EXPRESSION OF APOPTOSIS-RELATED ONCOPROTEINS AND MODULATION OF APOPTOSIS BY CAFFEINE IN HUMAN LEUKEMIC-CELLS

Publication type:
Journal article
Metadata:
Autoren
  • T EFFERTH
  • U FABRY
  • P GLATTE
  • R OSIEKA
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:A1995TE36000004&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1007/BF01218522
Externe Identifier
  • Clarivate Analytics Document Solution ID: TE360
  • PubMed Identifier: 7593128
ISSN
0171-5216
Ausgabe der Veröffentlichung
11
Zeitschrift
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Schlüsselwörter
  • RADIO- AND CHEMOSENSITIVITY
  • FLOW CYTOMETRY
  • IMMUNOCYTOCHEMISTRY
  • MTT ASSAY
  • MULTIVARIATE CLUSTER ANALYSIS
Paginierung
648 - 656
Datum der Veröffentlichung
1995
Status
Published
Titel
EXPRESSION OF APOPTOSIS-RELATED ONCOPROTEINS AND MODULATION OF APOPTOSIS BY CAFFEINE IN HUMAN LEUKEMIC-CELLS
Sub types
  • Article
Ausgabe der Zeitschrift
121

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • T Efferth
  • U Fabry
  • P Glatte
  • R Osieka
DOI
10.1007/bf01218522
eISSN
1432-1335
ISSN
0171-5216
Ausgabe der Veröffentlichung
11
Zeitschrift
Journal of Cancer Research and Clinical Oncology
Sprache
en
Paginierung
648 - 656
Datum der Veröffentlichung
1995
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1007/bf01218522
Datum der Datenerfassung
2021
Titel
Expression of apoptosis-related oncoproteins and modulation of apoptosis by caffeine in human leukemic cells
Ausgabe der Zeitschrift
121

Data source: Crossref

Abstract
We investigated the modulation of radio- and chemoresistance by caffeine and mechanisms of resistance in human leukemic cell lines and mononuclear cells from 18 leukemic patients. Caffeine synergistically potentiated cytotoxicity and apoptosis induced by ionizing radiation or carboplatin (CPt), but attenuated induction of apoptosis by daunorubicin (DNR) in KG-1a cells. Since caffeine released irradiated as well as DNR-treated KG-1a cells from G2M cell cycle arrest and CPt-treated cells from S-phase arrest, this release does not fully explain the different effects of caffeine. Caffeine synergistically reduced the level of the apoptosis inhibitor glutathione after irradiation or CPt treatment. In contrast, treatment with DNR plus caffeine diminished glutathione levels to a lesser extent than DNR alone. We conclude that the effect of caffeine on glutathione depletion represents a mechanism of action by which caffeine can modulate apoptosis. Caffeine increased CPt cytotoxicity in K562 cells and its doxorubicin-resistant subline (K562/ADM), but little effect was seen in HL-60 cells or mononuclear cells from leukemic patients. Multivariate cluster analysis revealed an association of CPt resistance with the expression of c-Fos, c-N-Ras, and p53 oncoproteins and with proliferative activity (S-phase of cell cycle), but not with Bcl-2 expression.
Addresses
  • Medizinische Klinik IV, RWTH Aachen, Germany.
Autoren
  • T Efferth
  • T Efferth
  • U Fabry
  • P Glatte
  • R Osieka
DOI
10.1007/bf01218522
eISSN
1432-1335
Externe Identifier
  • PubMed Identifier: 7593128
Open access
false
ISSN
0171-5216
Ausgabe der Veröffentlichung
11
Zeitschrift
Journal of cancer research and clinical oncology
Schlüsselwörter
  • HL-60 Cells
  • Tumor Cells, Cultured
  • Humans
  • Leukemia, Myeloid
  • Leukemia, Promyelocytic, Acute
  • Lymphoma, Non-Hodgkin
  • Carboplatin
  • Tetrazolium Salts
  • Thiazoles
  • Caffeine
  • Daunorubicin
  • GTP-Binding Proteins
  • ras Proteins
  • Glutathione
  • Oncogene Proteins v-fos
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Flow Cytometry
  • Immunohistochemistry
  • Multivariate Analysis
  • Cluster Analysis
  • G2 Phase
  • S Phase
  • Apoptosis
  • Gene Expression Regulation, Leukemic
  • Drug Resistance, Neoplasm
  • Gamma Rays
  • Radiation Tolerance
  • Coloring Agents
  • Tumor Suppressor Protein p53
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive
  • Leukemia, Myeloid, Acute
Sprache
eng
Medium
Print
Paginierung
648 - 656
Datum der Veröffentlichung
1995
Status
Published
Datum der Datenerfassung
1995
Titel
Expression of apoptosis-related oncoproteins and modulation of apoptosis by caffeine in human leukemic cells.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
121

Data source: Europe PubMed Central

Abstract
We investigated the modulation of radio- and chemoresistance by caffeine and mechanisms of resistance in human leukemic cell lines and mononuclear cells from 18 leukemic patients. Caffeine synergistically potentiated cytotoxicity and apoptosis induced by ionizing radiation or carboplatin (CPt), but attenuated induction of apoptosis by daunorubicin (DNR) in KG-1a cells. Since caffeine released irradiated as well as DNR-treated KG-1a cells from G2M cell cycle arrest and CPt-treated cells from S-phase arrest, this release does not fully explain the different effects of caffeine. Caffeine synergistically reduced the level of the apoptosis inhibitor glutathione after irradiation or CPt treatment. In contrast, treatment with DNR plus caffeine diminished glutathione levels to a lesser extent than DNR alone. We conclude that the effect of caffeine on glutathione depletion represents a mechanism of action by which caffeine can modulate apoptosis. Caffeine increased CPt cytotoxicity in K562 cells and its doxorubicin-resistant subline (K562/ADM), but little effect was seen in HL-60 cells or mononuclear cells from leukemic patients. Multivariate cluster analysis revealed an association of CPt resistance with the expression of c-Fos, c-N-Ras, and p53 oncoproteins and with proliferative activity (S-phase of cell cycle), but not with Bcl-2 expression.
Autoren
  • T Efferth
  • U Fabry
  • P Glatte
  • R Osieka
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/7593128
DOI
10.1007/BF01218522
ISSN
0171-5216
Ausgabe der Veröffentlichung
11
Zeitschrift
J Cancer Res Clin Oncol
Schlüsselwörter
  • Apoptosis
  • Caffeine
  • Carboplatin
  • Cluster Analysis
  • Coloring Agents
  • Daunorubicin
  • Drug Resistance, Neoplasm
  • Flow Cytometry
  • G2 Phase
  • GTP-Binding Proteins
  • Gamma Rays
  • Gene Expression Regulation, Leukemic
  • Glutathione
  • HL-60 Cells
  • Humans
  • Immunohistochemistry
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive
  • Leukemia, Myeloid
  • Leukemia, Myeloid, Acute
  • Leukemia, Promyelocytic, Acute
  • Lymphoma, Non-Hodgkin
  • Multivariate Analysis
  • Oncogene Proteins v-fos
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Radiation Tolerance
  • S Phase
  • Tetrazolium Salts
  • Thiazoles
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53
  • ras Proteins
Sprache
eng
Country
Germany
Paginierung
648 - 656
Datum der Veröffentlichung
1995
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
1995
Titel
Expression of apoptosis-related oncoproteins and modulation of apoptosis by caffeine in human leukemic cells.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
121

Data source: PubMed

Beziehungen:
Property of

Tools