serotonin release. Docking of the STW 5-II constituents oleanolic acid and licorice saponin G2 to CRFR1 and CRFR2, respectively, showed a good affinity. These multi-target activities support and elucidate the clinically proven efficacy of STW 5-II in disorders of gut-brain interaction.
- Date of acceptance
- 2022
- Autoren
- Mohamed Elbadawi
- Ramy M Ammar
- Sabine Rabini
- Sabine M Klauck
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/36145342
- DOI
- 10.3390/ph15091121
- Externe Identifier
- PubMed Central ID: PMC9504045
- Funding acknowledgements
- Bayer Consumer Health, Darmstadt, Germany: NA
- ISSN
- 1424-8247
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Pharmaceuticals (Basel)
- Schlüsselwörter
- corticotropin-releasing factor
- gut-brain axis
- intestinal inflammation
- irritable bowel syndrome
- mouse intestinal organoids
- proinflammatory cytokines
- stress
- tight junctions
- Sprache
- eng
- Country
- Switzerland
- PII
- ph15091121
- Datum der Veröffentlichung
- 2022
- Status
- Published online
- Titel
- Modulation of Intestinal Corticotropin-Releasing Hormone Signaling by the Herbal Preparation STW 5-II: Possible Mechanisms for Irritable Bowel Syndrome Management.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 15
Data source: PubMed
- Beziehungen:
-