Expression of the Stem Cell Marker ABCB5 in Normal and Tumor Tissues

Publication type:
Journal article
Metadata:
Autoren
  • Mohamed EM Saeed
  • Joelle C Boulos
  • Kevin Machel
  • Nasim Andabili
  • Thamail Marouni
  • Wilfried Roth
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000818925800012&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.21873/invivo.12877
eISSN
1791-7549
Externe Identifier
  • Clarivate Analytics Document Solution ID: 2O2WV
  • PubMed Identifier: 35738589
ISSN
0258-851X
Ausgabe der Veröffentlichung
4
Zeitschrift
IN VIVO
Schlüsselwörter
  • Key Words
  • Chemotherapy
  • drug resistance
  • histology
  • stem cell marker
  • xenobiotic detoxification
Paginierung
1651 - 1666
Datum der Veröffentlichung
2022
Status
Published
Titel
Expression of the Stem Cell Marker ABCB5 in Normal and Tumor Tissues
Sub types
  • Article
Ausgabe der Zeitschrift
36

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • MOHAMED EM SAEED
  • JOELLE C BOULOS
  • KEVIN MACHEL
  • NASIM ANDABILI
  • THAMAIL MAROUNI
  • WILFRIED ROTH
  • THOMAS EFFERTH
DOI
10.21873/invivo.12877
eISSN
1791-7549
ISSN
0258-851X
Ausgabe der Veröffentlichung
4
Zeitschrift
In Vivo
Sprache
en
Online publication date
2022
Paginierung
1651 - 1666
Datum der Veröffentlichung
2022
Status
Published
Herausgeber
Anticancer Research USA Inc.
Herausgeber URL
http://dx.doi.org/10.21873/invivo.12877
Datum der Datenerfassung
2023
Titel
Expression of the Stem Cell Marker ABCB5 in Normal and Tumor Tissues
Ausgabe der Zeitschrift
36

Data source: Crossref

Abstract
<h4>Background/aim</h4>The ATP-binding cassette subfamily B member 5 (ABCB5) transporter plays a pivotal role in melanocyte progenitor cell fusion and has been identified as a tumor-initiating cell marker. In this study, we determined ABCB5 expression in normal tissues among various species, i.e., Homo sapiens, Mus musculus (mouse), Rattus norvegicus (rat), Sus scrofa domesticus (pig), Gallus gallus (chicken), Anser anser (goose), Poecilia reticulata (Guppy fish), and Lumbricus terrestris (earthworm), as well as 426 biopsies of different human tumor types (colorectal, cervical, endometrium, vaginal, nasopharyngeal, kidney, breast, colon, prostate, pancreas, lung, gallbladder, bladder, brain, liver, skin, small intestine, testis, tonsil, uterus, thyroid, stomach, esophagus, fallopian, parotid, and ovary).<h4>Materials and methods</h4>Using immunohistochemical staining, ABCB5 expression was detected and evaluated in formalin-fixed, paraffin-embedded sections.<h4>Results</h4>High ABCB5 expression was found in normal tissues in specialized cells with secretory and excretory functions, chorionic villi of the placenta, hepatocytes, and blood-tissue barrier sites in the brain and testis. Besides, heterogeneous expression of ABCB5 was also observed in many different tumor types derived from breast, endometrium, ovary, uterus, cervix, prostate, lung, brain, colon, liver, nasopharynx, and others.<h4>Conclusion</h4>The localization of ABCB5 in different normal tissues suggests that this protein has an excretory pumping role for physiological metabolites and xenobiotics. This physiological role highlighted its possible impact on the development of multidrug resistance in tumors. Further studies are required to establish the possible clinical significance of ABCB5 as a predictive marker for drug resistance and as a prognostic marker for patient survival.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Mainz, Germany.
Autoren
  • Mohamed EM Saeed
  • Joelle C Boulos
  • Kevin Machel
  • Nasim Andabili
  • Thamail Marouni
  • Wilfried Roth
  • Thomas Efferth
DOI
10.21873/invivo.12877
eISSN
1791-7549
Externe Identifier
  • PubMed Identifier: 35738589
  • PubMed Central ID: PMC9301431
Open access
false
ISSN
0258-851X
Ausgabe der Veröffentlichung
4
Zeitschrift
In vivo (Athens, Greece)
Schlüsselwörter
  • Skin
  • Animals
  • Geese
  • Chickens
  • Swine
  • Humans
  • Mice
  • Rats
  • Oligochaeta
  • Pregnancy
  • Female
  • Male
  • Neoplastic Stem Cells
  • Biomarkers
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
Sprache
eng
Medium
Print
Paginierung
1651 - 1666
Datum der Veröffentlichung
2022
Status
Published
Datum der Datenerfassung
2022
Titel
Expression of the Stem Cell Marker ABCB5 in Normal and Tumor Tissues.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
36

Files

https://iv.iiarjournals.org/content/invivo/36/4/1651.full.pdf https://europepmc.org/articles/PMC9301431?pdf=render

Data source: Europe PubMed Central

Abstract
BACKGROUND/AIM: The ATP-binding cassette subfamily B member 5 (ABCB5) transporter plays a pivotal role in melanocyte progenitor cell fusion and has been identified as a tumor-initiating cell marker. In this study, we determined ABCB5 expression in normal tissues among various species, i.e., Homo sapiens, Mus musculus (mouse), Rattus norvegicus (rat), Sus scrofa domesticus (pig), Gallus gallus (chicken), Anser anser (goose), Poecilia reticulata (Guppy fish), and Lumbricus terrestris (earthworm), as well as 426 biopsies of different human tumor types (colorectal, cervical, endometrium, vaginal, nasopharyngeal, kidney, breast, colon, prostate, pancreas, lung, gallbladder, bladder, brain, liver, skin, small intestine, testis, tonsil, uterus, thyroid, stomach, esophagus, fallopian, parotid, and ovary). MATERIALS AND METHODS: Using immunohistochemical staining, ABCB5 expression was detected and evaluated in formalin-fixed, paraffin-embedded sections. RESULTS: High ABCB5 expression was found in normal tissues in specialized cells with secretory and excretory functions, chorionic villi of the placenta, hepatocytes, and blood-tissue barrier sites in the brain and testis. Besides, heterogeneous expression of ABCB5 was also observed in many different tumor types derived from breast, endometrium, ovary, uterus, cervix, prostate, lung, brain, colon, liver, nasopharynx, and others. CONCLUSION: The localization of ABCB5 in different normal tissues suggests that this protein has an excretory pumping role for physiological metabolites and xenobiotics. This physiological role highlighted its possible impact on the development of multidrug resistance in tumors. Further studies are required to establish the possible clinical significance of ABCB5 as a predictive marker for drug resistance and as a prognostic marker for patient survival.
Date of acceptance
2022
Autoren
  • Mohamed EM Saeed
  • Joelle C Boulos
  • Kevin Machel
  • Nasim Andabili
  • Thamail Marouni
  • Wilfried Roth
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/35738589
DOI
10.21873/invivo.12877
eISSN
1791-7549
Externe Identifier
  • PubMed Central ID: PMC9301431
Ausgabe der Veröffentlichung
4
Zeitschrift
In Vivo
Schlüsselwörter
  • Chemotherapy
  • drug resistance
  • histology
  • stem cell marker
  • xenobiotic detoxification
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Animals
  • Biomarkers
  • Chickens
  • Female
  • Geese
  • Humans
  • Male
  • Mice
  • Neoplastic Stem Cells
  • Oligochaeta
  • Pregnancy
  • Rats
  • Skin
  • Swine
Sprache
eng
Country
Greece
Paginierung
1651 - 1666
PII
36/4/1651
Datum der Veröffentlichung
2022
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Titel
Expression of the Stem Cell Marker ABCB5 in Normal and Tumor Tissues.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
36

Data source: PubMed

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