A saponin from astragalus promotes pancreatic ductal organoids differentiation into insulin-producing cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Wen Yu
- Yannan Wang
- Di Jiang
- Jie Shang
- Miao Liu
- Thomas Efferth
- Chun-Bo Teng
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000807207100003&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2022.154190
- eISSN
- 1618-095X
- Externe Identifier
- Clarivate Analytics Document Solution ID: 1X1FD
- PubMed Identifier: 35636173
- ISSN
- 0944-7113
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- Insulin-producing cells
- Diabetes mellitus
- Astragalus saponins
- Organoids
- Differentiation
- Artikelnummer
- ARTN 154190
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Titel
- A saponin from astragalus promotes pancreatic ductal organoids differentiation into insulin-producing cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 102
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Wen Yu
- Yannan Wang
- Di Jiang
- Jie Shang
- Miao Liu
- Thomas Efferth
- Chun-Bo Teng
- DOI
- 10.1016/j.phymed.2022.154190
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Artikelnummer
- 154190
- Paginierung
- 154190 - 154190
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2022.154190
- Datum der Datenerfassung
- 2024
- Titel
- A saponin from astragalus promotes pancreatic ductal organoids differentiation into insulin-producing cells
- Ausgabe der Zeitschrift
- 102
Data source: Crossref
- Abstract
- <h4>Background</h4>Islet transplantation is an effective treatment for the type 1 and severe type 2 diabetes, but it is restricted by the severe lack of pancreas donors. In vitro differentiation of pancreatic progenitors into insulin-secreting cells is one of the hopeful strategies in the cell transplantation therapy of diabetes. Isoastragaloside I is one of the saponin molecules found in Astragalus membranaceus, which has been demonstrated to alleviate insulin resistance and glucose intolerance in obese mice.<h4>Study design</h4>We established mouse pancreatic ductal organoids (mPDOs) with progenitor characteristics and an insulin promoter-driven EGFP reporter system to screen astragalus saponin components for monomers that can promote insulin-producing cell differentiation.<h4>Methods</h4>mPDOs treated with or without astragalus saponin monomers were investigated by the insulin promoter-driven EGFP reporter, quantitative PCR, immunofluorescence and flow cytometry to evaluate the expression of endocrine progenitor and β-cell markers.<h4>Results</h4>Isoastragaloside I significantly promoted the expression of β-cell differentiation genes, which was demonstrated by the activation of the insulin promoter-driven EGFP reporter, as well as the significant increase of mRNA levels of the endocrine progenitor marker Ngn3 and the β-cell markers insulin1 and insulin2. Immunostaining studies indicated that the β-cell-specific C-peptide was upregulated in isoastragaloside I-treated mPDOs. FACS analysis revealed that the ratio of C-peptide-secreting cells in isoastragaloside I-treated mPDOs was over 40%. Glucose tolerance tests demonstrated that the differentiated mPDOs could secrete C-peptide in response to glucose stimulation.<h4>Conclusions</h4>We discover a novel strategy of inducing pancreatic ductal progenitors to differentiate into insulin-producing cells using isoastragaloside I. This approach can be potentially applied to β-cell transplantation in diabetes therapies.
- Addresses
- College of Life Science, Northeast Forestry University, Harbin, PR China.
- Autoren
- Wen Yu
- Yannan Wang
- Di Jiang
- Jie Shang
- Miao Liu
- Thomas Efferth
- Chun-Bo Teng
- DOI
- 10.1016/j.phymed.2022.154190
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 35636173
- Funding acknowledgements
- Fundamental Research Funds for the Central Universities:
- National Natural Science Foundation of China: 32072801
- Fundamental Research Funds for the Central Universities: 2572020AW36
- Fundamental Research Funds for the Central Universities: 2572020DY12
- Open access
- false
- ISSN
- 0944-7113
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Organoids
- Animals
- Mice
- Diabetes Mellitus, Type 2
- Insulin
- C-Peptide
- Saponins
- Cell Differentiation
- Insulin-Secreting Cells
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2022
- Paginierung
- 154190
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Datum der Datenerfassung
- 2022
- Titel
- A saponin from astragalus promotes pancreatic ductal organoids differentiation into insulin-producing cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 102
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Islet transplantation is an effective treatment for the type 1 and severe type 2 diabetes, but it is restricted by the severe lack of pancreas donors. In vitro differentiation of pancreatic progenitors into insulin-secreting cells is one of the hopeful strategies in the cell transplantation therapy of diabetes. Isoastragaloside I is one of the saponin molecules found in Astragalus membranaceus, which has been demonstrated to alleviate insulin resistance and glucose intolerance in obese mice. STUDY DESIGN: We established mouse pancreatic ductal organoids (mPDOs) with progenitor characteristics and an insulin promoter-driven EGFP reporter system to screen astragalus saponin components for monomers that can promote insulin-producing cell differentiation. METHODS: mPDOs treated with or without astragalus saponin monomers were investigated by the insulin promoter-driven EGFP reporter, quantitative PCR, immunofluorescence and flow cytometry to evaluate the expression of endocrine progenitor and β-cell markers. RESULTS: Isoastragaloside I significantly promoted the expression of β-cell differentiation genes, which was demonstrated by the activation of the insulin promoter-driven EGFP reporter, as well as the significant increase of mRNA levels of the endocrine progenitor marker Ngn3 and the β-cell markers insulin1 and insulin2. Immunostaining studies indicated that the β-cell-specific C-peptide was upregulated in isoastragaloside I-treated mPDOs. FACS analysis revealed that the ratio of C-peptide-secreting cells in isoastragaloside I-treated mPDOs was over 40%. Glucose tolerance tests demonstrated that the differentiated mPDOs could secrete C-peptide in response to glucose stimulation. CONCLUSIONS: We discover a novel strategy of inducing pancreatic ductal progenitors to differentiate into insulin-producing cells using isoastragaloside I. This approach can be potentially applied to β-cell transplantation in diabetes therapies.
- Date of acceptance
- 2022
- Autoren
- Wen Yu
- Yannan Wang
- Di Jiang
- Jie Shang
- Miao Liu
- Thomas Efferth
- Chun-Bo Teng
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/35636173
- DOI
- 10.1016/j.phymed.2022.154190
- eISSN
- 1618-095X
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- Astragalus saponins
- Diabetes mellitus
- Differentiation
- Insulin-producing cells
- Organoids
- Animals
- C-Peptide
- Cell Differentiation
- Diabetes Mellitus, Type 2
- Insulin
- Insulin-Secreting Cells
- Mice
- Organoids
- Saponins
- Sprache
- eng
- Country
- Germany
- Paginierung
- 154190
- PII
- S0944-7113(22)00268-9
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Titel
- A saponin from astragalus promotes pancreatic ductal organoids differentiation into insulin-producing cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 102
Data source: PubMed
- Beziehungen:
- Property of