Identification of active components in Andrographis paniculata targeting on CD81 in esophageal cancer in vitro and in vivo

Publication type:
Journal article
Metadata:
Autoren
  • Grace Gar-Lee Yue
  • Adele Joyce Gomes
  • Mohamed EM Saeed
  • Kei-Yin Tsui
  • Mona Dawood
  • Assia I Drif
  • Eric Chun-Wai Wong
  • Wai-Fung Lee
  • Wenjing Liu
  • Philip Wai-Yan Chiu
  • Thomas Efferth
  • Clara Bik-San Lau
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000820125300001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2022.154183
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: 2Q0ND
  • PubMed Identifier: 35636176
ISSN
0944-7113
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Andrographis paniculata
  • Esophageal cancer
  • CD81
  • Tetrasapanin
  • Metastasis
  • Bisandrographolide
Artikelnummer
ARTN 154183
Datum der Veröffentlichung
2022
Status
Published
Titel
Identification of active components in<i> Andrographis</i><i> paniculata</i> targeting on CD81 in esophageal cancer<i> in</i><i> vitro</i> and<i> in</i><i> vivo</i>
Sub types
  • Article
Ausgabe der Zeitschrift
102

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Grace Gar-Lee Yue
  • Adele Joyce Gomes
  • Mohamed EM Saeed
  • Kei-Yin Tsui
  • Mona Dawood
  • Assia I Drif
  • Eric Chun-Wai Wong
  • Wai-Fung Lee
  • Wenjing Liu
  • Philip Wai-Yan Chiu
  • Thomas Efferth
  • Clara Bik-San Lau
DOI
10.1016/j.phymed.2022.154183
ISSN
0944-7113
Zeitschrift
Phytomedicine
Sprache
en
Artikelnummer
154183
Paginierung
154183 - 154183
Datum der Veröffentlichung
2022
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2022.154183
Datum der Datenerfassung
2024
Titel
Identification of active components in Andrographis paniculata targeting on CD81 in esophageal cancer in vitro and in vivo
Ausgabe der Zeitschrift
102

Data source: Crossref

Abstract
<h4>Background</h4>Esophageal cancer (EC) is highly prevalent in Eastern Asia (including China) with high rates of mortality. The metastatic tendency in EC is associated with a poor prognosis. Our previous studies have demonstrated the suppressive effects of Andrographis paniculata water extract (APW) on metastatic esophageal cancer in vitro and in tumor-bearing mice models, as well as illustrated the potential underlying mechanism by transcriptome analysis.<h4>Hypothesis</h4>High expressions of several membrane protein tetraspanins were reported to lead to a high risk of metastasis in esophageal cancer in patients. We hypothesized that APW could downregulate the expression of tetraspanin CD81 in esophageal cancer cells and xenografts.<h4>Methods</h4>Human esophageal cancer cells EC109 and KYSE520 were incubated with APW for 24 hours in cell culture, while mice bearing EC109 xenograft tumors were treated with APW for 21 days. The expressions of CD81 in cancer cells and in tumors from mice were evaluated. Molecular docking and microscale thermophoresis analyses were applied to identify the components in APW interacting with CD81. The influence of the identified components on CD81 expression was further evaluated in EC109 cells.<h4>Results</h4>APW could significantly suppress the expressions of CD81 in both EC109 and KYSE520 cells in a concentration-dependent manner. Treatment of APW in xenograft-bearing mice reduces the metastasis in lungs, livers, and lymph nodes. The expression of CD81 in xenograft tumors of APW-treated mice was significantly lower than those of untreated control mice. The binding of andrographolide, bisandrographolide A, and bisandrographolide C with CD81 were elucidated by microscale thermophoresis. The suppressive effects of these compounds on the motility of EC109 cells, as well as CD81 protein and mRNA expressions, were further confirmed.<h4>Conclusion</h4>This is the first time to demonstrate that andrographolide, bisandrographolide A, and bisandrographolide C, which are present in APW, bind to CD81 and suppress its function. These compounds are likely to be responsible for the anti-metastatic activities of APW in esophageal cancer.
Addresses
  • Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
Autoren
  • Grace Gar-Lee Yue
  • Adele Joyce Gomes
  • Mohamed EM Saeed
  • Kei-Yin Tsui
  • Mona Dawood
  • Assia I Drif
  • Eric Chun-Wai Wong
  • Wai-Fung Lee
  • Wenjing Liu
  • Philip Wai-Yan Chiu
  • Thomas Efferth
  • Clara Bik-San Lau
DOI
10.1016/j.phymed.2022.154183
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 35636176
Funding acknowledgements
  • Innovation and Technology Fund: ITS/079/17FX
Open access
false
ISSN
0944-7113
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Animals
  • Humans
  • Mice
  • Esophageal Neoplasms
  • Diterpenes
  • Plant Extracts
  • Down-Regulation
  • Molecular Targeted Therapy
  • Molecular Docking Simulation
  • Tetraspanin 28
  • Andrographis paniculata
Sprache
eng
Medium
Print-Electronic
Online publication date
2022
Paginierung
154183
Datum der Veröffentlichung
2022
Status
Published
Datum der Datenerfassung
2022
Titel
Identification of active components in Andrographis paniculata targeting on CD81 in esophageal cancer in vitro and in vivo.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
102

Data source: Europe PubMed Central

Abstract
BACKGROUND: Esophageal cancer (EC) is highly prevalent in Eastern Asia (including China) with high rates of mortality. The metastatic tendency in EC is associated with a poor prognosis. Our previous studies have demonstrated the suppressive effects of Andrographis paniculata water extract (APW) on metastatic esophageal cancer in vitro and in tumor-bearing mice models, as well as illustrated the potential underlying mechanism by transcriptome analysis. HYPOTHESIS: High expressions of several membrane protein tetraspanins were reported to lead to a high risk of metastasis in esophageal cancer in patients. We hypothesized that APW could downregulate the expression of tetraspanin CD81 in esophageal cancer cells and xenografts. METHODS: Human esophageal cancer cells EC109 and KYSE520 were incubated with APW for 24 hours in cell culture, while mice bearing EC109 xenograft tumors were treated with APW for 21 days. The expressions of CD81 in cancer cells and in tumors from mice were evaluated. Molecular docking and microscale thermophoresis analyses were applied to identify the components in APW interacting with CD81. The influence of the identified components on CD81 expression was further evaluated in EC109 cells. RESULTS: APW could significantly suppress the expressions of CD81 in both EC109 and KYSE520 cells in a concentration-dependent manner. Treatment of APW in xenograft-bearing mice reduces the metastasis in lungs, livers, and lymph nodes. The expression of CD81 in xenograft tumors of APW-treated mice was significantly lower than those of untreated control mice. The binding of andrographolide, bisandrographolide A, and bisandrographolide C with CD81 were elucidated by microscale thermophoresis. The suppressive effects of these compounds on the motility of EC109 cells, as well as CD81 protein and mRNA expressions, were further confirmed. CONCLUSION: This is the first time to demonstrate that andrographolide, bisandrographolide A, and bisandrographolide C, which are present in APW, bind to CD81 and suppress its function. These compounds are likely to be responsible for the anti-metastatic activities of APW in esophageal cancer.
Date of acceptance
2022
Autoren
  • Grace Gar-Lee Yue
  • Adele Joyce Gomes
  • Mohamed EM Saeed
  • Kei-Yin Tsui
  • Mona Dawood
  • Assia I Drif
  • Eric Chun-Wai Wong
  • Wai-Fung Lee
  • Wenjing Liu
  • Philip Wai-Yan Chiu
  • Thomas Efferth
  • Clara Bik-San Lau
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/35636176
DOI
10.1016/j.phymed.2022.154183
eISSN
1618-095X
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Andrographis paniculata
  • Bisandrographolide
  • CD81
  • Esophageal cancer
  • Metastasis
  • Tetrasapanin
  • Andrographis paniculata
  • Animals
  • Cell Line, Tumor
  • Diterpenes
  • Down-Regulation
  • Esophageal Neoplasms
  • Humans
  • Mice
  • Molecular Docking Simulation
  • Molecular Targeted Therapy
  • Plant Extracts
  • Tetraspanin 28
Sprache
eng
Country
Germany
Paginierung
154183
PII
S0944-7113(22)00261-6
Datum der Veröffentlichung
2022
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Titel
Identification of active components in Andrographis paniculata targeting on CD81 in esophageal cancer in vitro and in vivo.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
102

Data source: PubMed

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