Leptin contributes to the protection of human leukemic cells from cisplatinum cytotoxicity

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000088559700043&DestLinkType=FullRecord&DestApp=WOS_CPL
eISSN
1791-7530
Externe Identifier
  • Clarivate Analytics Document Solution ID: 340WT
  • PubMed Identifier: 10953324
ISSN
0250-7005
Ausgabe der Veröffentlichung
4
Zeitschrift
ANTICANCER RESEARCH
Schlüsselwörter
  • antisense phosphorothioate oligodeoxynucleotides
  • peptide nucleic acids
  • real time TaqMan (R) polymerase chain reaction
Paginierung
2541 - 2546
Datum der Veröffentlichung
2000
Status
Published
Titel
Leptin contributes to the protection of human leukemic cells from cisplatinum cytotoxicity
Sub types
  • Article
Ausgabe der Zeitschrift
20

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
Leptin (ob gene) and its cognate receptor (obr) are relevant for fat metabolism. Obr shares homology with the IL-6 signal transducer gp130 and is expressed in hematopoietic cells. Since cytokines and growth factors regulate both hematopoiesis and response to chemotherapy, we tested the hypothesis of whether leptin protects leukemic cells from cytotoxicity of cisplatinum. Antisense phosphorothioate oligodeoxynucleotides (ODNs) and antisense peptide nucleic acids (PNAs) complementary to the obr gene were first tested for their growth inhibitory activity in obr expressing leukemic cells. Liposome-mediated transfection of ODNs (1-2 microM) or PNAs (0.01-1 microM) inhibited growth up to 50%. Combination treatments of cisplatinum and 0.01 microM PNA reduced growth more than cisplatinum alone. Vice versa, recombinant human leptin (rhL) diminished cisplatinum-induced growth inhibition. Finally, we investigated whether rhL affects cisplatinum-induced DNA damage and repair in the housekeeping gene beta-actin by means of real time TaqMan polymerase chain reaction. RhL reduced DNA damage and increased DNA repair. The effects are, however, modest and leptin is probably not the only player in the armory of growth factors which affect drug resistance.
Addresses
  • Hospital for Internal Medicine IV, RWTH Aachen, Germany. tseffert@viro.med.uni-erlangen.de
Autoren
eISSN
1791-7530
Externe Identifier
  • PubMed Identifier: 10953324
Open access
false
ISSN
0250-7005
Ausgabe der Veröffentlichung
4
Zeitschrift
Anticancer research
Schlüsselwörter
  • K562 Cells
  • Humans
  • DNA Damage
  • Cisplatin
  • Leptin
  • DNA-Binding Proteins
  • Trans-Activators
  • Oligonucleotides, Antisense
  • Antineoplastic Agents
  • Polymerase Chain Reaction
  • Cell Survival
  • DNA Repair
  • Dose-Response Relationship, Drug
  • STAT3 Transcription Factor
Sprache
eng
Medium
Print
Paginierung
2541 - 2546
Datum der Veröffentlichung
2000
Status
Published
Datum der Datenerfassung
2000
Titel
Leptin contributes to the protection of human leukemic cells from cisplatinum cytoxicity.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
20

Data source: Europe PubMed Central

Abstract
Leptin (ob gene) and its cognate receptor (obr) are relevant for fat metabolism. Obr shares homology with the IL-6 signal transducer gp130 and is expressed in hematopoietic cells. Since cytokines and growth factors regulate both hematopoiesis and response to chemotherapy, we tested the hypothesis of whether leptin protects leukemic cells from cytotoxicity of cisplatinum. Antisense phosphorothioate oligodeoxynucleotides (ODNs) and antisense peptide nucleic acids (PNAs) complementary to the obr gene were first tested for their growth inhibitory activity in obr expressing leukemic cells. Liposome-mediated transfection of ODNs (1-2 microM) or PNAs (0.01-1 microM) inhibited growth up to 50%. Combination treatments of cisplatinum and 0.01 microM PNA reduced growth more than cisplatinum alone. Vice versa, recombinant human leptin (rhL) diminished cisplatinum-induced growth inhibition. Finally, we investigated whether rhL affects cisplatinum-induced DNA damage and repair in the housekeeping gene beta-actin by means of real time TaqMan polymerase chain reaction. RhL reduced DNA damage and increased DNA repair. The effects are, however, modest and leptin is probably not the only player in the armory of growth factors which affect drug resistance.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/10953324
ISSN
0250-7005
Ausgabe der Veröffentlichung
4
Zeitschrift
Anticancer Res
Schlüsselwörter
  • Antineoplastic Agents
  • Cell Survival
  • Cisplatin
  • DNA Damage
  • DNA Repair
  • DNA-Binding Proteins
  • Dose-Response Relationship, Drug
  • Humans
  • K562 Cells
  • Leptin
  • Oligonucleotides, Antisense
  • Polymerase Chain Reaction
  • STAT3 Transcription Factor
  • Trans-Activators
Sprache
eng
Country
Greece
Paginierung
2541 - 2546
Datum der Veröffentlichung
2000
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2000
Titel
Leptin contributes to the protection of human leukemic cells from cisplatinum cytoxicity.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
20

Data source: PubMed

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