Differential expression of the lung resistance-related protein/major vault protein in the histological compartments of nephroblastomas

Autoren

Thomas Efferth
Marc-Eric Bode
Hans-Gunther Schulten
Paul Thelen
Bernd Granzen
Antonius Beniers
Rolf Mertens
Olaf Gefeller
Rolf-Hermann Ringert
Gerd Jakse
Laszlo Fuzesi

DOI

10.3892/ijo.19.1.163

eISSN

1791-2423

ISSN

1019-6439

Zeitschrift

International Journal of Oncology

Online publication date

2001

Status

Published online

Herausgeber

Spandidos Publications

Herausgeber URL

http://dx.doi.org/10.3892/ijo.19.1.163

Datum der Datenerfassung

2017

Titel

Differential expression of the lung resistance-related protein/major vault protein in the histological compartments of nephroblastomas

Data source: Crossref

Abstract

Nephroblastomas (Wilms' tumors) are curable with survival rates above 80%. Some tumors, however, fail to respond to therapy and those patients have a poor prognosis. In a search for molecular markers of drug resistance, we investigated the expression of lung resistance protein (LRP) in tissue samples from 32 children with nephroblastoma by means of immunohistochemistry. LRP is a human major vault protein (MVP) and is associated with multidrug resistance of tumors. LRP/MVP expression was found in the blastemal and epithelial compartments but to a significantly lesser extent in the stromal compartment of Wilms' tumors. Expression was generally heterogeneous with respect to staining intensity and percentage of positive cells. We found significant relationships between LRP/MVP expression and chemotherapeutic pre-treatment of tumors and tumor stage. The immunohistochemical results were validated with a real-time RT-PCR technique and a significant association between protein and mRNA expression was observed.

Addresses

Virtual Campus Rhineland-Palatinate, D-55033 Mainz, Germany. efferth@vcrp.de

Autoren

T Efferth
ME Bode
HG Schulten
P Thelen
B Granzen
AJ Beniers
R Mertens
O Gefeller
RH Ringert
G Jakse
L Fuzesi

DOI

10.3892/ijo.19.1.163

eISSN

1791-2423

Externe Identifier

PubMed Identifier: 11408938

Open access

false

ISSN

1019-6439

Ausgabe der Veröffentlichung

1

Zeitschrift

International journal of oncology

Schlüsselwörter

Vault Ribonucleoprotein Particles
Humans
Kidney Neoplasms
Vincristine
Dactinomycin
beta 2-Microglobulin
Neoplasm Proteins
RNA, Messenger
DNA Primers
Antibiotics, Antineoplastic
Antineoplastic Agents, Phytogenic
Immunoenzyme Techniques
Reverse Transcriptase Polymerase Chain Reaction
Drug Resistance, Multiple
Child
Child, Preschool
Wilms Tumor
Biomarkers, Tumor

Sprache

eng

Medium

Print

Paginierung

163 - 168

Datum der Veröffentlichung

2001

Status

Published

Datum der Datenerfassung

2001

Titel

Differential expression of the lung resistance-related protein/major vault protein in the histological compartments of nephroblastomas.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

19

Data source: Europe PubMed Central

Abstract

Nephroblastomas (Wilms' tumors) are curable with survival rates above 80%. Some tumors, however, fail to respond to therapy and those patients have a poor prognosis. In a search for molecular markers of drug resistance, we investigated the expression of lung resistance protein (LRP) in tissue samples from 32 children with nephroblastoma by means of immunohistochemistry. LRP is a human major vault protein (MVP) and is associated with multidrug resistance of tumors. LRP/MVP expression was found in the blastemal and epithelial compartments but to a significantly lesser extent in the stromal compartment of Wilms' tumors. Expression was generally heterogeneous with respect to staining intensity and percentage of positive cells. We found significant relationships between LRP/MVP expression and chemotherapeutic pre-treatment of tumors and tumor stage. The immunohistochemical results were validated with a real-time RT-PCR technique and a significant association between protein and mRNA expression was observed.

Autoren

T Efferth
ME Bode
HG Schulten
P Thelen
B Granzen
AJ Beniers
R Mertens
O Gefeller
RH Ringert
G Jakse
L Fuzesi

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/11408938

DOI

10.3892/ijo.19.1.163

ISSN

1019-6439

Ausgabe der Veröffentlichung

1

Zeitschrift

Int J Oncol

Schlüsselwörter

Antibiotics, Antineoplastic
Antineoplastic Agents, Phytogenic
Biomarkers, Tumor
Child
Child, Preschool
DNA Primers
Dactinomycin
Drug Resistance, Multiple
Humans
Immunoenzyme Techniques
Kidney Neoplasms
Neoplasm Proteins
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Vault Ribonucleoprotein Particles
Vincristine
Wilms Tumor
beta 2-Microglobulin

Sprache

eng

Country

Greece

Paginierung

163 - 168

Datum der Veröffentlichung

2001

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2001

Titel

Differential expression of the lung resistance-related protein/major vault protein in the histological compartments of nephroblastomas.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

19

Data source: PubMed

Differential expression of the lung resistance-related protein/major vault protein in the histological compartments of nephroblastomas

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