Fishing for artemisin-ininteracting proteins from human nasopharyngeal cancer cells

Publication type:
Conference
Metadata:
Autoren
  • Tolga Eichhorn
  • Siegfried Schloissnig
  • Wolf-Dieter Lehmann
  • Rolf Mertens
  • Luise Krauth-Siegel
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000209701600133&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1158/1538-7445.AM2012-1993
eISSN
1538-7445
Externe Identifier
  • Clarivate Analytics Document Solution ID: V43SR
ISSN
0008-5472
Zeitschrift
CANCER RESEARCH
Datum der Veröffentlichung
2012
Status
Published
Titel
Fishing for artemisin-ininteracting proteins from human nasopharyngeal cancer cells
Ausgabe der Zeitschrift
72

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title> <jats:p>Determining cellular target molecules of drugs by chemical proteomic techniques is complex and tedious. Most approaches rely on activity-based probe profiling and compound-centric chemical proteomics. The antimalarial artemisinin also exerts profound anti-cancer activity, but the mechanisms of action are incompletely understood. In the present study, we have identified artemisinin-interacting target proteins from human nasopharyngeal carcinoma cell line CNE1. Thereby, our approach overcomes usual problems in traditional fishing procedures, because the drug was attached to a polystyrene surface without further chemical modification. Using mass spectrometry we have identified 20 proteins which effect cell cycle arrest, apoptosis, inhibition of angiogenesis, disruption of cell migration, and modulation of nuclear receptor responsiveness. One protein out of the twenty was further investigated in vitro for direct ligand binding. Furthermore, a blind-docking based approach confirmed experimentally identified proteins and suggested further interacting proteins. Theoretically predicted interaction partners may serve as a starting point to complete the whole set of proteins binding artemisinin. In our investigations, we have comprised artemisinins role in cancer treatment in a greater shape through a systematic biological strategy and thus, are able to show novel insights into its mode of action.</jats:p> <jats:p>Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1993. doi:1538-7445.AM2012-1993</jats:p>
Autoren
  • Tolga Eichhorn
  • Siegfried Schloissnig
  • Wolf-Dieter Lehmann
  • Rolf Mertens
  • Luise Krauth-Siegel
  • Thomas Efferth
DOI
10.1158/1538-7445.am2012-1993
eISSN
1538-7445
ISSN
0008-5472
Ausgabe der Veröffentlichung
8_Supplement
Zeitschrift
Cancer Research
Paginierung
1993 - 1993
Datum der Veröffentlichung
2012
Status
Published
Herausgeber
American Association for Cancer Research (AACR)
Herausgeber URL
http://dx.doi.org/10.1158/1538-7445.am2012-1993
Datum der Datenerfassung
2022
Titel
Abstract 1993: Fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells
Ausgabe der Zeitschrift
72

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