Expression profile of proteins involved in the xenotransplantability of non-small cell lung cancers into athymic nude mice

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000173462900026&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 514VZ
  • PubMed Identifier: 11788907
ISSN
1019-6439
Ausgabe der Veröffentlichung
2
Zeitschrift
INTERNATIONAL JOURNAL OF ONCOLOGY
Schlüsselwörter
  • non-small cell lung cancer
  • protein expression profile
  • xenotransplantability
  • proliferation
  • angiogenesis
  • apoptosis
  • proto-oncogenes
Paginierung
391 - 395
Datum der Veröffentlichung
2002
Status
Published
Titel
Expression profile of proteins involved in the xenotransplantability of non-small cell lung cancers into athymic nude mice
Sub types
  • Article
Ausgabe der Zeitschrift
20

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
The aim of this study was to evaluate the expression profile of proteins involved in growing of human non-small cell lung cancer (NSCLC) in athymic nude mice. The expressions of 20 gene products in primary NSCLC of 170 patients were analyzed and the proteins were correlated with the transplantability of the carcinomas in nude mice. There was no relationship between xenotransplantability of human non-small cell lung cancer in nude mice and histology, stage or lymph node involvement. Of the analyzed proliferative factors PCNA, cyclin A, cyclin D, cdk2, cdk4 and cell cycle phases only cyclin D, cdk4 and the cell cycle phases were up-regulated in growing carcinomas. There was also a correlation between the apoptotic indices and the take rate in nude mice. Concerning microvessel density and angiogenic factors only VEGF showed a relation to xenotransplantability. Of the proto-oncogenes and suppressor gene products N-RAS, P53, FOS and JUN revealed a relationship to the take rate of NSCLC, while such a relationship was not found with MYC, ERBB-1 and ERBB-2. In a second step, a hierarchical cluster analysis was carried out. The resulting clusters were correlated with the take rate of the carcinomas in nude mice. The expression of JUN, N-RAS, FOS, cyclin D, and cdk4 were significantly different in both groups with non- overlapping confidence intervals. Thus, the up-regulation of the proteins JUN, N-RAS, FOS, cyclin D and cdk4 predicts the growth of NSCLC in nude mice.
Addresses
  • Virtual Campus Rhineland-Palatinate, 55033 Mainz, Germany. efferth@vcrp.de
Autoren
eISSN
1791-2423
Externe Identifier
  • PubMed Identifier: 11788907
Open access
false
ISSN
1019-6439
Ausgabe der Veröffentlichung
2
Zeitschrift
International journal of oncology
Schlüsselwörter
  • Animals
  • Humans
  • Mice
  • Mice, Nude
  • Carcinoma, Non-Small-Cell Lung
  • Lung Neoplasms
  • Cell Cycle Proteins
  • Neoplasm Proteins
  • Transplantation, Heterologous
  • Flow Cytometry
  • Immunohistochemistry
  • Gene Expression Profiling
  • Neoplasm Transplantation
  • Cell Division
  • Apoptosis
  • Graft Survival
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor
  • Oncogenes
  • Middle Aged
Sprache
eng
Medium
Print
Paginierung
391 - 395
Datum der Veröffentlichung
2002
Status
Published
Datum der Datenerfassung
2002
Titel
Expression profile of proteins involved in the xenotransplantability of non-small cell lung cancers into athymic nude mice.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
20

Data source: Europe PubMed Central

Abstract
The aim of this study was to evaluate the expression profile of proteins involved in growing of human non-small cell lung cancer (NSCLC) in athymic nude mice. The expressions of 20 gene products in primary NSCLC of 170 patients were analyzed and the proteins were correlated with the transplantability of the carcinomas in nude mice. There was no relationship between xenotransplantability of human non-small cell lung cancer in nude mice and histology, stage or lymph node involvement. Of the analyzed proliferative factors PCNA, cyclin A, cyclin D, cdk2, cdk4 and cell cycle phases only cyclin D, cdk4 and the cell cycle phases were up-regulated in growing carcinomas. There was also a correlation between the apoptotic indices and the take rate in nude mice. Concerning microvessel density and angiogenic factors only VEGF showed a relation to xenotransplantability. Of the proto-oncogenes and suppressor gene products N-RAS, P53, FOS and JUN revealed a relationship to the take rate of NSCLC, while such a relationship was not found with MYC, ERBB-1 and ERBB-2. In a second step, a hierarchical cluster analysis was carried out. The resulting clusters were correlated with the take rate of the carcinomas in nude mice. The expression of JUN, N-RAS, FOS, cyclin D, and cdk4 were significantly different in both groups with non- overlapping confidence intervals. Thus, the up-regulation of the proteins JUN, N-RAS, FOS, cyclin D and cdk4 predicts the growth of NSCLC in nude mice.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/11788907
ISSN
1019-6439
Ausgabe der Veröffentlichung
2
Zeitschrift
Int J Oncol
Schlüsselwörter
  • Animals
  • Apoptosis
  • Carcinoma, Non-Small-Cell Lung
  • Cell Cycle Proteins
  • Cell Division
  • Flow Cytometry
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor
  • Graft Survival
  • Humans
  • Immunohistochemistry
  • Lung Neoplasms
  • Mice
  • Mice, Nude
  • Middle Aged
  • Neoplasm Proteins
  • Neoplasm Transplantation
  • Oncogenes
  • Transplantation, Heterologous
Sprache
eng
Country
Greece
Paginierung
391 - 395
Datum der Veröffentlichung
2002
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2002
Titel
Expression profile of proteins involved in the xenotransplantability of non-small cell lung cancers into athymic nude mice.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
20

Data source: PubMed

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