EGFR but not PDGFR-β expression correlates to the antiproliferative effect of growth factor withdrawal in glioblastoma multiforme cell lines

Publication type:
Journal article
Metadata:
Autoren
  • ME Halatsch
  • E Gehrke
  • F A-Borhani
  • T Efferth
  • C Werner
  • P Nomikos
  • U Schmidt
  • M Buchfelder
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000184359200016&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 704UP
  • PubMed Identifier: 12894509
ISSN
0250-7005
Ausgabe der Veröffentlichung
3B
Zeitschrift
ANTICANCER RESEARCH
Schlüsselwörter
  • glioblastoma multiforme
  • epidermal growth factor receptor
  • platelet-derived growth factor receptor-beta
  • growth factor withdrawal
  • proliferation
  • tumorigenicity
Paginierung
2315 - 2320
Datum der Veröffentlichung
2003
Status
Published
Titel
EGFR but not PDGFR-β expression correlates to the antiproliferative effect of growth factor withdrawal in glioblastoma multiforme cell lines
Sub types
  • Article
Ausgabe der Zeitschrift
23

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<h4>Background</h4>The aim of the current study was to investigate a putative relationship between (i) growth characteristics (proliferation and tumorigenicity) of nine glioblastoma multiforme (GBM) cell lines under different growth-stimulating conditions in vitro and (ii) their basal expression of a panel of growth factor receptors/autocrine cytokines.<h4>Materials and methods</h4>Basal expressions of the epidermal growth factor receptor (EGFR), platelet-derived growth factor receptor-beta (PDGFR-beta), platelet-derived growth factor-AA (PDGF-AA) and PDGF-BB, tumor growth factor-alpha (TGF-alpha) and TGF-beta as well as tumor necrosis factor-alpha (TNF-alpha) were determined by immunocytochemistry at standard cell culture conditions (10% fetal calf serum [FCS]). Proliferation and tumorigenicity at 10% FCS and relative serum starvation (0.5% FCS) were assessed by using Coulter counting and soft agar cloning, respectively.<h4>Results</h4>The ratio between cell multiplications at 10% and 0.5% FCS over a 10-day period was defined as a measure of growth factor dependence of cellular proliferation. Expression of EGFR (but not of PDGFR-beta) strongly correlated to this ratio (Spearman rank correlation coefficient R = 0.87). No considerable correlations were present among other appropriate pairs of variables with biologically founded putative relationships.<h4>Conclusion</h4>Greater expression of EGFR is associated with increased growth factor dependence of cellular proliferation. Our results strengthen the role of EGFR as a rational molecular target of therapeutic intervention in GBM.
Addresses
  • Department of Neurosurgery, Georg-August-University, Robert-Koch-Strasse 40, D-37075 Göttingen, Germany. halatsch@aol.com
Autoren
  • Marc-E Halatsch
  • Esther Gehrke
  • Farhad A Borhani
  • Thomas Efferth
  • Carola Werner
  • Panos Nomikos
  • Ursula Schmidt
  • Michael Buchfelder
eISSN
1791-7530
Externe Identifier
  • PubMed Identifier: 12894509
Open access
false
ISSN
0250-7005
Ausgabe der Veröffentlichung
3B
Zeitschrift
Anticancer research
Schlüsselwörter
  • Tumor Cells, Cultured
  • Humans
  • Glioblastoma
  • Receptor, Platelet-Derived Growth Factor beta
  • Platelet-Derived Growth Factor
  • Proto-Oncogene Proteins c-sis
  • Transforming Growth Factor alpha
  • Transforming Growth Factor beta
  • Tumor Necrosis Factor-alpha
  • Immunohistochemistry
  • Cell Division
  • ErbB Receptors
  • Becaplermin
Sprache
eng
Medium
Print
Paginierung
2315 - 2320
Datum der Veröffentlichung
2003
Status
Published
Datum der Datenerfassung
2003
Titel
EGFR but not PDGFR-beta expression correlates to the antiproliferative effect of growth factor withdrawal in glioblastoma multiforme cell lines.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
23

Data source: Europe PubMed Central

Abstract
BACKGROUND: The aim of the current study was to investigate a putative relationship between (i) growth characteristics (proliferation and tumorigenicity) of nine glioblastoma multiforme (GBM) cell lines under different growth-stimulating conditions in vitro and (ii) their basal expression of a panel of growth factor receptors/autocrine cytokines. MATERIALS AND METHODS: Basal expressions of the epidermal growth factor receptor (EGFR), platelet-derived growth factor receptor-beta (PDGFR-beta), platelet-derived growth factor-AA (PDGF-AA) and PDGF-BB, tumor growth factor-alpha (TGF-alpha) and TGF-beta as well as tumor necrosis factor-alpha (TNF-alpha) were determined by immunocytochemistry at standard cell culture conditions (10% fetal calf serum [FCS]). Proliferation and tumorigenicity at 10% FCS and relative serum starvation (0.5% FCS) were assessed by using Coulter counting and soft agar cloning, respectively. RESULTS: The ratio between cell multiplications at 10% and 0.5% FCS over a 10-day period was defined as a measure of growth factor dependence of cellular proliferation. Expression of EGFR (but not of PDGFR-beta) strongly correlated to this ratio (Spearman rank correlation coefficient R = 0.87). No considerable correlations were present among other appropriate pairs of variables with biologically founded putative relationships. CONCLUSION: Greater expression of EGFR is associated with increased growth factor dependence of cellular proliferation. Our results strengthen the role of EGFR as a rational molecular target of therapeutic intervention in GBM.
Autoren
  • Marc-E Halatsch
  • Esther Gehrke
  • Farhad A Borhani
  • Thomas Efferth
  • Carola Werner
  • Panos Nomikos
  • Ursula Schmidt
  • Michael Buchfelder
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/12894509
ISSN
0250-7005
Ausgabe der Veröffentlichung
3B
Zeitschrift
Anticancer Res
Schlüsselwörter
  • Becaplermin
  • Cell Division
  • ErbB Receptors
  • Glioblastoma
  • Humans
  • Immunohistochemistry
  • Platelet-Derived Growth Factor
  • Proto-Oncogene Proteins c-sis
  • Receptor, Platelet-Derived Growth Factor beta
  • Transforming Growth Factor alpha
  • Transforming Growth Factor beta
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha
Sprache
eng
Country
Greece
Paginierung
2315 - 2320
Datum der Veröffentlichung
2003
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2003
Titel
EGFR but not PDGFR-beta expression correlates to the antiproliferative effect of growth factor withdrawal in glioblastoma multiforme cell lines.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
23

Data source: PubMed

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