Role of antioxidant genes for the activity of artesunate against tumor cells

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000185958100046&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 732QY
  • PubMed Identifier: 12964009
ISSN
1019-6439
Ausgabe der Veröffentlichung
4
Zeitschrift
INTERNATIONAL JOURNAL OF ONCOLOGY
Schlüsselwörter
  • artesunate
  • bcl-2
  • catalase
  • false discovery rate calculation
  • hydrogen peroxide
  • microarray
  • superoxide dismutase
  • thioredoxin
Paginierung
1231 - 1235
Datum der Veröffentlichung
2003
Status
Published
Titel
Role of antioxidant genes for the activity of artesunate against tumor cells
Sub types
  • Article
Ausgabe der Zeitschrift
23

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
The antimalaria drug, artesunate (ART), is very cytotoxic in tumor cell lines. The active moiety of ART is an endoperoxide bridge that generates carbon-centered free radicals and oxidative stress upon cleavage. Oxidative stress appears to be necessary for the antimalarial activity of ART. To test whether antioxidant gene expression affects the ART response in tumor cell lines we compared the baseline antioxidant mRNA gene expression in the 55 human tumor cell line panel from the National Cancer Institute Developmental Therapeutics Program to the ART IC50. Thioredoxin reductase expression showed a significant positive correlation to the ART IC50 and catalase expression was inversely correlated with the ART IC50 (p<0.05). WEHI7.2 mouse thymoma cells selected for resistance to hydrogen peroxide or transfected with thioredoxin, manganese superoxide dismutase, catalase or bcl-2 showed resistance to ART compared to the parental cell line. Taken together these data support a role for oxidative stress in the mechanism of ART action in tumor cells and suggest that antioxidant defenses act in combination to affect the cellular response to ART.
Addresses
  • Center for Molecular Biology at the University of Heidelberg (ZMBH), 69120 Heidelberg, Germany. thomas.efferth@web.de
Autoren
eISSN
1791-2423
Externe Identifier
  • PubMed Identifier: 12964009
Open access
false
ISSN
1019-6439
Ausgabe der Veröffentlichung
4
Zeitschrift
International journal of oncology
Schlüsselwörter
  • Cell Line, Tumor
  • Animals
  • Humans
  • Mice
  • Thymoma
  • Hydrogen Peroxide
  • Carbon
  • Sesquiterpenes
  • Artemisinins
  • Catalase
  • Superoxide Dismutase
  • DNA, Complementary
  • RNA, Messenger
  • Antioxidants
  • Transfection
  • Inhibitory Concentration 50
  • Oxidative Stress
  • Thioredoxins
  • Artesunate
Sprache
eng
Medium
Print
Paginierung
1231 - 1235
Datum der Veröffentlichung
2003
Status
Published
Datum der Datenerfassung
2003
Titel
Role of antioxidant genes for the activity of artesunate against tumor cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
23

Data source: Europe PubMed Central

Abstract
The antimalaria drug, artesunate (ART), is very cytotoxic in tumor cell lines. The active moiety of ART is an endoperoxide bridge that generates carbon-centered free radicals and oxidative stress upon cleavage. Oxidative stress appears to be necessary for the antimalarial activity of ART. To test whether antioxidant gene expression affects the ART response in tumor cell lines we compared the baseline antioxidant mRNA gene expression in the 55 human tumor cell line panel from the National Cancer Institute Developmental Therapeutics Program to the ART IC50. Thioredoxin reductase expression showed a significant positive correlation to the ART IC50 and catalase expression was inversely correlated with the ART IC50 (p<0.05). WEHI7.2 mouse thymoma cells selected for resistance to hydrogen peroxide or transfected with thioredoxin, manganese superoxide dismutase, catalase or bcl-2 showed resistance to ART compared to the parental cell line. Taken together these data support a role for oxidative stress in the mechanism of ART action in tumor cells and suggest that antioxidant defenses act in combination to affect the cellular response to ART.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/12964009
ISSN
1019-6439
Ausgabe der Veröffentlichung
4
Zeitschrift
Int J Oncol
Schlüsselwörter
  • Animals
  • Antioxidants
  • Artemisinins
  • Artesunate
  • Carbon
  • Catalase
  • Cell Line, Tumor
  • DNA, Complementary
  • Humans
  • Hydrogen Peroxide
  • Inhibitory Concentration 50
  • Mice
  • Oxidative Stress
  • RNA, Messenger
  • Sesquiterpenes
  • Superoxide Dismutase
  • Thioredoxins
  • Thymoma
  • Transfection
Sprache
eng
Country
Greece
Paginierung
1231 - 1235
Datum der Veröffentlichung
2003
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2004
Titel
Role of antioxidant genes for the activity of artesunate against tumor cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
23

Data source: PubMed

Beziehungen:
Property of

Tools