RAPID DETECTION ASSAYS FOR MULTIDRUG RESISTANCE
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- T EFFERTH
- M VOLM
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:A1988R460900003&DestLinkType=FullRecord&DestApp=WOS_CPL
- Externe Identifier
- Clarivate Analytics Document Solution ID: R4609
- PubMed Identifier: 3245849
- ISSN
- 0004-4172
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- ARZNEIMITTEL-FORSCHUNG/DRUG RESEARCH
- Paginierung
- 1771 - 1774
- Datum der Veröffentlichung
- 1988
- Status
- Published
- Titel
- RAPID DETECTION ASSAYS FOR MULTIDRUG RESISTANCE
- Sub types
- Article
- Ausgabe der Zeitschrift
- 38-2
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- In attempts to develop simple and rapid assays for detection of multidrug resistance (MDR) doxorubicin-resistant S180 (S180DOX), colchicine-resistant CHO (CHOCOL) and cytarabine (cytosine-arabinoside)-resistant L1210 cell lines (L1210AraC) were investigated. S180DOX and CHOCOL cells express the MDR-phenotype while L1210AraC does not. Using a previously described short-term assay firstly, inhibition of incorporation of radioactive nucleic acid precursors into tumour cells after addition of doxorubicin was measured. Secondly, the accumulation of the fluorescent dye rhodamine 123 (R123) in the different cell lines were analyzed. It could be observed that the resistant S180DOX and CHOCOL cells needed significantly more time to accumulate R123 than their sensitive parental cell lines or L1210AraC cells. Therefore, both assays are adequate tools for the rapid detection of MDR.
- Addresses
- Institute of Experimental Pathology, German Cancer Research Center, Heidelberg.
- Autoren
- T Efferth
- M Volm
- eISSN
- 1616-7066
- Externe Identifier
- PubMed Identifier: 3245849
- Open access
- false
- ISSN
- 0004-4172
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- Arzneimittel-Forschung
- Schlüsselwörter
- Cell Line
- Animals
- Cricetulus
- Leukemia L1210
- Sarcoma 180
- Colchicine
- Vincristine
- Dactinomycin
- Rhodamines
- Rhodamine 123
- Doxorubicin
- Cytarabine
- Antineoplastic Combined Chemotherapy Protocols
- Drug Resistance
- Female
- Cricetinae
- Sprache
- eng
- Medium
- Paginierung
- 1771 - 1774
- Datum der Veröffentlichung
- 1988
- Status
- Published
- Datum der Datenerfassung
- 1988
- Titel
- Rapid detection assays for multidrug resistance.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 38
Data source: Europe PubMed Central
- Abstract
- In attempts to develop simple and rapid assays for detection of multidrug resistance (MDR) doxorubicin-resistant S180 (S180DOX), colchicine-resistant CHO (CHOCOL) and cytarabine (cytosine-arabinoside)-resistant L1210 cell lines (L1210AraC) were investigated. S180DOX and CHOCOL cells express the MDR-phenotype while L1210AraC does not. Using a previously described short-term assay firstly, inhibition of incorporation of radioactive nucleic acid precursors into tumour cells after addition of doxorubicin was measured. Secondly, the accumulation of the fluorescent dye rhodamine 123 (R123) in the different cell lines were analyzed. It could be observed that the resistant S180DOX and CHOCOL cells needed significantly more time to accumulate R123 than their sensitive parental cell lines or L1210AraC cells. Therefore, both assays are adequate tools for the rapid detection of MDR.
- Autoren
- T Efferth
- M Volm
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/3245849
- ISSN
- 0004-4172
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- Arzneimittelforschung
- Schlüsselwörter
- Animals
- Antineoplastic Combined Chemotherapy Protocols
- Cell Line
- Colchicine
- Cricetinae
- Cricetulus
- Cytarabine
- Dactinomycin
- Doxorubicin
- Drug Resistance
- Female
- Leukemia L1210
- Rhodamine 123
- Rhodamines
- Sarcoma 180
- Vincristine
- Sprache
- eng
- Country
- Germany
- Paginierung
- 1771 - 1774
- Datum der Veröffentlichung
- 1988
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 1989
- Titel
- Rapid detection assays for multidrug resistance.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 38
Data source: PubMed
- Beziehungen:
- Property of