INDUCED MULTIDRUG-RESISTANCE IN MURINE SARCOMA 180 CELLS GROWN-INVITRO AND INVIVO AND ASSOCIATED CHANGES IN EXPRESSION OF MULTIDRUG-RESISTANCE DNA-SEQUENCES AND MEMBRANE-GLYCOPROTEINS

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:A1988T038000003&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: T0380
  • PubMed Identifier: 3218953
ISSN
0250-7005
Ausgabe der Veröffentlichung
6
Zeitschrift
ANTICANCER RESEARCH
Paginierung
1169 - 1178
Datum der Veröffentlichung
1988
Status
Published
Titel
INDUCED MULTIDRUG-RESISTANCE IN MURINE SARCOMA 180 CELLS GROWN-INVITRO AND INVIVO AND ASSOCIATED CHANGES IN EXPRESSION OF MULTIDRUG-RESISTANCE DNA-SEQUENCES AND MEMBRANE-GLYCOPROTEINS
Sub types
  • Article
Ausgabe der Zeitschrift
8

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
The aim of this investigation was to analyze the resistance to doxorubicin and daunorubicin of murine sarcoma 180 cells grown in vitro (monolayer) and in vivo (ascites form). The colchicine-resistant CHO cells were used as controls. A multidrug-resistant phenotype was found in all investigated cell lines. Multidrug resistant cells grown in tissue culture or as ascites tumor cells needed more time to accumulate rhodamine 123 than their sensitive parental cells. In order to evaluate whether the resistant cells show alterations in the plasma membranes, different methods were applied (immunocytochemistry, immunofluorescence, immunoblotting) using the monoclonal antibodies 265/F4 and C 219. These methods all revealed an increased expression of the glycoprotein Mr 170 kd in the multidrug-resistant cell lines. To determine whether multidrug DNA sequences were expressed in the resistant cell lines, slot blots and Northern blots with RNA of sensitive and resistant cells were performed using the clones pDR 7.8 and pcDR 1.5. Elevated RNA levels were detected in all resistant cell lines.
Addresses
  • German Cancer Research Center, Institute of Experimental Pathology, Heidelberg.
Autoren
eISSN
1791-7530
Externe Identifier
  • PubMed Identifier: 3218953
Open access
false
ISSN
0250-7005
Ausgabe der Veröffentlichung
6
Zeitschrift
Anticancer research
Schlüsselwörter
  • Cell Line
  • Animals
  • Mice
  • Sarcoma 180
  • Colchicine
  • Dactinomycin
  • Daunorubicin
  • Doxorubicin
  • Membrane Glycoproteins
  • DNA, Neoplasm
  • Cytarabine
  • Cell Division
  • Drug Resistance
Sprache
eng
Medium
Print
Paginierung
1169 - 1178
Datum der Veröffentlichung
1988
Status
Published
Datum der Datenerfassung
1988
Titel
Induced multidrug-resistance in murine sarcoma 180 cells grown in vitro and in vivo and associated changes in expression of multidrug-resistance DNA-sequences and membrane glycoproteins.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
8

Data source: Europe PubMed Central

Abstract
The aim of this investigation was to analyze the resistance to doxorubicin and daunorubicin of murine sarcoma 180 cells grown in vitro (monolayer) and in vivo (ascites form). The colchicine-resistant CHO cells were used as controls. A multidrug-resistant phenotype was found in all investigated cell lines. Multidrug resistant cells grown in tissue culture or as ascites tumor cells needed more time to accumulate rhodamine 123 than their sensitive parental cells. In order to evaluate whether the resistant cells show alterations in the plasma membranes, different methods were applied (immunocytochemistry, immunofluorescence, immunoblotting) using the monoclonal antibodies 265/F4 and C 219. These methods all revealed an increased expression of the glycoprotein Mr 170 kd in the multidrug-resistant cell lines. To determine whether multidrug DNA sequences were expressed in the resistant cell lines, slot blots and Northern blots with RNA of sensitive and resistant cells were performed using the clones pDR 7.8 and pcDR 1.5. Elevated RNA levels were detected in all resistant cell lines.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/3218953
ISSN
0250-7005
Ausgabe der Veröffentlichung
6
Zeitschrift
Anticancer Res
Schlüsselwörter
  • Animals
  • Cell Division
  • Cell Line
  • Colchicine
  • Cytarabine
  • DNA, Neoplasm
  • Dactinomycin
  • Daunorubicin
  • Doxorubicin
  • Drug Resistance
  • Membrane Glycoproteins
  • Mice
  • Sarcoma 180
Sprache
eng
Country
Greece
Paginierung
1169 - 1178
Datum der Veröffentlichung
1988
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
1989
Titel
Induced multidrug-resistance in murine sarcoma 180 cells grown in vitro and in vivo and associated changes in expression of multidrug-resistance DNA-sequences and membrane glycoproteins.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
8

Data source: PubMed

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