OVEREXPRESSION OF P-GLYCOPROTEIN IN RAT HEPATOCELLULAR CARCINOMAS INDUCED WITH N-NITROSOMORPHOLINE

Publication type:
Journal article
Metadata:
Autoren
  • M VOLM
  • H ZERBAN
  • J MATTERN
  • T EFFERTH
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:A1990CJ85000027&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1093/carcin/11.1.169
Externe Identifier
  • Clarivate Analytics Document Solution ID: CJ850
  • PubMed Identifier: 1967229
ISSN
0143-3334
Ausgabe der Veröffentlichung
1
Zeitschrift
CARCINOGENESIS
Paginierung
169 - 172
Datum der Veröffentlichung
1990
Status
Published
Titel
OVEREXPRESSION OF P-GLYCOPROTEIN IN RAT HEPATOCELLULAR CARCINOMAS INDUCED WITH N-NITROSOMORPHOLINE
Sub types
  • Note
Ausgabe der Zeitschrift
11

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • M Volm
  • H Zerban
  • J Mattern
  • Th Efferth
DOI
10.1093/carcin/11.1.169
eISSN
1460-2180
ISSN
0143-3334
Ausgabe der Veröffentlichung
1
Zeitschrift
Carcinogenesis
Sprache
en
Paginierung
169 - 172
Datum der Veröffentlichung
1990
Status
Published
Herausgeber
Oxford University Press (OUP)
Herausgeber URL
http://dx.doi.org/10.1093/carcin/11.1.169
Datum der Datenerfassung
2017
Titel
Overexpression of P-glycoprotein rat hepatocellular carcinomas induced with <i>N</i>-nitrosomorpholine
Ausgabe der Zeitschrift
11

Data source: Crossref

Abstract
The most frequently reported alteration of multidrug resistance (MDR) is the overexpression of the 170 kd membrane glycoprotein. An increased expression of the MDR-gene in pre-neoplastic and neoplastic liver lesions produced experimentally by different carcinogens has been reported. As shown in rat liver by stop experiments, specific carcinogen-induced alterations can be separated from non-specific, toxic changes. Therefore, we induced rat hepatocellular carcinomas with N-nitrosomorpholine and investigated whether expression of the MDR-gene also takes place in hepatocellular carcinomas after withdrawal of the carcinogen. Using mAb C219 against P-glycoprotein, both normal liver and hepatocellular carcinomas show specific immunoreactivity by means of immunofluorescence and immunohistochemistry. In hepatocellular carcinomas, however, the reaction is increased if compared to normal liver of untreated control animals. These results were confirmed by immunoblotting. Using the cDNA probe 1.5, a significant increase in MDR-gene transcripts was found in hepatocellular carcinomas as compared to normal liver.
Addresses
  • Department of Experimental Pathology, German Cancer Research Center, Heidelberg, FRG.
Autoren
  • M Volm
  • H Zerban
  • J Mattern
  • T Efferth
  • T Efferth
DOI
10.1093/carcin/11.1.169
eISSN
1460-2180
Externe Identifier
  • PubMed Identifier: 1967229
Open access
false
ISSN
0143-3334
Ausgabe der Veröffentlichung
1
Zeitschrift
Carcinogenesis
Schlüsselwörter
  • Liver
  • Animals
  • Rats, Inbred Lew
  • Rats
  • Liver Neoplasms, Experimental
  • Nitrosamines
  • Actins
  • Membrane Glycoproteins
  • Neoplasm Proteins
  • Antibodies, Monoclonal
  • Carcinogens
  • Fluorescent Antibody Technique
  • Immunohistochemistry
  • Gene Expression
  • Transcription, Genetic
  • Drug Resistance
  • Reference Values
  • Male
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
Sprache
eng
Medium
Print
Paginierung
169 - 172
Datum der Veröffentlichung
1990
Status
Published
Datum der Datenerfassung
1990
Titel
Overexpression of P-glycoprotein in rat hepatocellular carcinomas induced with N-nitrosomorpholine.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
11

Data source: Europe PubMed Central

Abstract
The most frequently reported alteration of multidrug resistance (MDR) is the overexpression of the 170 kd membrane glycoprotein. An increased expression of the MDR-gene in pre-neoplastic and neoplastic liver lesions produced experimentally by different carcinogens has been reported. As shown in rat liver by stop experiments, specific carcinogen-induced alterations can be separated from non-specific, toxic changes. Therefore, we induced rat hepatocellular carcinomas with N-nitrosomorpholine and investigated whether expression of the MDR-gene also takes place in hepatocellular carcinomas after withdrawal of the carcinogen. Using mAb C219 against P-glycoprotein, both normal liver and hepatocellular carcinomas show specific immunoreactivity by means of immunofluorescence and immunohistochemistry. In hepatocellular carcinomas, however, the reaction is increased if compared to normal liver of untreated control animals. These results were confirmed by immunoblotting. Using the cDNA probe 1.5, a significant increase in MDR-gene transcripts was found in hepatocellular carcinomas as compared to normal liver.
Autoren
  • M Volm
  • H Zerban
  • J Mattern
  • T Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/1967229
DOI
10.1093/carcin/11.1.169
ISSN
0143-3334
Ausgabe der Veröffentlichung
1
Zeitschrift
Carcinogenesis
Schlüsselwörter
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Actins
  • Animals
  • Antibodies, Monoclonal
  • Carcinogens
  • Drug Resistance
  • Fluorescent Antibody Technique
  • Gene Expression
  • Immunohistochemistry
  • Liver
  • Liver Neoplasms, Experimental
  • Male
  • Membrane Glycoproteins
  • Neoplasm Proteins
  • Nitrosamines
  • Rats
  • Rats, Inbred Lew
  • Reference Values
  • Transcription, Genetic
Sprache
eng
Country
England
Paginierung
169 - 172
Datum der Veröffentlichung
1990
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
1990
Titel
Overexpression of P-glycoprotein in rat hepatocellular carcinomas induced with N-nitrosomorpholine.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
11

Data source: PubMed

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