Induction of apoptosis, depletion of glutathione, and DNA damage by extracorporeal photochemotherapy and psoralen with exposure to UV light in vitro

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000171984700076&DestLinkType=FullRecord&DestApp=WOS_CPL
eISSN
1791-7530
Externe Identifier
  • Clarivate Analytics Document Solution ID: 489GY
  • PubMed Identifier: 11724354
ISSN
0250-7005
Ausgabe der Veröffentlichung
4A
Zeitschrift
ANTICANCER RESEARCH
Schlüsselwörter
  • flow cytometry
  • polymerase chain reaction
Paginierung
2777 - 2783
Datum der Veröffentlichung
2001
Status
Published
Titel
Induction of apoptosis, depletion of glutathione, and DNA damage by extracorporeal photochemotherapy and psoralen with exposure to UV light <i>in vitro</i>
Sub types
  • Article
Ausgabe der Zeitschrift
21

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
Extra corporeal photochemotherapy (ECPT) is a novel treatment for disorders caused by aberrant T lymphocytes. The effects of ECPT were investigated in mononuclear cells (MNC) of six patients suffering from either Sezary syndrome, mycosis fungoides, systemic sclerosis, pemphigus vulgaris or Hodgkin's disease. ECPT caused moderate to severe induction of apoptosis and depletion of glutathione in the MNC of two out of these six patients. The MNC were then treated with 8-methoxypsoralen (8-MOP) and UV light in vitro and analyzed for apoptosis and glutathione levels. 8-MOP and UV light induced a profile of cellular alterations that is similar to ECPT. In addition, we measured DNA damage by means of a PCR-based methodology. As exemplified by the T-cell receptor-delta and glucose-6-phosphate dehydrogenase genes, DNA damage correlated with induction of apoptosis and depletion of glutathione. It is, therefore, reasonable to propose that UV-induced glutathione depletion contributes to DNA lesions which ultimately account for the onset of apoptosis.
Addresses
  • Virtual Campus Rhineland-Palatinate, Mainz, Germany. efferth@vcrp.de
Autoren
eISSN
1791-7530
Externe Identifier
  • PubMed Identifier: 11724354
Open access
false
ISSN
0250-7005
Ausgabe der Veröffentlichung
4A
Zeitschrift
Anticancer research
Schlüsselwörter
  • Leukocytes, Mononuclear
  • Humans
  • Hodgkin Disease
  • Mycosis Fungoides
  • Sezary Syndrome
  • Scleroderma, Systemic
  • DNA Damage
  • Methoxsalen
  • Glutathione
  • Photopheresis
  • Apoptosis
  • Adult
  • Aged
  • Middle Aged
  • Female
  • Male
Sprache
eng
Medium
Print
Paginierung
2777 - 2783
Datum der Veröffentlichung
2001
Status
Published
Datum der Datenerfassung
2001
Titel
Induction of apoptosis, depletion of glutathione, and DNA damage by extracorporeal photochemotherapy and psoralen with exposure to UV light in vitro.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
21

Data source: Europe PubMed Central

Abstract
Extra corporeal photochemotherapy (ECPT) is a novel treatment for disorders caused by aberrant T lymphocytes. The effects of ECPT were investigated in mononuclear cells (MNC) of six patients suffering from either Sezary syndrome, mycosis fungoides, systemic sclerosis, pemphigus vulgaris or Hodgkin's disease. ECPT caused moderate to severe induction of apoptosis and depletion of glutathione in the MNC of two out of these six patients. The MNC were then treated with 8-methoxypsoralen (8-MOP) and UV light in vitro and analyzed for apoptosis and glutathione levels. 8-MOP and UV light induced a profile of cellular alterations that is similar to ECPT. In addition, we measured DNA damage by means of a PCR-based methodology. As exemplified by the T-cell receptor-delta and glucose-6-phosphate dehydrogenase genes, DNA damage correlated with induction of apoptosis and depletion of glutathione. It is, therefore, reasonable to propose that UV-induced glutathione depletion contributes to DNA lesions which ultimately account for the onset of apoptosis.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/11724354
ISSN
0250-7005
Ausgabe der Veröffentlichung
4A
Zeitschrift
Anticancer Res
Schlüsselwörter
  • Adult
  • Aged
  • Apoptosis
  • DNA Damage
  • Female
  • Glutathione
  • Hodgkin Disease
  • Humans
  • Leukocytes, Mononuclear
  • Male
  • Methoxsalen
  • Middle Aged
  • Mycosis Fungoides
  • Photopheresis
  • Scleroderma, Systemic
  • Sezary Syndrome
Sprache
eng
Country
Greece
Paginierung
2777 - 2783
Datum der Veröffentlichung
2001
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2001
Titel
Induction of apoptosis, depletion of glutathione, and DNA damage by extracorporeal photochemotherapy and psoralen with exposure to UV light in vitro.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
21

Data source: PubMed

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