Differential expression of the drug resistance markers DNA topoisomerase IIα and glutathione S-transferase-π in the histological compartments of Wilms' tumors

Publication type:
Journal article
Metadata:
Autoren
  • B Granzen
  • T Efferth
  • U Keller
  • AJMC Beniers
  • R Mertens
  • G Jakse
  • L Füzesi
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000167876000047&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 418DQ
  • PubMed Identifier: 11299842
ISSN
0250-7005
Ausgabe der Veröffentlichung
1B
Zeitschrift
ANTICANCER RESEARCH
Schlüsselwörter
  • Wilms' tumor
  • drug resistance
  • immunohistochemistry
Paginierung
771 - 776
Datum der Veröffentlichung
2001
Status
Published
Titel
Differential expression of the drug resistance markers DNA topoisomerase IIα and glutathione S-transferase-π in the histological compartments of Wilms' tumors
Sub types
  • Article
Ausgabe der Zeitschrift
21

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
More than 80% of the patients presenting with Wilms' tumor can be cured today. Some patients, however, fail to respond to chemotherapy. The objective of this study was to analyze the immunohistochemical distribution of two markers of cytostatic drug resistance, e.g. DNA topoisomerase II alpha (Topo II alpha) and glutathione S-transferase-pi (GST-pi) in 23 Wilms' tumor patients who had undergone an operation between 1984 and 1997. Eight patients had stage I disease, seven stage II, three stage III, four stage IV, and one stage V disease. Five tumors showed high malignancy histology. Investigations were carried out on formalin-fixed and paraffin-embedded tissue sections using the indirect immunoperoxidase method. Topo II alpha was predominantly present in the epithelial components of the specimens. It was more frequently found in anaplastic tumors. There was no difference in the presence of Topo II alpha in the epithelial components between specimens derived from treated and untreated patients. Topo II alpha was, however, less expressed in the blastemal and stromal elements of specimens after preoperative treatment. If GST-pi was present, it was confined to the epithelial components except for one case. While no expression of GST-pi was found in preoperatively untreated Wilms' tumors, it was present in epithelial compartments in 57% of tumors after chemotherapy. In conclusion, preoperative chemotherapy led to compartment-specific alterations in the expression levels of both markers indicating a contribution to treatment response of Wilms' tumors.
Addresses
  • Department of Children and Adolescents, University Hospital Aachen, Germany.
Autoren
  • B Granzen
  • T Efferth
  • U Keller
  • AJ Beniers
  • R Mertens
  • G Jakse
  • L Füzesi
eISSN
1791-7530
Externe Identifier
  • PubMed Identifier: 11299842
Open access
false
ISSN
0250-7005
Ausgabe der Veröffentlichung
1B
Zeitschrift
Anticancer research
Schlüsselwörter
  • Stromal Cells
  • Epithelial Cells
  • Humans
  • Kidney Neoplasms
  • Isoenzymes
  • DNA Topoisomerases, Type II
  • Glutathione Transferase
  • DNA-Binding Proteins
  • Neoplasm Proteins
  • Antigens, Neoplasm
  • Neoplasm Staging
  • Combined Modality Therapy
  • Chemotherapy, Adjuvant
  • Premedication
  • Nephrectomy
  • Follow-Up Studies
  • Drug Resistance, Neoplasm
  • Child
  • Child, Preschool
  • Infant
  • Female
  • Male
  • Wilms Tumor
  • Neoplastic Stem Cells
Sprache
eng
Medium
Print
Paginierung
771 - 776
Datum der Veröffentlichung
2001
Status
Published
Datum der Datenerfassung
2001
Titel
Differential expression of the drug resistance markers DNA topoisomerase II alpha and glutathione S-transferase-pi in the histological compartments of Wilms' tumors.
Sub types
  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
21

Data source: Europe PubMed Central

Abstract
More than 80% of the patients presenting with Wilms' tumor can be cured today. Some patients, however, fail to respond to chemotherapy. The objective of this study was to analyze the immunohistochemical distribution of two markers of cytostatic drug resistance, e.g. DNA topoisomerase II alpha (Topo II alpha) and glutathione S-transferase-pi (GST-pi) in 23 Wilms' tumor patients who had undergone an operation between 1984 and 1997. Eight patients had stage I disease, seven stage II, three stage III, four stage IV, and one stage V disease. Five tumors showed high malignancy histology. Investigations were carried out on formalin-fixed and paraffin-embedded tissue sections using the indirect immunoperoxidase method. Topo II alpha was predominantly present in the epithelial components of the specimens. It was more frequently found in anaplastic tumors. There was no difference in the presence of Topo II alpha in the epithelial components between specimens derived from treated and untreated patients. Topo II alpha was, however, less expressed in the blastemal and stromal elements of specimens after preoperative treatment. If GST-pi was present, it was confined to the epithelial components except for one case. While no expression of GST-pi was found in preoperatively untreated Wilms' tumors, it was present in epithelial compartments in 57% of tumors after chemotherapy. In conclusion, preoperative chemotherapy led to compartment-specific alterations in the expression levels of both markers indicating a contribution to treatment response of Wilms' tumors.
Autoren
  • B Granzen
  • T Efferth
  • U Keller
  • AJ Beniers
  • R Mertens
  • G Jakse
  • L Füzesi
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/11299842
ISSN
0250-7005
Ausgabe der Veröffentlichung
1B
Zeitschrift
Anticancer Res
Schlüsselwörter
  • Antigens, Neoplasm
  • Chemotherapy, Adjuvant
  • Child
  • Child, Preschool
  • Combined Modality Therapy
  • DNA Topoisomerases, Type II
  • DNA-Binding Proteins
  • Drug Resistance, Neoplasm
  • Epithelial Cells
  • Female
  • Follow-Up Studies
  • Glutathione Transferase
  • Humans
  • Infant
  • Isoenzymes
  • Kidney Neoplasms
  • Male
  • Neoplasm Proteins
  • Neoplasm Staging
  • Neoplastic Stem Cells
  • Nephrectomy
  • Premedication
  • Stromal Cells
  • Wilms Tumor
Sprache
eng
Country
Greece
Paginierung
771 - 776
Datum der Veröffentlichung
2001
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2001
Titel
Differential expression of the drug resistance markers DNA topoisomerase II alpha and glutathione S-transferase-pi in the histological compartments of Wilms' tumors.
Sub types
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
21

Data source: PubMed

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