SISTER-CHROMATID EXCHANGE INDUCING DNA LESIONS AND DEPRESSION OF ACTIVATION MARKERS ON THE SURFACE OF CULTURED PERIPHERAL-BLOOD MONONUCLEAR-CELLS AFTER THE ADDITION OF STREPTOCOCCAL PYROGENIC EXOTOXIN-A AND EXOTOXINC

Publication type:
Journal article
Metadata:
Autoren
  • A BUSSING
  • M KLOTZ
  • K SUZART
  • T EFFERTH
  • D GERLACH
  • N SCHNITZLER
  • R OSIEKA
  • K SCHWEIZER
  • A KAUFHOLD
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:A1995RT24000006&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: RT240
  • PubMed Identifier: 7500916
ISSN
0300-8584
Ausgabe der Veröffentlichung
2
Zeitschrift
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
Paginierung
87 - 96
Datum der Veröffentlichung
1995
Status
Published
Titel
SISTER-CHROMATID EXCHANGE INDUCING DNA LESIONS AND DEPRESSION OF ACTIVATION MARKERS ON THE SURFACE OF CULTURED PERIPHERAL-BLOOD MONONUCLEAR-CELLS AFTER THE ADDITION OF STREPTOCOCCAL PYROGENIC EXOTOXIN-A AND EXOTOXINC
Sub types
  • Article
Ausgabe der Zeitschrift
184

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Arndt B�ssing
  • Martina Klotz
  • Kiliana Suzart
  • Thomas Efferth
  • Dieter Gerlach
  • Norbert Schnitzler
  • Reinhardt Osieka
  • Klaus Schweizer
  • Achim Kaufhold
DOI
10.1007/bf00221392
eISSN
1432-1831
ISSN
0300-8584
Ausgabe der Veröffentlichung
2
Zeitschrift
Medical Microbiology and Immunology
Sprache
en
Datum der Veröffentlichung
1995
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1007/bf00221392
Datum der Datenerfassung
2019
Titel
Sister chromatid exchange-inducing DNA lesions and depression of activation markers on the surface of cultured peripheral blood mononuclear cells after the addition of streptococcal pyrogenic exotoxins A and C
Ausgabe der Zeitschrift
184

Data source: Crossref

Abstract
Cultivation of peripheral blood mononuclear cells (PBMC) in the presence of streptococcal pyrogenic exotoxins (SPE) A and C resulted in a significant induction of sister chromatid exchange (SCE)-inducing DNA lesions. Concomitantly, the expression of interleukin-2 receptor alpha chain (IL-2R alpha chain), transferrin receptor (TfR), and major histocompatibility complex class II molecule HLA-DR on the surface of phytohemagglutinin-activated T cells from whole blood culture cells (WBCC) significantly decreased within 72 h, that is at least two cell cycles, whereas unstimulated T cells from WBCC did not express these markers but had lost their CD3 molecules, an effect reported to precede apoptosis as part of a T cell inactivation pathway. However, no apoptotic cells were observed within a cultivation period of 120 h. We observed clearcut differences in the responses towards SPE A in WBCC and isolated lymphocytes, since SPE A-treated lymphocytes showed an increase in the [3H]thymidine incorporation and did express IL-2R alpha chain and TfR on their cell surface. Regardless of the precise underlying mechanism, T cells from WBCC seem to be in a state of functional incompetence. The data presented here are the first to provide strong evidence that streptococcal toxins produce SCE-inducing DNA lesions in PBMC, an effect that might contribute to the process of immune cell lethality in streptococcal toxic shock-like syndrome and could be of pivotal importance in the pathogenesis of severe streptococcal disease.
Addresses
  • Institute of Medical Immunology, Technical University (RWTH) Aachen, Germany.
Autoren
  • A Büssing
  • M Klotz
  • K Suzart
  • T Efferth
  • T Efferth
  • D Gerlach
  • N Schnitzler
  • R Osieka
  • K Schweizer
  • A Kaufhold
DOI
10.1007/bf00221392
eISSN
1432-1831
Externe Identifier
  • PubMed Identifier: 7500916
Open access
false
ISSN
0300-8584
Ausgabe der Veröffentlichung
2
Zeitschrift
Medical microbiology and immunology
Schlüsselwörter
  • Leukocytes, Mononuclear
  • Cells, Cultured
  • Humans
  • Streptococcus pyogenes
  • DNA Damage
  • Bacterial Proteins
  • Receptors, Transferrin
  • Membrane Proteins
  • Antigens, CD
  • Interleukin-2
  • Exotoxins
  • HLA-DR Antigens
  • Flow Cytometry
  • Cell Cycle
  • Cell Division
  • Sister Chromatid Exchange
  • Adult
Sprache
eng
Medium
Print
Paginierung
87 - 96
Datum der Veröffentlichung
1995
Status
Published
Datum der Datenerfassung
1995
Titel
Sister chromatid exchange-inducing DNA lesions and depression of activation markers on the surface of cultured peripheral blood mononuclear cells after the addition of streptococcal pyrogenic exotoxins A and C.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
184

Data source: Europe PubMed Central

Abstract
Cultivation of peripheral blood mononuclear cells (PBMC) in the presence of streptococcal pyrogenic exotoxins (SPE) A and C resulted in a significant induction of sister chromatid exchange (SCE)-inducing DNA lesions. Concomitantly, the expression of interleukin-2 receptor alpha chain (IL-2R alpha chain), transferrin receptor (TfR), and major histocompatibility complex class II molecule HLA-DR on the surface of phytohemagglutinin-activated T cells from whole blood culture cells (WBCC) significantly decreased within 72 h, that is at least two cell cycles, whereas unstimulated T cells from WBCC did not express these markers but had lost their CD3 molecules, an effect reported to precede apoptosis as part of a T cell inactivation pathway. However, no apoptotic cells were observed within a cultivation period of 120 h. We observed clearcut differences in the responses towards SPE A in WBCC and isolated lymphocytes, since SPE A-treated lymphocytes showed an increase in the [3H]thymidine incorporation and did express IL-2R alpha chain and TfR on their cell surface. Regardless of the precise underlying mechanism, T cells from WBCC seem to be in a state of functional incompetence. The data presented here are the first to provide strong evidence that streptococcal toxins produce SCE-inducing DNA lesions in PBMC, an effect that might contribute to the process of immune cell lethality in streptococcal toxic shock-like syndrome and could be of pivotal importance in the pathogenesis of severe streptococcal disease.
Autoren
  • A Büssing
  • M Klotz
  • K Suzart
  • T Efferth
  • D Gerlach
  • N Schnitzler
  • R Osieka
  • K Schweizer
  • A Kaufhold
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/7500916
DOI
10.1007/BF00221392
ISSN
0300-8584
Ausgabe der Veröffentlichung
2
Zeitschrift
Med Microbiol Immunol
Schlüsselwörter
  • Adult
  • Antigens, CD
  • Bacterial Proteins
  • Cell Cycle
  • Cell Division
  • Cells, Cultured
  • DNA Damage
  • Exotoxins
  • Flow Cytometry
  • HLA-DR Antigens
  • Humans
  • Interleukin-2
  • Leukocytes, Mononuclear
  • Membrane Proteins
  • Receptors, Transferrin
  • Sister Chromatid Exchange
  • Streptococcus pyogenes
Sprache
eng
Country
Germany
Paginierung
87 - 96
Datum der Veröffentlichung
1995
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
1996
Titel
Sister chromatid exchange-inducing DNA lesions and depression of activation markers on the surface of cultured peripheral blood mononuclear cells after the addition of streptococcal pyrogenic exotoxins A and C.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
184

Data source: PubMed

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