Differential expression of the heat shock protein 70 in the histological compartments of nephroblastomas

Publication type:
Journal article
Metadata:
Autoren
  • T Efferth
  • HG Schulten
  • P Thelen
  • ME Bode
  • AJMC Beniers
  • B Granzen
  • RH Ringert
  • R Mertens
  • O Gefeller
  • G Jakse
  • L Fuzesi
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000171985000010&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 489HB
  • PubMed Identifier: 11712786
ISSN
0250-7005
Ausgabe der Veröffentlichung
4B
Zeitschrift
ANTICANCER RESEARCH
Schlüsselwörter
  • anaplasia
  • heat shock protein prognosis
  • Wilms' tumor
Paginierung
2915 - 2920
Datum der Veröffentlichung
2001
Status
Published
Titel
Differential expression of the heat shock protein 70 in the histological compartments of nephroblastomas
Sub types
  • Article
Ausgabe der Zeitschrift
21

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
Nephroblastomas (Wilms' tumors) are curable with survival rates above 80%. Nevertheless, some tumors fail to respond to therapy and those patients have a poor prognosis. Prognostic factors for nephroblastomas have still not been satisfactorily explored. In an effort to unravel molecular markers for non-responding nephroblastomas, we investigated by means of immunohistochemistty the expression of heat-shock protein 70 (HSP70) in formalin-fixed and paraffin-embedded tissue samples from 32 children afflicted with nephroblastoma. The results were validated using real-time RT-PCR. HSP70 expression was confined to blastemal and epithelial components, while the tumor stroma was negative. HSP70 expression was greater, if the tumors had been chemotherapeutically treated prior to operation, indicating that cytostatic drugs induce HSP70. Furthermore, high HSP70 expression was confined to tumors from children who survived, whereas tumors from dead patients were negative or weakly-positive for HSP70. Though the number of cases analyzed was small, they provide an indication that HSP70 expression may be of prognostic value.
Addresses
  • University of Göttingen, Germany. efferth@vcrp.de
Autoren
  • T Efferth
  • HG Schulten
  • P Thelen
  • ME Bode
  • AJ Beniers
  • B Granzen
  • RH Ringert
  • R Mertens
  • O Gefeller
  • G Jakse
  • L Fuzesi
eISSN
1791-7530
Externe Identifier
  • PubMed Identifier: 11712786
Open access
false
ISSN
0250-7005
Ausgabe der Veröffentlichung
4B
Zeitschrift
Anticancer research
Schlüsselwörter
  • Stromal Cells
  • Epithelial Cells
  • Humans
  • Kidney Neoplasms
  • Vincristine
  • Dactinomycin
  • Neoplasm Proteins
  • Antineoplastic Combined Chemotherapy Protocols
  • Neoplasm Staging
  • Prognosis
  • Treatment Outcome
  • Combined Modality Therapy
  • Survival Analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Gene Expression Regulation, Neoplastic
  • Drug Resistance, Neoplasm
  • Computer Systems
  • Adolescent
  • Child
  • Child, Preschool
  • Infant
  • Wilms Tumor
  • HSP70 Heat-Shock Proteins
  • Neoplastic Stem Cells
  • Biomarkers, Tumor
Sprache
eng
Medium
Print
Paginierung
2915 - 2920
Datum der Veröffentlichung
2001
Status
Published
Datum der Datenerfassung
2001
Titel
Differential expression of the heat shock protein 70 in the histological compartments of nephroblastomas.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
21

Data source: Europe PubMed Central

Abstract
Nephroblastomas (Wilms' tumors) are curable with survival rates above 80%. Nevertheless, some tumors fail to respond to therapy and those patients have a poor prognosis. Prognostic factors for nephroblastomas have still not been satisfactorily explored. In an effort to unravel molecular markers for non-responding nephroblastomas, we investigated by means of immunohistochemistty the expression of heat-shock protein 70 (HSP70) in formalin-fixed and paraffin-embedded tissue samples from 32 children afflicted with nephroblastoma. The results were validated using real-time RT-PCR. HSP70 expression was confined to blastemal and epithelial components, while the tumor stroma was negative. HSP70 expression was greater, if the tumors had been chemotherapeutically treated prior to operation, indicating that cytostatic drugs induce HSP70. Furthermore, high HSP70 expression was confined to tumors from children who survived, whereas tumors from dead patients were negative or weakly-positive for HSP70. Though the number of cases analyzed was small, they provide an indication that HSP70 expression may be of prognostic value.
Autoren
  • T Efferth
  • HG Schulten
  • P Thelen
  • ME Bode
  • AJ Beniers
  • B Granzen
  • RH Ringert
  • R Mertens
  • O Gefeller
  • G Jakse
  • L Fuzesi
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/11712786
ISSN
0250-7005
Ausgabe der Veröffentlichung
4B
Zeitschrift
Anticancer Res
Schlüsselwörter
  • Adolescent
  • Antineoplastic Combined Chemotherapy Protocols
  • Biomarkers, Tumor
  • Child
  • Child, Preschool
  • Combined Modality Therapy
  • Computer Systems
  • Dactinomycin
  • Drug Resistance, Neoplasm
  • Epithelial Cells
  • Gene Expression Regulation, Neoplastic
  • HSP70 Heat-Shock Proteins
  • Humans
  • Infant
  • Kidney Neoplasms
  • Neoplasm Proteins
  • Neoplasm Staging
  • Neoplastic Stem Cells
  • Prognosis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stromal Cells
  • Survival Analysis
  • Treatment Outcome
  • Vincristine
  • Wilms Tumor
Sprache
eng
Country
Greece
Paginierung
2915 - 2920
Datum der Veröffentlichung
2001
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2001
Titel
Differential expression of the heat shock protein 70 in the histological compartments of nephroblastomas.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
21

Data source: PubMed

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