Cytotoxicity, acute and sub-chronic toxicities of the fruit extract of Tetrapleura tetraptera (Schumm. & Thonn.) Taub. (Fabaceae)
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Idrios N Bonsou
- Armelle T Mbaveng
- Gaelle S Nguenang
- Godloves F Chi
- Thomas Efferth
- Victor Kuete
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000820628100001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1186/s12906-022-03659-1
- eISSN
- 2662-7671
- Externe Identifier
- Clarivate Analytics Document Solution ID: 2Q7VS
- PubMed Identifier: 35787267
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC COMPLEMENTARY MEDICINE AND THERAPIES
- Schlüsselwörter
- Cytotoxicity
- Fabaceae
- Spice
- Tetrapleura tetraptera
- Toxicity
- Artikelnummer
- ARTN 178
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Titel
- Cytotoxicity, acute and sub-chronic toxicities of the fruit extract of <i>Tetrapleura tetraptera (Schumm. & Thonn.) Taub.</i> (Fabaceae)
- Sub types
- Article
- Ausgabe der Zeitschrift
- 22
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p><jats:italic>Tetrapleura tetraptera</jats:italic> is a medicinal spice traditionally used to treat cancer, diabetes, and several other ailments. This study analyzed the cytotoxicity of the dichloromethane methanol extract of <jats:italic>T. tetraptera</jats:italic> fruits (TTF) and its constituents. The toxicity profile of the TTF extract was also evaluated in rats.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>The Cytotoxicity of this extract was evaluated using the resazurin reduction assay (RRA). Acute and sub-chronic toxicity studies were performed according to the protocol described by the Organisation for Economic Cooperation, and Development (OECD). Hematological, serum, and urine biochemical parameters, as well as histological sections of the liver and kidney, were also evaluated based on standard methods.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>The TTF extract, compound 5, and the reference drug doxorubicin were active in all 9 tested cancer cell lines. The recorded IC<jats:sub>50</jats:sub> ranged from 18.32 μM (against B16-F1 murine melanoma cells) to 36.18 μM (against SKMel-505 BRAF wildtype melanoma cells) for TTF, from 10.02 μM (towards MaMel-80a BRAF-V600E homozygous mutant melanoma cells) to 31.73 μM (against SKMel-28 BRAF-V600E homozygous mutant melanoma cells) for compound 5, and from 0.22 μM (against B16-F1 cells) to 9.39 μM (against SKMel-505 cells) for doxorubicin. The study of acute toxicity test showed that the lethal dose (LD<jats:sub>50</jats:sub>) of this extract was greater than 5000 mg/kg body weight. In the sub-chronic toxicity studies, variations were observed in some biochemical parameters, especially at higher doses.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>TTF and its most active compound (5) are found to be potential cytotoxic agents, meanwhile, TTF was safe when given a single oral dose of 5000 mg/kg. However, caution is necessary in case of prolonged oral administration due to potential alterations of renal function at high doses (> 1000 mg/kg).</jats:p> </jats:sec>
- Autoren
- Idrios N Bonsou
- Armelle T Mbaveng
- Gaëlle S Nguenang
- Godloves F Chi
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1186/s12906-022-03659-1
- eISSN
- 2662-7671
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC Complementary Medicine and Therapies
- Sprache
- en
- Artikelnummer
- 178
- Online publication date
- 2022
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1186/s12906-022-03659-1
- Datum der Datenerfassung
- 2022
- Titel
- Cytotoxicity, acute and sub-chronic toxicities of the fruit extract of Tetrapleura tetraptera (Schumm. & Thonn.) Taub. (Fabaceae)
- Ausgabe der Zeitschrift
- 22
Data source: Crossref
- Abstract
- <h4>Background</h4>Tetrapleura tetraptera is a medicinal spice traditionally used to treat cancer, diabetes, and several other ailments. This study analyzed the cytotoxicity of the dichloromethane methanol extract of T. tetraptera fruits (TTF) and its constituents. The toxicity profile of the TTF extract was also evaluated in rats.<h4>Methods</h4>The Cytotoxicity of this extract was evaluated using the resazurin reduction assay (RRA). Acute and sub-chronic toxicity studies were performed according to the protocol described by the Organisation for Economic Cooperation, and Development (OECD). Hematological, serum, and urine biochemical parameters, as well as histological sections of the liver and kidney, were also evaluated based on standard methods.<h4>Results</h4>The TTF extract, compound 5, and the reference drug doxorubicin were active in all 9 tested cancer cell lines. The recorded IC<sub>50</sub> ranged from 18.32 μM (against B16-F1 murine melanoma cells) to 36.18 μM (against SKMel-505 BRAF wildtype melanoma cells) for TTF, from 10.02 μM (towards MaMel-80a BRAF-V600E homozygous mutant melanoma cells) to 31.73 μM (against SKMel-28 BRAF-V600E homozygous mutant melanoma cells) for compound 5, and from 0.22 μM (against B16-F1 cells) to 9.39 μM (against SKMel-505 cells) for doxorubicin. The study of acute toxicity test showed that the lethal dose (LD<sub>50</sub>) of this extract was greater than 5000 mg/kg body weight. In the sub-chronic toxicity studies, variations were observed in some biochemical parameters, especially at higher doses.<h4>Conclusion</h4>TTF and its most active compound (5) are found to be potential cytotoxic agents, meanwhile, TTF was safe when given a single oral dose of 5000 mg/kg. However, caution is necessary in case of prolonged oral administration due to potential alterations of renal function at high doses (> 1000 mg/kg).
- Addresses
- Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
- Autoren
- Idrios N Bonsou
- Armelle T Mbaveng
- Gaëlle S Nguenang
- Godloves F Chi
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1186/s12906-022-03659-1
- eISSN
- 2662-7671
- Externe Identifier
- PubMed Identifier: 35787267
- PubMed Central ID: PMC9252075
- Open access
- true
- ISSN
- 2662-7671
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC complementary medicine and therapies
- Schlüsselwörter
- Animals
- Mice
- Rats
- Tetrapleura
- Fruit
- Melanoma
- Doxorubicin
- Proto-Oncogene Proteins B-raf
- Plant Extracts
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2022
- Open access status
- Open Access
- Paginierung
- 178
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2022
- Titel
- Cytotoxicity, acute and sub-chronic toxicities of the fruit extract of Tetrapleura tetraptera (Schumm. & Thonn.) Taub. (Fabaceae).
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 22
Files
https://bmccomplementmedtherapies.biomedcentral.com/counter/pdf/10.1186/s12906-022-03659-1 https://europepmc.org/articles/PMC9252075?pdf=render
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Tetrapleura tetraptera is a medicinal spice traditionally used to treat cancer, diabetes, and several other ailments. This study analyzed the cytotoxicity of the dichloromethane methanol extract of T. tetraptera fruits (TTF) and its constituents. The toxicity profile of the TTF extract was also evaluated in rats. METHODS: The Cytotoxicity of this extract was evaluated using the resazurin reduction assay (RRA). Acute and sub-chronic toxicity studies were performed according to the protocol described by the Organisation for Economic Cooperation, and Development (OECD). Hematological, serum, and urine biochemical parameters, as well as histological sections of the liver and kidney, were also evaluated based on standard methods. RESULTS: The TTF extract, compound 5, and the reference drug doxorubicin were active in all 9 tested cancer cell lines. The recorded IC50 ranged from 18.32 μM (against B16-F1 murine melanoma cells) to 36.18 μM (against SKMel-505 BRAF wildtype melanoma cells) for TTF, from 10.02 μM (towards MaMel-80a BRAF-V600E homozygous mutant melanoma cells) to 31.73 μM (against SKMel-28 BRAF-V600E homozygous mutant melanoma cells) for compound 5, and from 0.22 μM (against B16-F1 cells) to 9.39 μM (against SKMel-505 cells) for doxorubicin. The study of acute toxicity test showed that the lethal dose (LD50) of this extract was greater than 5000 mg/kg body weight. In the sub-chronic toxicity studies, variations were observed in some biochemical parameters, especially at higher doses. CONCLUSION: TTF and its most active compound (5) are found to be potential cytotoxic agents, meanwhile, TTF was safe when given a single oral dose of 5000 mg/kg. However, caution is necessary in case of prolonged oral administration due to potential alterations of renal function at high doses (> 1000 mg/kg).
- Date of acceptance
- 2022
- Autoren
- Idrios N Bonsou
- Armelle T Mbaveng
- Gaëlle S Nguenang
- Godloves F Chi
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/35787267
- DOI
- 10.1186/s12906-022-03659-1
- eISSN
- 2662-7671
- Externe Identifier
- PubMed Central ID: PMC9252075
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC Complement Med Ther
- Schlüsselwörter
- Cytotoxicity
- Fabaceae
- Spice
- Tetrapleura tetraptera
- Toxicity
- Animals
- Doxorubicin
- Fruit
- Melanoma
- Mice
- Plant Extracts
- Proto-Oncogene Proteins B-raf
- Rats
- Tetrapleura
- Sprache
- eng
- Country
- England
- Paginierung
- 178
- PII
- 10.1186/s12906-022-03659-1
- Datum der Veröffentlichung
- 2022
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Titel
- Cytotoxicity, acute and sub-chronic toxicities of the fruit extract of Tetrapleura tetraptera (Schumm. & Thonn.) Taub. (Fabaceae).
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 22
Data source: PubMed
- Beziehungen:
- Property of