Antiplanktonic and Antibiofilm Activity of Rheum palmatum against Streptococcus oralis and Porphyromonas gingivalis
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Nadine Kommerein
- Nina Vierengel
- Jonathan Gross
- Till Opatz
- Bilal Al-Nawas
- Lena Katharina Mueller-Heupt
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000802603400001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/microorganisms10050965
- eISSN
- 2076-2607
- Externe Identifier
- Clarivate Analytics Document Solution ID: 1Q3PI
- PubMed Identifier: 35630409
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- MICROORGANISMS
- Schlüsselwörter
- antibiofilm
- biofilm
- antimicrobial
- pathogen-selective therapeutics
- periodontitis
- peri-implantitis
- planktonic
- Porphyromonas gingivalis
- Rheum palmatum
- Streptococcus oralis
- Artikelnummer
- ARTN 965
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Titel
- Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 10
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p>Periodontitis and peri-implantitis are inflammatory conditions with a high global prevalence. Oral pathogens such as Porphyromonas gingivalis play a crucial role in the development of dysbiotic biofilms associated with both diseases. The aim of our study was to identify plant-derived substances which mainly inhibit the growth of “disease promoting bacteria”, by comparing the effect of Rheum palmatum root extract against P. gingivalis and the commensal species Streptococcus oralis. Antiplanktonic activity was determined by measuring optical density and metabolic activity. Antibiofilm activity was quantified using metabolic activity assays and live/dead fluorescence staining combined with confocal laser scanning microscopy. At concentrations of 3.9 mg/L, R. palmatum root extract selectively inhibited planktonic growth of the oral pathogen P. gingivalis, while not inhibiting growth of S. oralis. Selective effects also occurred in mature biofilms, as P. gingivalis was significantly more stressed and inhibited than S. oralis. Our studies show that low concentrations of R. palmatum root extract specifically inhibit P. gingivalis growth, and offer a promising approach for the development of a potential topical agent to prevent alterations in the microbiome due to overgrowth of pathogenic P. gingivalis.</jats:p>
- Autoren
- Nadine Kommerein
- Nina Vierengel
- Jonathan Groß
- Till Opatz
- Bilal Al-Nawas
- Lena Katharina Müller-Heupt
- DOI
- 10.3390/microorganisms10050965
- eISSN
- 2076-2607
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Microorganisms
- Sprache
- en
- Online publication date
- 2022
- Paginierung
- 965 - 965
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/microorganisms10050965
- Datum der Datenerfassung
- 2022
- Titel
- Antiplanktonic and Antibiofilm Activity of Rheum palmatum against Streptococcus oralis and Porphyromonas gingivalis
- Ausgabe der Zeitschrift
- 10
Data source: Crossref
- Abstract
- Periodontitis and peri-implantitis are inflammatory conditions with a high global prevalence. Oral pathogens such as <i>Porphyromonas gingivalis</i> play a crucial role in the development of dysbiotic biofilms associated with both diseases. The aim of our study was to identify plant-derived substances which mainly inhibit the growth of "disease promoting bacteria", by comparing the effect of <i>Rheum palmatum</i> root extract against <i>P. gingivalis</i> and the commensal species <i>Streptococcus oralis</i>. Antiplanktonic activity was determined by measuring optical density and metabolic activity. Antibiofilm activity was quantified using metabolic activity assays and live/dead fluorescence staining combined with confocal laser scanning microscopy. At concentrations of 3.9 mg/L, <i>R. palmatum</i> root extract selectively inhibited planktonic growth of the oral pathogen <i>P. gingivalis</i>, while not inhibiting growth of <i>S. oralis</i>. Selective effects also occurred in mature biofilms, as <i>P. gingivalis</i> was significantly more stressed and inhibited than <i>S. oralis</i>. Our studies show that low concentrations of <i>R. palmatum</i> root extract specifically inhibit <i>P. gingivalis</i> growth, and offer a promising approach for the development of a potential topical agent to prevent alterations in the microbiome due to overgrowth of pathogenic <i>P. gingivalis</i>.
- Addresses
- Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
- Autoren
- Nadine Kommerein
- Nina Vierengel
- Jonathan Groß
- Till Opatz
- Bilal Al-Nawas
- Lena Katharina Müller-Heupt
- DOI
- 10.3390/microorganisms10050965
- eISSN
- 2076-2607
- Externe Identifier
- PubMed Identifier: 35630409
- PubMed Central ID: PMC9143743
- Open access
- true
- ISSN
- 2076-2607
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Microorganisms
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2022
- Open access status
- Open Access
- Paginierung
- 965
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2022
- Titel
- Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i>.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 10
Files
https://www.mdpi.com/2076-2607/10/5/965/pdf?version=1651571586 https://europepmc.org/articles/PMC9143743?pdf=render
Data source: Europe PubMed Central
- Abstract
- Periodontitis and peri-implantitis are inflammatory conditions with a high global prevalence. Oral pathogens such as Porphyromonas gingivalis play a crucial role in the development of dysbiotic biofilms associated with both diseases. The aim of our study was to identify plant-derived substances which mainly inhibit the growth of "disease promoting bacteria", by comparing the effect of Rheum palmatum root extract against P. gingivalis and the commensal species Streptococcus oralis. Antiplanktonic activity was determined by measuring optical density and metabolic activity. Antibiofilm activity was quantified using metabolic activity assays and live/dead fluorescence staining combined with confocal laser scanning microscopy. At concentrations of 3.9 mg/L, R. palmatum root extract selectively inhibited planktonic growth of the oral pathogen P. gingivalis, while not inhibiting growth of S. oralis. Selective effects also occurred in mature biofilms, as P. gingivalis was significantly more stressed and inhibited than S. oralis. Our studies show that low concentrations of R. palmatum root extract specifically inhibit P. gingivalis growth, and offer a promising approach for the development of a potential topical agent to prevent alterations in the microbiome due to overgrowth of pathogenic P. gingivalis.
- Date of acceptance
- 2022
- Autoren
- Nadine Kommerein
- Nina Vierengel
- Jonathan Groß
- Till Opatz
- Bilal Al-Nawas
- Lena Katharina Müller-Heupt
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/35630409
- DOI
- 10.3390/microorganisms10050965
- Externe Identifier
- PubMed Central ID: PMC9143743
- ISSN
- 2076-2607
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Microorganisms
- Schlüsselwörter
- Porphyromonas gingivalis
- Rheum palmatum
- Streptococcus oralis
- antibiofilm
- antimicrobial
- biofilm
- pathogen-selective therapeutics
- peri-implantitis
- periodontitis
- planktonic
- Sprache
- eng
- Country
- Switzerland
- PII
- microorganisms10050965
- Datum der Veröffentlichung
- 2022
- Status
- Published online
- Titel
- Antiplanktonic and Antibiofilm Activity of Rheum palmatum against Streptococcus oralis and Porphyromonas gingivalis.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 10
Data source: PubMed
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- Property of