Activity of Drugs from Traditional Chinese Medicine toward Sensitive and MDR1- or MRP1-Overexpressing Multidrug-Resistant Human CCRF-CEM Leukemia Cells

Publication type:
Journal article
Metadata:
Autoren
  • T Efferth
  • M Davey
  • A Olbrich
  • G Rücker
  • E Gebhart
  • R Davey
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000176362900012&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1006/bcmd.2002.0492
Externe Identifier
  • Clarivate Analytics Document Solution ID: 565HC
  • PubMed Identifier: 12064912
ISSN
1079-9796
Ausgabe der Veröffentlichung
2
Zeitschrift
BLOOD CELLS MOLECULES AND DISEASES
Schlüsselwörter
  • P-glycoprotein
  • multidrug resistance-related protein 1
  • traditional Chinese medicine
  • chemotherapy
Paginierung
160 - 168
Datum der Veröffentlichung
2002
Status
Published
Titel
Activity of drugs from traditional Chinese medicine toward sensitive and MDR1- or MRP1-overexpressing multidrug-resistant human CCRF-CEM leukemia cells
Sub types
  • Article
Ausgabe der Zeitschrift
28

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Thomas Efferth
  • Mary Davey
  • Armin Olbrich
  • Gerhard Rücker
  • Erich Gebhart
  • Ross Davey
DOI
10.1006/bcmd.2002.0492
ISSN
1079-9796
Ausgabe der Veröffentlichung
2
Zeitschrift
Blood Cells, Molecules, and Diseases
Sprache
en
Paginierung
160 - 168
Datum der Veröffentlichung
2002
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1006/bcmd.2002.0492
Datum der Datenerfassung
2019
Titel
Activity of Drugs from Traditional Chinese Medicine toward Sensitive and MDR1- or MRP1-Overexpressing Multidrug-Resistant Human CCRF-CEM Leukemia Cells
Ausgabe der Zeitschrift
28

Data source: Crossref

Abstract
There is considerable interest among basic and clinical researchers in novel drugs with activity against leukemia. The vast history of experience of traditional Chinese medicine (TCM) with medicinal plants may facilitate the identification of novel antileukemic compounds. In the present investigation, we tested 22 drugs for their activity toward CCRF-CEM cell lines: artesunate, artemisinin, baicalein, baicalin, berberine, bufalin, cantharidin, cephalotaxine, curcumin, daidzein, daidzin, diallyl disulfide, ginsenoside Rh2, glycyrrhizic acid, isonardosinon, homoharringtonine, nardosinon, nardofuran, puerarin, quercetin, tannic acid, and tetrahydronardosinon. As compounds from folk medicinal remedies are sometimes looked upon as alternative medicine with some hesitation or criticism, we investigated only chemically pure compounds and tested the drugs independently in two different laboratories in Germany and Australia. We used CCRF-CEM parental cells and doxorubicin-selected P-glycoprotein (P-gp)/MDR1-expressing CEM/ADR5000, vinblastine-selected P-gp/MDR1-expressing CEM/VLB(100), and epirubicin-selected multidrug resistance-related protein 1 (MRP1)-expressing CEM/E1000 sublines thereof. While CEM/ADR5000, CEM/VLB(100), and CEM/E1000 cells were highly resistant to the corresponding selecting agents, no or only minimal degrees of cross-resistance were observed to TCM drugs in both growth inhibition assay and MTT assay (range from 0.4- to 8-fold). Homoharringtonine, artesunate, and bufalin were most active among this panel of compounds. As shown by flow cytometry, artesunate significantly increased daunorubicin accumulation in CEM/E1000 cells, but not in CEM/VLB(100) or CCRF-CEM parental cells. Bufalin caused a small, but significant increase in daunorubicin accumulation in CEM/VLB(100) and CEM/E1000 cells. As artesunate and bufalin showed both antileukemic activity if applied alone and modulation activity in combination with daunorubicin in multidrug-resistant (MDR) cells, these two drugs may be suitable for novel combination treatment regimens to improve leukemia cell killing.
Addresses
  • Virtual Campus Rhineland-Palatinate, Mainz, Germany. efferth@vcrp.de
Autoren
  • Thomas Efferth
  • Thomas Efferth
  • Mary Davey
  • Armin Olbrich
  • Gerhard Rücker
  • Erich Gebhart
  • Ross Davey
DOI
10.1006/bcmd.2002.0492
eISSN
1096-0961
Externe Identifier
  • PubMed Identifier: 12064912
Open access
false
ISSN
1079-9796
Ausgabe der Veröffentlichung
2
Zeitschrift
Blood cells, molecules & diseases
Schlüsselwörter
  • Tumor Cells, Cultured
  • Humans
  • Daunorubicin
  • Multidrug Resistance-Associated Proteins
  • Drugs, Chinese Herbal
  • Medicine, Chinese Traditional
  • Drug Screening Assays, Antitumor
  • Drug Resistance, Multiple
  • Cell Division
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Drug Resistance, Neoplasm
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
Sprache
eng
Medium
Print
Paginierung
160 - 168
Datum der Veröffentlichung
2002
Status
Published
Datum der Datenerfassung
2002
Titel
Activity of drugs from traditional Chinese medicine toward sensitive and MDR1- or MRP1-overexpressing multidrug-resistant human CCRF-CEM leukemia cells.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
28

Data source: Europe PubMed Central

Abstract
There is considerable interest among basic and clinical researchers in novel drugs with activity against leukemia. The vast history of experience of traditional Chinese medicine (TCM) with medicinal plants may facilitate the identification of novel antileukemic compounds. In the present investigation, we tested 22 drugs for their activity toward CCRF-CEM cell lines: artesunate, artemisinin, baicalein, baicalin, berberine, bufalin, cantharidin, cephalotaxine, curcumin, daidzein, daidzin, diallyl disulfide, ginsenoside Rh2, glycyrrhizic acid, isonardosinon, homoharringtonine, nardosinon, nardofuran, puerarin, quercetin, tannic acid, and tetrahydronardosinon. As compounds from folk medicinal remedies are sometimes looked upon as alternative medicine with some hesitation or criticism, we investigated only chemically pure compounds and tested the drugs independently in two different laboratories in Germany and Australia. We used CCRF-CEM parental cells and doxorubicin-selected P-glycoprotein (P-gp)/MDR1-expressing CEM/ADR5000, vinblastine-selected P-gp/MDR1-expressing CEM/VLB(100), and epirubicin-selected multidrug resistance-related protein 1 (MRP1)-expressing CEM/E1000 sublines thereof. While CEM/ADR5000, CEM/VLB(100), and CEM/E1000 cells were highly resistant to the corresponding selecting agents, no or only minimal degrees of cross-resistance were observed to TCM drugs in both growth inhibition assay and MTT assay (range from 0.4- to 8-fold). Homoharringtonine, artesunate, and bufalin were most active among this panel of compounds. As shown by flow cytometry, artesunate significantly increased daunorubicin accumulation in CEM/E1000 cells, but not in CEM/VLB(100) or CCRF-CEM parental cells. Bufalin caused a small, but significant increase in daunorubicin accumulation in CEM/VLB(100) and CEM/E1000 cells. As artesunate and bufalin showed both antileukemic activity if applied alone and modulation activity in combination with daunorubicin in multidrug-resistant (MDR) cells, these two drugs may be suitable for novel combination treatment regimens to improve leukemia cell killing.
Autoren
  • Thomas Efferth
  • Mary Davey
  • Armin Olbrich
  • Gerhard Rücker
  • Erich Gebhart
  • Ross Davey
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/12064912
DOI
10.1006/bcmd.2002.0492
ISSN
1079-9796
Ausgabe der Veröffentlichung
2
Zeitschrift
Blood Cells Mol Dis
Schlüsselwörter
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Cell Division
  • Daunorubicin
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Drug Screening Assays, Antitumor
  • Drugs, Chinese Herbal
  • Humans
  • Medicine, Chinese Traditional
  • Multidrug Resistance-Associated Proteins
  • Tumor Cells, Cultured
Sprache
eng
Country
United States
Paginierung
160 - 168
PII
S1079979602904924
Datum der Veröffentlichung
2002
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2003
Titel
Activity of drugs from traditional Chinese medicine toward sensitive and MDR1- or MRP1-overexpressing multidrug-resistant human CCRF-CEM leukemia cells.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
28

Data source: PubMed

Autoren
  • T EFFERTH
Zeitschrift
Blood Cells Mol. Dis.
Paginierung
160 - 168
Datum der Veröffentlichung
2002
Ausgabe der Zeitschrift
28

Data source: CiNii EN

Autoren
  • T EFFERTH
Zeitschrift
Blood Cells Mol. Dis.
Paginierung
160 - 168
Datum der Veröffentlichung
2002
Ausgabe der Zeitschrift
28

Data source: CiNii JP

Autoren
  • T EFFERTH
Zeitschrift
Blood Cells Mol. Dis.
Paginierung
160 - 168
Datum der Veröffentlichung
2002
Datum der Datenerfassung
2022
Titel
Activity of Drugs from Traditional Chinese Medicine toward Sensitive and MDR1- or MRP1-Overexpressing Multidrug-Resistant Human CCRF-CEM Leukemia Cells
Ausgabe der Zeitschrift
28

Data source: Manual

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