Specific patterns of DNA copy number gains and losses in eight new glioblastoma multiforme cell lines

Publication type:
Journal article
Metadata:
Autoren
  • T Ramirez
  • K Thoma
  • L Taja-Chayeb
  • T Efferth
  • LA Herrera
  • ME Halatsch
  • E Gebhart
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000184195600022&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 701YB
  • PubMed Identifier: 12851695
ISSN
1019-6439
Ausgabe der Veröffentlichung
2
Zeitschrift
INTERNATIONAL JOURNAL OF ONCOLOGY
Schlüsselwörter
  • glioblastoma multiforme
  • comparative genomic hybridization
  • DNA copy number alterations
Paginierung
453 - 460
Datum der Veröffentlichung
2003
Status
Published
Titel
Specific patterns of DNA copy number gains and losses in eight new glioblastoma multiforme cell lines
Sub types
  • Article
Ausgabe der Zeitschrift
23

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
Eight cell lines newly established from glioblastoma multiforme were examined by comparative genomic hybridization for their patterns of genomic imbalance. The total number of DNA copy number alterations (CNAs) found in the eight cell lines varied between 15 and 24. This characterized the examined cell lines (or the tumors they were derived from) as distinctly progressed in karyotypic evolution. The most frequent CNAs were gains of the entire chromosome 6 or, at least, parts of it, and of 7p22, which were found in all eight cell lines. Other changes present in seven of the eight cell lines were gains of 3q26qter and the entire chromosome 7 and losses of segments on chromosome 4q (e.g., 4q34q35) and of the short arm of chromosome 10. Enh(3q21q25), dim(4q22q33) and dim(4qter), dim(13q22), enh(15q14), and enh(18q22q23) were found in six of the eight cell lines. Several other CNAs [e.g., dim(9p21)] were found in common in five or less of the eight lines. Using a hierarchical cluster analysis, the specific patterns of genomic imbalance allowed various groupings of the examined cell lines. Although a close relation could be confirmed among all examined lines on the basis of shared CNAs, two main groups could be roughly differentiated. Among those there were also more or less closely related subgroups. However, also alterations which were restricted to one single cell line each were found, e.g., dim(1q41qter), dim(2q22qter), enh(4p), dim(5p), dim(4p13pter), dim(8q21qter), enh(9p), dim(9q), dim(11p14pter), enh(12q15q23), enh(13q21), dim(14q21qter), dim(15q21qter), dim(19q), and enh(22q). The comparison of the obtained data on gains and losses of DNA copy numbers in specific chromosomal segments with the data on localization of genes possibly associated with the biology of glioblastoma multiforme additionally shows high conformity but also disparity of the examined cell lines among each other, as well as compared to primary glioblastoma multiforme. Eventually, each of the cell lines could be characterized by its specific pattern of genomic imbalance.
Addresses
  • Institute of Human Genetics, University of Erlangen-Nuremberg, D-91054 Erlangen, Germany.
Autoren
  • Tzutzuy Ramirez
  • Karen Thoma
  • Lucia Taja-Chayeb
  • Thomas Efferth
  • Luis A Herrera
  • Marc-Eric Halatsch
  • Erich Gebhart
eISSN
1791-2423
Externe Identifier
  • PubMed Identifier: 12851695
Open access
false
ISSN
1019-6439
Ausgabe der Veröffentlichung
2
Zeitschrift
International journal of oncology
Schlüsselwörter
  • Tumor Cells, Cultured
  • Chromosomes, Human
  • Humans
  • Glioblastoma
  • Chromosome Aberrations
  • Neoplasm Proteins
  • DNA, Neoplasm
  • Chromosome Mapping
  • Karyotyping
  • Nucleic Acid Hybridization
  • Gene Dosage
  • Aged
  • Middle Aged
  • Female
  • Male
Sprache
eng
Medium
Print
Paginierung
453 - 460
Datum der Veröffentlichung
2003
Status
Published
Datum der Datenerfassung
2003
Titel
Specific patterns of DNA copy number gains and losses in eight new glioblastoma multiforme cell lines.
Sub types
  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
23

Data source: Europe PubMed Central

Abstract
Eight cell lines newly established from glioblastoma multiforme were examined by comparative genomic hybridization for their patterns of genomic imbalance. The total number of DNA copy number alterations (CNAs) found in the eight cell lines varied between 15 and 24. This characterized the examined cell lines (or the tumors they were derived from) as distinctly progressed in karyotypic evolution. The most frequent CNAs were gains of the entire chromosome 6 or, at least, parts of it, and of 7p22, which were found in all eight cell lines. Other changes present in seven of the eight cell lines were gains of 3q26qter and the entire chromosome 7 and losses of segments on chromosome 4q (e.g., 4q34q35) and of the short arm of chromosome 10. Enh(3q21q25), dim(4q22q33) and dim(4qter), dim(13q22), enh(15q14), and enh(18q22q23) were found in six of the eight cell lines. Several other CNAs [e.g., dim(9p21)] were found in common in five or less of the eight lines. Using a hierarchical cluster analysis, the specific patterns of genomic imbalance allowed various groupings of the examined cell lines. Although a close relation could be confirmed among all examined lines on the basis of shared CNAs, two main groups could be roughly differentiated. Among those there were also more or less closely related subgroups. However, also alterations which were restricted to one single cell line each were found, e.g., dim(1q41qter), dim(2q22qter), enh(4p), dim(5p), dim(4p13pter), dim(8q21qter), enh(9p), dim(9q), dim(11p14pter), enh(12q15q23), enh(13q21), dim(14q21qter), dim(15q21qter), dim(19q), and enh(22q). The comparison of the obtained data on gains and losses of DNA copy numbers in specific chromosomal segments with the data on localization of genes possibly associated with the biology of glioblastoma multiforme additionally shows high conformity but also disparity of the examined cell lines among each other, as well as compared to primary glioblastoma multiforme. Eventually, each of the cell lines could be characterized by its specific pattern of genomic imbalance.
Autoren
  • Tzutzuy Ramirez
  • Karen Thoma
  • Lucia Taja-Chayeb
  • Thomas Efferth
  • Luis A Herrera
  • Marc-Eric Halatsch
  • Erich Gebhart
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/12851695
ISSN
1019-6439
Ausgabe der Veröffentlichung
2
Zeitschrift
Int J Oncol
Schlüsselwörter
  • Aged
  • Chromosome Aberrations
  • Chromosome Mapping
  • Chromosomes, Human
  • DNA, Neoplasm
  • Female
  • Gene Dosage
  • Glioblastoma
  • Humans
  • Karyotyping
  • Male
  • Middle Aged
  • Neoplasm Proteins
  • Nucleic Acid Hybridization
  • Tumor Cells, Cultured
Sprache
eng
Country
Greece
Paginierung
453 - 460
Datum der Veröffentlichung
2003
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2004
Titel
Specific patterns of DNA copy number gains and losses in eight new glioblastoma multiforme cell lines.
Sub types
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
23

Data source: PubMed

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