Inverse correlation of epidermal growth factor receptor messenger RNA induction and suppression of anchorage-independent growth by OSI-774, an epidermal growth factor receptor tyrosine kinase inhibitor, in glioblastoma multiforme cell lines
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- ME Halatsch
- EE Gehrke
- VI Vougioukas
- IC Bötefür
- FA Borhani
- T Efferth
- E Gebhart
- S Domhof
- U Schmidt
- M Buchfelder
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000220186400025&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3171/jns.2004.100.3.0523
- Externe Identifier
- Clarivate Analytics Document Solution ID: 802TS
- PubMed Identifier: 15035290
- ISSN
- 0022-3085
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- JOURNAL OF NEUROSURGERY
- Schlüsselwörter
- glioblastoma multiforme
- epidermal growth factor receptor
- tyrosine kinase inhibitor
- proliferation
- anchorage-independent growth
- apoptosis
- Paginierung
- 523 - 533
- Datum der Veröffentlichung
- 2004
- Status
- Published
- Titel
- Inverse correlation of <i>epidermal growth factor receptor</i> messenger RNA induction and suppression of anchorage-independent growth by OSI-774, an epidermal growth factor receptor tyrosine kinase inhibitor, in glioblastoma multiforme cell lines
- Sub types
- Article
- Proceedings Paper
- Ausgabe der Zeitschrift
- 100
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p content-type="fine-print"><jats:italic>Object.</jats:italic>Quantitative and qualitative alterations in the epidermal growth factor receptor (EGFR) commonly occur in many cancers in humans, including malignant gliomas. The aim of the current study was to evaluate molecular and cellular effects of OSI-774, a novel EGFR tyrosine kinase inhibitor, on nine glioblastoma multiforme (GBM) cell lines.</jats:p><jats:p content-type="fine-print"><jats:italic>Methods.</jats:italic>The effects of OSI-774 on expression of<jats:italic>EGFR</jats:italic>messenger (m)RNA and protein, proliferation, anchorage-independent growth, and apoptosis were examined using semiquantitative reverse transcription—polymerase chain reaction, immunocytochemical analysis, Coulter counting, soft agar cloning, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling/fluorescence-activated cell sorting, respectively. All<jats:italic>p53</jats:italic>genes were completely and bidirectionally sequenced.</jats:p><jats:p content-type="fine-print">Suppression of anchorage-independent growth by OSI-774 was inversely correlated to the induction of<jats:italic>EGFR</jats:italic>mRNA during relative serum starvation (r = −0.74) and was unrelated to<jats:italic>p53</jats:italic>status. Overall, suppression of anchorage-independent growth was a considerably stronger effect of OSI-774 than inhibition of proliferation. The extent of OSI-774—induced apoptosis positively correlated with both proliferation and anchorage-independent growth of GBM cell lines (r = 0.75 and 0.79, respectively). In a single cell line derived from a secondary GBM, exposure to concentrations of greater than or equal to 1 Émol/L resulted in a substantial net cell loss during proliferation studies.</jats:p><jats:p content-type="fine-print"><jats:italic>Conclusions.</jats:italic>The induction of<jats:italic>EGFR</jats:italic>mRNA may constitute a cellular mechanism to counteract the inhibitory effect of OSI-774 on the anchorage-independent growth of GBM cells. In contrast, no considerable correlation could be established between baseline expression levels of EGFR (both mRNA and protein) in GBM cell lines and their biological response to OSI-774. The OSI-774 induced greater (<jats:italic>p53</jats:italic>-independent) apoptosis in more malignant GBM phenotypes and may be a promising therapeutic agent against secondary GBM.</jats:p>
- Autoren
- Marc-Eric Halatsch
- Esther E Gehrke
- Vassilios I Vougioukas
- Ingolf C Bötefür
- Farhad A.-Borhani
- Thomas Efferth
- Erich Gebhart
- Sebastian Domhof
- Ursula Schmidt
- Michael Buchfelder
- DOI
- 10.3171/jns.2004.100.3.0523
- ISSN
- 0022-3085
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Journal of Neurosurgery
- Paginierung
- 523 - 533
- Datum der Veröffentlichung
- 2004
- Status
- Published
- Herausgeber
- Journal of Neurosurgery Publishing Group (JNSPG)
- Herausgeber URL
- http://dx.doi.org/10.3171/jns.2004.100.3.0523
- Datum der Datenerfassung
- 2023
- Titel
- Inverse correlation of epidermal growth factor receptor messenger RNA induction and suppression of anchorage-independent growth by OSI-774, an epidermal growth factor receptor tyrosine kinase inhibitor, in glioblastoma multiforme cell lines
- Ausgabe der Zeitschrift
- 100
Data source: Crossref
- Abstract
- <h4>Object</h4>Quantitative and qualitative alterations in the epidermal growth factor receptor (EGFR) commonly occur in many cancers in humans, including malignant gliomas. The aim of the current study was to evaluate molecular and cellular effects of OSI-774, a novel EGFR tyrosine kinase inhibitor, on nine glioblastoma multiforme (GBM) cell lines.<h4>Methods</h4>The effects of OSI-774 on expression of EGFR messenger (m)RNA and protein, proliferation, anchorage-independent growth, and apoptosis were examined using semiquantitative reverse transcription-polymerase chain reaction, immunocytochemical analysis, Coulter counting, soft agar cloning, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling/fluorescence-activated cell sorting, respectively. All p53 genes were completely and bidirectionally sequenced. Suppression of anchorage-independent growth by OSI-774 was inversely correlated to the induction of EGFR mRNA during relative serum starvation (r = -0.74) and was unrelated to p53 status. Overall, suppression of anchorage-independent growth was a considerably stronger effect of OSI-774 than inhibition of proliferation. The extent of OSI-774-induced apoptosis positively correlated with both proliferation and anchorage-independent growth of GBM cell lines (r = 0.75 and 0.79, respectively). In a single cell line derived from a secondary GBM, exposure to concentrations of greater than or equal to 1 micromol/L resulted in a substantial net cell loss during proliferation studies.<h4>Conclusions</h4>The induction of EGFR mRNA may constitute a cellular mechanism to counteract the inhibitory effect of OSI-774 on the anchorage-independent growth of GBM cells. In contrast, no considerable correlation could be established between baseline expression levels of EGFR (both mRNA and protein) in GBM cell lines and their biological response to OSI-774. The OSI-774 induced greater (p53-independent) apoptosis in more malignant GBM phenotypes and may be a promising therapeutic agent against secondary GBM.
- Addresses
- Klinik und Poliklinik für Neurochirurgie, Georg-August-Universität, Göttingen, Germany. halatsch@aol.com
- Autoren
- Marc-Eric Halatsch
- Esther E Gehrke
- Vassilios I Vougioukas
- Ingolf C Bötefür
- Farhad A-Borhani
- Thomas Efferth
- Thomas Efferth
- Erich Gebhart
- Sebastian Domhof
- Ursula Schmidt
- Michael Buchfelder
- DOI
- 10.3171/jns.2004.100.3.0523
- eISSN
- 1933-0693
- Externe Identifier
- PubMed Identifier: 15035290
- Open access
- false
- ISSN
- 0022-3085
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Journal of neurosurgery
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Glioblastoma
- Quinazolines
- Actins
- DNA, Complementary
- RNA, Messenger
- DNA Primers
- Immunohistochemistry
- Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Cell Division
- Apoptosis
- Cell Movement
- Genes, p53
- ErbB Receptors
- Erlotinib Hydrochloride
- Sprache
- eng
- Medium
- Paginierung
- 523 - 533
- Datum der Veröffentlichung
- 2004
- Status
- Published
- Datum der Datenerfassung
- 2004
- Titel
- Inverse correlation of epidermal growth factor receptor messenger RNA induction and suppression of anchorage-independent growth by OSI-774, an epidermal growth factor receptor tyrosine kinase inhibitor, in glioblastoma multiforme cell lines.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 100
Data source: Europe PubMed Central
- Abstract
- OBJECT: Quantitative and qualitative alterations in the epidermal growth factor receptor (EGFR) commonly occur in many cancers in humans, including malignant gliomas. The aim of the current study was to evaluate molecular and cellular effects of OSI-774, a novel EGFR tyrosine kinase inhibitor, on nine glioblastoma multiforme (GBM) cell lines. METHODS: The effects of OSI-774 on expression of EGFR messenger (m)RNA and protein, proliferation, anchorage-independent growth, and apoptosis were examined using semiquantitative reverse transcription-polymerase chain reaction, immunocytochemical analysis, Coulter counting, soft agar cloning, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling/fluorescence-activated cell sorting, respectively. All p53 genes were completely and bidirectionally sequenced. Suppression of anchorage-independent growth by OSI-774 was inversely correlated to the induction of EGFR mRNA during relative serum starvation (r = -0.74) and was unrelated to p53 status. Overall, suppression of anchorage-independent growth was a considerably stronger effect of OSI-774 than inhibition of proliferation. The extent of OSI-774-induced apoptosis positively correlated with both proliferation and anchorage-independent growth of GBM cell lines (r = 0.75 and 0.79, respectively). In a single cell line derived from a secondary GBM, exposure to concentrations of greater than or equal to 1 micromol/L resulted in a substantial net cell loss during proliferation studies. CONCLUSIONS: The induction of EGFR mRNA may constitute a cellular mechanism to counteract the inhibitory effect of OSI-774 on the anchorage-independent growth of GBM cells. In contrast, no considerable correlation could be established between baseline expression levels of EGFR (both mRNA and protein) in GBM cell lines and their biological response to OSI-774. The OSI-774 induced greater (p53-independent) apoptosis in more malignant GBM phenotypes and may be a promising therapeutic agent against secondary GBM.
- Autoren
- Marc-Eric Halatsch
- Esther E Gehrke
- Vassilios I Vougioukas
- Ingolf C Bötefür
- Farhad A-Borhani
- Thomas Efferth
- Erich Gebhart
- Sebastian Domhof
- Ursula Schmidt
- Michael Buchfelder
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/15035290
- DOI
- 10.3171/jns.2004.100.3.0523
- ISSN
- 0022-3085
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- J Neurosurg
- Schlüsselwörter
- Actins
- Apoptosis
- Cell Division
- Cell Line, Tumor
- Cell Movement
- DNA Primers
- DNA, Complementary
- ErbB Receptors
- Erlotinib Hydrochloride
- Genes, p53
- Glioblastoma
- Humans
- Immunohistochemistry
- Polymerase Chain Reaction
- Quinazolines
- RNA, Messenger
- Reverse Transcriptase Polymerase Chain Reaction
- Sprache
- eng
- Country
- United States
- Paginierung
- 523 - 533
- Datum der Veröffentlichung
- 2004
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2004
- Titel
- Inverse correlation of epidermal growth factor receptor messenger RNA induction and suppression of anchorage-independent growth by OSI-774, an epidermal growth factor receptor tyrosine kinase inhibitor, in glioblastoma multiforme cell lines.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 100
Data source: PubMed
- Beziehungen:
- Property of