Differential expression of the multidrug resistance-related protein MRP1 in the histological compartments of nephroblastomas

Autoren

Thomas Efferth
Paul Thelen
Hans-Gunther Schulten
Marc-Eric Bode
Bernd Granzen
Antonius Beniers
Rolf Mertens
Rolf-Hermann Ringert
Olaf Gefeller
Gerd Jakse
Laszlo Fuzesi

DOI

10.3892/ijo.19.2.367

eISSN

1791-2423

ISSN

1019-6439

Zeitschrift

International Journal of Oncology

Online publication date

2001

Status

Published online

Herausgeber

Spandidos Publications

Herausgeber URL

http://dx.doi.org/10.3892/ijo.19.2.367

Datum der Datenerfassung

2017

Titel

Differential expression of the multidrug resistance-related protein MRP1 in the histological compartments of nephroblastomas

Data source: Crossref

Abstract

Nephroblastomas (Wilms' tumors) are curable with survival rates above 80%. Some tumors, however, fail to respond to therapy and those patients have a poor prognosis. In a search for prognostic markers, we investigated the expression of the multidrug resistance-related protein 1 (MRP1) in 32 nephroblastomas by means of immunohistochemistry. The immunohistochemical results were validated with a real-time RT-PCR technique. MRP1 expression was heterogeneous and predominantly found in the blastemal and epithelial compartments compared to the stromal elements of nephroblastomas. We found significant relationships of MRP1 expression to survival of patients and to expression of p53, HSP70, and LRP/MVP. The relationship between MRP1 and p53 expression is a clue that the transcriptional control of MRP1 by p53 reported for other tumor types may also take place in nephroblastomas. The correlation of MRP1 to other drug resistance genes, e.g. HSP70 and LRP/MVP in nephroblastomas indicates that the co-expression of different drug resistance genes may be under a common regulation of still unknown transcription factors.

Addresses

Virtual Campus Rhineland-Palatinate, P.O. Box 4380, G-55033 Mainz, Germany. efferth@vcrp.de

Autoren

T Efferth
P Thelen
HG Schulten
ME Bode
B Granzen
AJ Beniers
R Mertens
RH Ringert
O Gefeller
G Jakse
L Fuzesi

DOI

10.3892/ijo.19.2.367

eISSN

1791-2423

Externe Identifier

PubMed Identifier: 11445853

Open access

false

ISSN

1019-6439

Ausgabe der Veröffentlichung

2

Zeitschrift

International journal of oncology

Schlüsselwörter

Vault Ribonucleoprotein Particles
Humans
Kidney Neoplasms
ATP-Binding Cassette Transporters
Multidrug Resistance-Associated Proteins
Neoplasm Proteins
RNA, Messenger
Neoplasm Staging
Immunohistochemistry
Survival Analysis
Reverse Transcriptase Polymerase Chain Reaction
Drug Resistance, Multiple
Gene Expression Regulation, Neoplastic
Wilms Tumor
Tumor Suppressor Protein p53
HSP70 Heat-Shock Proteins

Sprache

eng

Medium

Print

Paginierung

367 - 371

Datum der Veröffentlichung

2001

Status

Published

Datum der Datenerfassung

2001

Titel

Differential expression of the multidrug resistance-related protein MRP1 in the histological compartments of nephroblastomas.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

19

Data source: Europe PubMed Central

Abstract

Nephroblastomas (Wilms' tumors) are curable with survival rates above 80%. Some tumors, however, fail to respond to therapy and those patients have a poor prognosis. In a search for prognostic markers, we investigated the expression of the multidrug resistance-related protein 1 (MRP1) in 32 nephroblastomas by means of immunohistochemistry. The immunohistochemical results were validated with a real-time RT-PCR technique. MRP1 expression was heterogeneous and predominantly found in the blastemal and epithelial compartments compared to the stromal elements of nephroblastomas. We found significant relationships of MRP1 expression to survival of patients and to expression of p53, HSP70, and LRP/MVP. The relationship between MRP1 and p53 expression is a clue that the transcriptional control of MRP1 by p53 reported for other tumor types may also take place in nephroblastomas. The correlation of MRP1 to other drug resistance genes, e.g. HSP70 and LRP/MVP in nephroblastomas indicates that the co-expression of different drug resistance genes may be under a common regulation of still unknown transcription factors.

Autoren

T Efferth
P Thelen
HG Schulten
ME Bode
B Granzen
AJ Beniers
R Mertens
RH Ringert
O Gefeller
G Jakse
L Fuzesi

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/11445853

DOI

10.3892/ijo.19.2.367

ISSN

1019-6439

Ausgabe der Veröffentlichung

2

Zeitschrift

Int J Oncol

Schlüsselwörter

ATP-Binding Cassette Transporters
Drug Resistance, Multiple
Gene Expression Regulation, Neoplastic
HSP70 Heat-Shock Proteins
Humans
Immunohistochemistry
Kidney Neoplasms
Multidrug Resistance-Associated Proteins
Neoplasm Proteins
Neoplasm Staging
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
Tumor Suppressor Protein p53
Vault Ribonucleoprotein Particles
Wilms Tumor

Sprache

eng

Country

Greece

Paginierung

367 - 371

Datum der Veröffentlichung

2001

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2001

Titel

Differential expression of the multidrug resistance-related protein MRP1 in the histological compartments of nephroblastomas.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

19

Data source: PubMed

Differential expression of the multidrug resistance-related protein MRP1 in the histological compartments of nephroblastomas

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